MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint pruneda-2014-4 E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pruneda J.N., Smith F.D., Daurie A., Swaney D.L., Villén J., Scott J.D., Stadnyk A.W., Le Trong I., Stenkamp R.E., Klevit R.E., Rohde J.R., Brzovic P.S. brzovic@uw.edu EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation pruneda-2014-5 E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Pruneda J.N., Smith F.D., Daurie A., Swaney D.L., Villén J., Scott J.D., Stadnyk A.W., Le Trong I., Stenkamp R.E., Klevit R.E., Rohde J.R., Brzovic P.S. brzovic@uw.edu EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation pruneda-2014-1 E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pruneda J.N., Smith F.D., Daurie A., Swaney D.L., Villén J., Scott J.D., Stadnyk A.W., Le Trong I., Stenkamp R.E., Klevit R.E., Rohde J.R., Brzovic P.S. brzovic@uw.edu EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation pruneda-2014-2 E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Pruneda J.N., Smith F.D., Daurie A., Swaney D.L., Villén J., Scott J.D., Stadnyk A.W., Le Trong I., Stenkamp R.E., Klevit R.E., Rohde J.R., Brzovic P.S. brzovic@uw.edu EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation pruneda-2014-3 E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. human-caco2 Human Caucasian colon adenocarcinoma caco2 Human Caucasian colon adenocarcinoma pull down pull down ms participant Identification by mass spectrometry ms participant Pruneda J.N., Smith F.D., Daurie A., Swaney D.L., Villén J., Scott J.D., Stadnyk A.W., Le Trong I., Stenkamp R.E., Klevit R.E., Rohde J.R., Brzovic P.S. brzovic@uw.edu EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation pruneda-2014-6 E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Pruneda J.N., Smith F.D., Daurie A., Swaney D.L., Villén J., Scott J.D., Stadnyk A.W., Le Trong I., Stenkamp R.E., Klevit R.E., Rohde J.R., Brzovic P.S. brzovic@uw.edu EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation ub2d1_human Ubiquitin-conjugating enzyme E2 D1 Ubiquitin-protein ligase D1 Ubiquitin carrier protein D1 UbcH5 Ubiquitin-conjugating enzyme E2-17 kDa 1 Ubiquitin-conjugating enzyme E2(17)KB 1 UBC4/5 homolog Stimulator of Fe transport UBE2D1 UBCH5 UBCH5A SFT UBC5A (E3-independent) E2 ubiquitin-conjugating enzyme D1 E2 ubiquitin-conjugating enzyme D1 protein protein human Homo sapiens Human MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM ospg_shifl Protein kinase OspG ospG Effector protein OspG pWR501_0237 SFLP087 CP0227 protein protein shifl Shigella flexneri MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFEDVNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVPGTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRNIYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL ub2d2_human Ubiquitin-conjugating enzyme E2 D2 UBC5B UBCH4 UBE2D2 UBC4 UBCH5B Ubiquitin-protein ligase D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-conjugating enzyme E2(17)KB 2 PUBC1 p53-regulated ubiquitin-conjugating enzyme 1 (E3-independent) E2 ubiquitin-conjugating enzyme D2 E2 ubiquitin-conjugating enzyme D2 protein protein human Homo sapiens Human MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM ub2d3_human Ubiquitin-conjugating enzyme E2 D3 UBC5C Ubiquitin-protein ligase D3 Ubiquitin carrier protein D3 Ubiquitin-conjugating enzyme E2-17 kDa 3 Ubiquitin-conjugating enzyme E2(17)KB 3 UBE2D3 UBCH5C (E3-independent) E2 ubiquitin-conjugating enzyme D3 E2 ubiquitin-conjugating enzyme D3 protein protein human Homo sapiens Human MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM ub2e1_human Ubiquitin-conjugating enzyme E2 E1 UBE2E1 UBCH6 Ubiquitin-protein ligase E1 Ubiquitin carrier protein E1 UbcH6 (E3-independent) E2 ubiquitin-conjugating enzyme E1 E2 ubiquitin-conjugating enzyme E1 protein protein human Homo sapiens Human MSDDDSRASTSSSSSSSSNQQTEKETNTPKKKESKVSMSKNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT ub2e2_human Ubiquitin-conjugating enzyme E2 E2 UBE2E2 UBCH8 Ubiquitin-protein ligase E2 Ubiquitin carrier protein E2 UbcH8 E2 ubiquitin-conjugating enzyme E2 protein protein human Homo sapiens Human MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT ub2e3_human Ubiquitin-conjugating enzyme E2 E3 UBE2E3 UBCE4 Ubiquitin-protein ligase E3 Ubiquitin carrier protein E3 Ubiquitin-conjugating enzyme E2-23 kDa UbcH9 UBCH9 E2 ubiquitin-conjugating enzyme E3 protein protein human Homo sapiens Human MSSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT ube2k_human Ubiquitin-conjugating enzyme E2 K HIP2 Ubiquitin-conjugating enzyme E2-25 kDa Ubiquitin-protein ligase Ubiquitin carrier protein Huntingtin-interacting protein 2 UBE2K LIG E2 ubiquitin-conjugating enzyme K protein protein human Homo sapiens Human MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN ube2n_human Ubiquitin-conjugating enzyme E2 N Ubiquitin-protein ligase N Ubiquitin carrier protein N Ubc13 Bendless-like ubiquitin-conjugating enzyme UBE2N BLU UbcH13 E2 ubiquitin-conjugating enzyme N protein protein human Homo sapiens Human MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI ub2l3_human Ubiquitin-conjugating enzyme E2 L3 Ubiquitin-protein ligase L3 Ubiquitin carrier protein L3 UbcH7 Ubiquitin-conjugating enzyme E2-F1 L-UBC UBE2L3 UBCE7 UBCH7 E2 ubiquitin-conjugating enzyme L3 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD ub2l6_human Ubiquitin/ISG15-conjugating enzyme E2 L6 UBE2L6 UBCH8 Ubiquitin-protein ligase L6 Ubiquitin carrier protein L6 UbcH8 Retinoic acid-induced gene B protein E2 ubiquitin-conjugating enzyme L6 protein protein human Homo sapiens Human MMASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS ubc_human Polyubiquitin-C UBC protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGV ospg-ube2d1-1 1531076 n/a 1531082 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false t2 sf2b ospg-ube2d1-2 1531076 n/a 1531084 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false t2 sf2b ospg-ube2d2-2 1531076 n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1531085 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified P61077:p.Phe62Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false t2 sf2b f3b sf5b ospg-ube2d3 1531076 n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1531086 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified physical association physical association false false false t2 sf2b ospg-ube2e1 1531076 n/a 1531087 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false t2 sf2b ospg-ube2e2 1531076 n/a 1531088 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false t2 sf2b ospg-ube2e3 1531076 n/a 1531089 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false t2 sf2b ospg-ube2k 1531076 n/a 1531090 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey purified purified n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false t2 sf2b ospg-ube2n 1531076 n/a 1531091 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false t2 sf2b ospg-ube2l3 1531076 n/a 1531083 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1531092 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role prey prey partially purified partially purified physical association physical association false false false t2 sf2b ospg-ubc-1 1531077 n/a 1531093 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1531083 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1531085 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false f4b sf4b ospg-ubc-2 1531077 n/a 1531085 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1531093 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction false false false f4a ospg-ubc 1531078 n/a 1531083 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1531085 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1531093 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false f1 t1 sf1 we determined the crystal structure of the OspG/UbcH5c-O~Ub complex to 2.70 A (Fig 1A and Table 1). The covalent oxyester linkage between the UbcH5c active site (mutated from cysteine to serine) and the UbC-terminus is maintained in the complex (Supplementary Fig S1A) and binding to OspG does not significantly alter the conformations of either UbcH5c or Ub. ospg-ube2d2 1531079 n/a 1531092 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531091 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531086 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531085 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531084 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531083 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false t2 ospg-ube2d2-1 1531080 n/a 1531085 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531086 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531091 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531083 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1531087 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531084 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1531089 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association false false false t2 ospg-ubc-3 1531081 n/a 1531085 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1531083 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1531093 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false f5a