IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ li-2014d-4 SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation li-2014d-2 SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. human-caki_2 Human renal cell carcinoma line Caki2 caki_2 Renal cell carcinoma cell line caki-2 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation li-2014d-5 SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. in vitro In vitro ubiquitinase assay ubiquitinase assay ubiquitinase assay anti tag western anti tag western blot anti tag western Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation li-2014d-6 SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. in vitro In vitro ubiquitinase assay ubiquitinase assay ubiquitinase assay western blot western blot Immuno blot Immuno blot Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation li-2014d-1 SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. human-caki_2 Human renal cell carcinoma line Caki2 caki_2 Renal cell carcinoma cell line caki-2 ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation li-2014d-3 SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Li G., Ci W., Karmakar S., Chen K., Dhar R., Fan Z., Guo Z., Zhang J., Ke Y., Wang L., Zhuang M., Hu S., Li X., Zhou L., Li X., Calabrese M.F., Watson E.R., Prasad S.M., Rinker-Schaeffer C., Eggener S.E., Stricker T., Tian Y., Schulman B.A., Liu J., White K.P. Cancer - Interactions investigated in the context of cancer All PTEN and DUSP constructs have been entered as being of Human origin as text above Figure 4 (and Table S1) implies this fact. X Ray Crystallography experiments use Human PTEN. However the Supplemental section entitled "Cloning and Plasmid construction" refers to amino acid mapping co-ordinates that either do not exist or could be Human or Murine. (notably DUSP7-SBCm, (189)VDSSS(193) and PTEN-SBCm1, (359)ASSST(363). The authors did not reply to a request to supply the missing species of origin info. kpwhite@uchicago.edu,liuj@big.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation spop_human Speckle-type POZ protein HIB homolog 1 Roadkill homolog 1 SPOP protein protein human Homo sapiens Human MSRVPSPPPPAEMSSGPVAESWCYTQIKVVKFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV dus7_human Dual specificity protein phosphatase 7 DUSP7 PYST2 Dual specificity protein phosphatase PYST2 protein protein human Homo sapiens Human MKNQLRGPPARAHMSTSGAAAAGGTRAGSEPGAGSGSGAGTGAGAATGAGAMPCKSAEWLQEELEARGGASLLLLDCRPHELFESSHIETAINLAIPGLMLRRLRKGNLPIRSIIPNHADKERFATRCKAATVLLYDEATAEWQPEPGAPASVLGLLLQKLRDDGCQAYYLQGGFNKFQTEYSEHCETNVDSSSSPSSSPPTSVLGLGGLRISSDCSDGESDRELPSSATESDGSPVPSSQPAFPVQILPYLYLGCAKDSTNLDVLGKYGIKYILNVTPNLPNAFEHGGEFTYKQIPISDHWSQNLSQFFPEAISFIDEARSKKCGVLVHCLAGISRSVTVTVAYLMQKMNLSLNDAYDFVKRKKSNISPNFNFMGQLLDFERTLGLSSPCDNHASSEQLYFSTPTNHNLFPLNTLEST spop_human_gene SPOP HUMAN GENE gene gene human Homo sapiens Human hif1a_human Hypoxia-inducible factor 1-alpha ARNT-interacting protein Member of PAS protein 1 Basic-helix-loop-helix-PAS protein MOP1 HIF1A MOP1 BHLHE78 PASD8 PAS domain-containing protein 8 Class E basic helix-loop-helix protein 78 protein protein human Homo sapiens Human MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN vegfa_human_gene Human VEGFA gene gene gene human Homo sapiens Human uba1_human Ubiquitin-like modifier-activating enzyme 1 Ubiquitin-activating enzyme E1 Protein A1S9 UBA1 A1S9T UBE1 protein protein human Homo sapiens Human MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ub2d2_human Ubiquitin-conjugating enzyme E2 D2 UBC5B UBCH4 UBE2D2 UBC4 UBCH5B Ubiquitin-protein ligase D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-conjugating enzyme E2(17)KB 2 PUBC1 p53-regulated ubiquitin-conjugating enzyme 1 (E3-independent) E2 ubiquitin-conjugating enzyme D2 E2 ubiquitin-conjugating enzyme D2 protein protein human Homo sapiens Human MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM pten-spop-2 1535340 n/a 1535346 unspecified role unspecified role prey prey cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1535347 unspecified role unspecified role bait bait c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false sbcm1_motif required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4B spop-pten 1535340 n/a 1535347 unspecified role unspecified role prey prey n/a 1535346 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4C pten-spop-5 1535340 n/a 1535348 unspecified role unspecified role bait bait c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ser192-194gly required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1535346 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5A spop-dusp7 1535340 n/a 1535348 unspecified role unspecified role prey prey n/a 1535346 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cyto_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5B hif1a-spop-1 1535341 n/a 1535349 unspecified role unspecified role prey prey region_of_intron_1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1535350 unspecified role unspecified role bait bait association association false false false Supp. 1A Q16665 increases expression at RNA level of ENSG00000121067 hypoxia Q16665 increases expression at protein level of ENSG00000121067 hif1a-spop-2 1535341 n/a 1535350 unspecified role unspecified role bait bait n/a 1535351 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp. 1Ai hypoxic conditions Q16665 increases expression at protein level of ENSG00000112715 Q16665 increases expression at RNA level of ENSG00000112715 pten-spop-10 1535342 n/a 1535346 enzyme enzyme neutral component neutral component unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1535347 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sbcm1_motif required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 1535352 unspecified role unspecified role ancillary ancillary n/a 1535353 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1535354 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination false false false 4G, Supp 4C. P60484 ubiquitinylated lysine dusp7-spop-3 1535342 n/a 1535346 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1535348 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 1535352 unspecified role unspecified role ancillary ancillary n/a 1535353 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1535354 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination false false false 5F Q16829 ubiquitinylated lysine dusp7-spop-5 1535343 n/a 1535348 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ser192-194gly required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 1535346 enzyme enzyme neutral component neutral component unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false possible tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1535352 unspecified role unspecified role ancillary ancillary n/a 1535353 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1535354 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination false false false Supp 5C Q16829 ubiquitinylated lysine Image text states that:- "Ubiquitination assay was performed as Figure 5H." It has been assumed that Figure 5F is intended here, as there is no Figure 5H. dusp7-spop-7 1535343 n/a 1535352 unspecified role unspecified role ancillary ancillary n/a 1535353 unspecified role unspecified role ancillary ancillary n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1535354 unspecified role unspecified role ancillary ancillary ecoli Escherichia coli (strain K12) n/a 1535348 enzyme target enzyme target substrate substrate neutral component neutral component region ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 1535346 enzyme enzyme neutral component neutral component possible tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false unknown_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) ubiquitination ubiquitination reaction ubiquitination false false false Q16829 ubiquitinylated lysine 5F, Supp. 5C. hif1a-spop 1535344 n/a 1535350 unspecified role unspecified role bait bait n/a 1535349 unspecified role unspecified role prey prey region_of_intron_1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1B pten-spop 1535345 n/a 1535346 unspecified role unspecified role neutral component neutral component math_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1535347 unspecified role unspecified role neutral component neutral component sbc_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1535440 1535437 direct interaction direct interaction false false false 4A, Supp 4B 2.0A Rwork =19.2 RFree =22.1