MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014o-1 Structural and kinetic studies on Adenylosuccinate Lyase from Mycobacterium smegmatis and Mycobacterium tuberculosis provide new insights on the catalytic residues of the enzyme in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined mrn@mbu.iisc.ernet.in 2014 imex curation Banerjee S., Agrawal M.J., Mishra D., Sharan S., Balaram H., Savithri H.S., Murthy M.R. FEBS J. (1742-464X) Only protein-protein interactions a0r4i6_mycs2 Adenylosuccinate lyase purB Adenylosuccinase MSMEG_5847 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MFVTIPNVLANRYASDEMVAIWSPEAKIIAERRLWLAVLRAQAELGVAVPDGVVEDYERVLENVDLESIAARERVTRHDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRQSLELVFSHGVAVVARLAERAVVYRDLVMAGRSHNVAAQATTLGKRFASAAEETLVALTRLRELIDRYPLRGVKGPMGTAQDMLDLFGGDVGKLADLERRVAEFLGFTEVFTSVGQVYPRSLDHDVLSALVQFGAGPSSMAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYASMAAELAGAQWNEGDVFCSVVRRVALPDAFFAIDGQTETFLTVLDEFGAYPAVIQRELDRYLPFLATTRILMAAVRAGVGREAAHEVIKEHAVAVALAMREQGREPDLIDRLAGDPRLPLDKVALEAALEDKQAFTGAAGDQVDGVVAAVGELVSRYPEAAKYTSGAIL purb-1 1479194 n/a 1479195 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction false false false t1 f3 X-ray crystal structure of Mycobacterium smegmatis apo-adenylosuccinate lyase (ASL) with a partially ordered active site loop was determined at 2.16 Å resolution. The structure suggests that His149, Lys285 and Ser279 are functionally important. The low catalytic activity observed for M. smegmatis and M. tuberculosis ASLs is consistent with their slow growth rate