MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014u-1 Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined spoegge@gwdg.de 2014 imex curation Lehneck R., Neumann P., Vullo D., Elleuche S., Supuran C.T., Ficner R., Pöggeler S. FEBS J. (1742-464X) Only protein-protein interactions c1l335_sorma Carbonic anhydrase cas1 Carbonate dehydratase protein protein sorma Sordaria macrospora MSPENTFHYALSSNNAWAGYKAHQNPHFFPKLAGGQAPEILWIGCSDSRCPETTILGMQPGDVFVHRNIANIVSPTDINTTAVIEYAVAHLKVKHIVLCGHSACGGAAGALSDGRIGGVLDTWLLPLKTVRYNHAEELDAITDEKERVIRIAQLNVEAGIKVLMNNPTIREAIAERGLEVHGVFFDIGCGRIKELGCGTAHKSSSTISGDHVVRGKHGQLVFGQDGEAEIAAAQ cas1 1480585 n/a CAS1 1480586 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false t3 CAS1 crystallized in space group P212121 with two monomers occupying the asymmetric unit. The structure was refined at 2.7 A, to crystallographic R factors of 20.4% and 24.25.1% for Rwork and Rfree, respectively. Analysis of crystal contacts using the PISA software [36] suggested that the biologically active molecule is a homo-tetramer possessing D2 symmetry (Fig. 2A). CAS2 crystallized in the F222 space group with one monomer occupying the asymmetric unit, and the structure was refined at 1.8 A, to final Rwork of 19. 1% and Rfree of 21. 1%. Crystal contact analysis indicated an equivalent homo-tetrameric oligomerization state to CAS1