IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mahul_mellier-2014-3 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. in vitro In vitro protein kinase assay protein kinase assay tag visualisation tag visualisation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Method was found in Pluk etal.,2002, pmid:11832214. Purified Abl protein was bound to protein G-Sepharose beads. y-32P-ATP, ATP were added in buffer, and after incubation, SDS-PAGE bands from the gel underwent scintillation counting and/or phosphoimager analysis. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-8 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-1 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. in vitro In vitro protein kinase assay protein kinase assay fingerprinting peptide massfingerprinting fingerprinting Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-2 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-4 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. alpha-Syn was purified from E.coli grown in isotopically supplemented medium. "Real-Time NMR":- Sequential 1 h 1H–15N HSQC spectra were collected overnight and the relative intensity of the peaks arising from unphosphorylated and phosphorylated protein was calculated and plotted for well-resolved resonances corresponding to residues near to Y39 (Leu38 and Gly41). -the appearance of new cross-peaks for residues L38 and G41 (among others) reports on the phosphorylation at Y39. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-5 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. mouse-cerebral_cortical_neuron Mouse cerebral cortical neuron brain-cortex_neuron Cerebral cortical neuron confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-6 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-7 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. mouse-cerebral_cortical_neuron Mouse cerebral cortical neuron brain-cortex_neuron Cerebral cortical neuron anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-9 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease mahul_mellier-2014-10 c-Abl phosphorylates α-syn and regulates its degradation, implication for α-syn clearance and contribution to the pathogenesis of Parkinson's Disease. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation Parkinsons - Interactions investigated in the context of Parkinsons disease p00519-1 Tyrosine-protein kinase ABL1 ABL1 ABL JTK7 Abelson murine leukemia viral oncogene homolog 1 Abelson tyrosine-protein kinase 1 Proto-oncogene c-Abl p150 protein protein human Homo sapiens Human MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA crkl_human Crk-like protein CRKL protein protein human Homo sapiens Human MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE p46108-1 Adapter molecule crk Proto-oncogene c-Crk CRK p38 protein protein human Homo sapiens Human MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFS p00519-2 Tyrosine-protein kinase ABL1 ABL1 ABL JTK7 Abelson murine leukemia viral oncogene homolog 1 Abelson tyrosine-protein kinase 1 Proto-oncogene c-Abl p150 protein protein human Homo sapiens Human MGQQPGKVLGDQRRPSLPALHFIKGAGKKESSRHGGPHCNVFVEHEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR Contains a N-myristoyl glycine at position 2. syua_mouse Alpha-synuclein Snca Syn Non-A beta component of AD amyloid Non-A4 component of amyloid precursor protein protein mouse Mus musculus Mouse MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA abl1_mouse Tyrosine-protein kinase ABL1 Abl1 Abl Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 Abelson tyrosine-protein kinase 1 protein protein mouse Mus musculus Mouse MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDTESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDIVRR abl1-snca-2 1479653 n/a 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1479664 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false tyr-39 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation false false false 1479653 1C Y39 peptide Vmax = 13 pmol/min/ug abl1-snca-3 1479653 n/a 1479665 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1479653 1C-2 P46109 tyr-207 O-phospho-L-tyrosine Y207 peptide Vmax = 36 pmol/min/ug positive control interaction abl1-snca-4 1479653 n/a 1479664 enzyme target enzyme target substrate substrate unspecified role unspecified role P37840:p.Tyr39Phe mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1D, suppl fig. 2A P37840 ?-? O-phospho-L-tyrosine abl1-snca-11 1479653 n/a 1479666 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) n/a 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Supp 2A p46108-1 O-phospho-L-tyrosine abl1-snca-14 1479653 N/A 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1479664 enzyme target enzyme target substrate substrate unspecified role unspecified role n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tyr-125 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation false false false 1479653 1Ci Y125 peptide Vmax = 21pmol/min/ug abl1-snca-8 1479654 n/a 1479664 unspecified role unspecified role bait bait possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false n/a 1479667 unspecified role unspecified role prey prey physical association physical association false false false When c-Abl KD ( lys290arg mutation) was transfected rather than WT c-Abl, a band is clearly visible when an anti-c-Abl antibody is used for detection;however the c-Abl detected is not phosphorylated at Y39 or Y125. However the text stated that "co-immunoprecipitations of alpha-syn with the c-Abl kinase-dead mutant (KD c-Abl) revealed a lack of interaction between the two proteins". 8B/F/G a-synuclein was phosphorylated at Y39 only subsequent to c-Abl transfection. Y125 was already phosphorylated in control non-c-Abl transfected samples, wheras Y39 was not. Mutating Y39, Y125 or both residues to phenylalanine in a-syn did not affect its interaction with c-Abl but only its phosphorylation status. abl1-snca-10 1479654 n/a 1479664 unspecified role unspecified role prey prey possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false n/a 1479667 unspecified role unspecified role bait bait physical association physical association false false false 8E abl1-snca-13 1479655 n/a 1479664 enzyme target enzyme target substrate substrate unspecified role unspecified role P37840:p.[Tyr39Phe;Tyr125Phe] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P37840:p.Tyr39Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1A P37840 O-phospho-L-tyrosine abl1-snca-1 1479656 n/a 1479664 enzyme target enzyme target substrate substrate unspecified role unspecified role P37840:p.[Tyr39Phe;Tyr125Phe] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P37840:p.Tyr39Phe mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false undefined mutation mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1B P37840 tyr-39 O-phospho-L-tyrosine P37840 tyr-125 O-phospho-L-tyrosine abl1-snca-5 1479657 n/a 1479663 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1479664 enzyme target enzyme target substrate substrate unspecified role unspecified role region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 1E/F, Supp 2B. P37840 tyr-39 O-phospho-L-tyrosine abl1-snca-6 1479658 n/a 1479668 unspecified role unspecified role neutral component neutral component possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false n/a 1479667 unspecified role unspecified role neutral component neutral component P00519-2:p.Lys290Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 5B, Supp3B. abl1-snca-7 1479659 n/a 1479664 unspecified role unspecified role neutral component neutral component possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false n/a 1479667 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6C snca-abl1-1 1479660 n/a 1479669 unspecified role unspecified role prey prey n/a 1479668 unspecified role unspecified role bait bait physical association physical association false false false 8A abl1-snca-9 1479661 n/a 1479667 unspecified role unspecified role prey prey n/a 1479664 unspecified role unspecified role bait bait possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 8C/D abl1-snca-12 1479662 n/a 1479667 unspecified role unspecified role neutral component neutral component n/a 1479664 unspecified role unspecified role neutral component neutral component possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Supp 4C