MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014u-8 Understanding Molecular Recognition of Promiscuity of Thermophilic Methionine Adenosyltransferase, sMAT from Sulfolobus solfataricus in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined georgep@rice.edu; jsthorson@uky.edu georgep@rice.edu,jsthorson@uky.edu 2014 imex curation Wang F., Singh S., Zhang J., Huber T.D., Helmich K.E., Sunkara M., Hurley K.A., Goff R.D., Bingman C.A., Morris A.J., Thorson J.S., Phillips G.N. FEBS J. (1742-464X) Only protein-protein interactions metk_sacs2 S-adenosylmethionine synthase mat Methionine adenosyltransferase SSO0199 protein protein sulso Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) MRNINVQLNPLSDIEKLQVELVERKGLGHPDYIADAVAEEASRKLSLYYLKKYGVILHHNLDKTLVVGGQATPRFKGGDIIQPIYIIVAGRATTEVKTESGIDQIPVGTIIIESVKEWIRNNFRYLDAERHVIVDYKIGKGSSDLVGIFEASKRVPLSNDTSFGVGFAPLTKLEKLVYETERHLNSKQFKAKLPEVGEDIKVMGLRRGNEVDLTIAMATISELIEDVNHYINVKEQVRNQILDLASKIAPGYNVRVYVNTGDKIDKNILYLTVTGTSAEHGDDGMTGRGNRGVGLITPMRPMSLEATAGKNPVNHVGKLYNVLANLIANKIAQEVKDVKFSQVQVLGQIGRPIDDPLIANVDVITYDGKLTDETKNEISGIVDEMLSSFNKLTELILEGKATLF mat-1 1520964 n/a sMAT 1520965 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction false false false f3 t1 The crystal structures of sMAT have been determined successfully at 2.19 Å or 2.39 Å resolution for the AdoMet-liganded form, 2.49 Å for the AdoEth-liganded form and 2.21 Å for the unliganded form (Table 1). sMAT packs as a tetramer (Fig. 3A). All four sMAT structures contain two subunits, A and B, in the asymmetric unit and the tetramer is formed by a 2-fold crystallographic symmetry axis