UniProt UniProt http://www.uniprot.org bagchi-2013-1 Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Virology (0042-6822) 2013 imex curation Only protein-protein interactions imex curation bagchi-2013-2 Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Virology (0042-6822) 2013 imex curation Only protein-protein interactions imex curation bagchi-2013-3 Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Virology (0042-6822) 2013 imex curation Only protein-protein interactions imex curation traf2_human TNF receptor-associated factor 2 TRAF2 TRAP3 Tumor necrosis factor type 2 receptor-associated protein 3 E3 ubiquitin-protein ligase TRAF2 RING-type E3 ubiquitin transferase TRAF2 protein protein human Homo sapiens Human MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTYDGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL p89055_9reov Non-structural protein 1 NSP1 NCVP2 Non-structural RNA-binding protein 53 protein protein rotavirus sp. Rotavirus sp. MATFKDACYHYRKLNKLNGLVLKLGANDAWRPAPIAKYKGWCLDCCQHTDLTYCRGCALFHVCQWCSQYNRCFLDEEPHLLRMRTFRNQISRKDIEGLINMYNTLFPINERIVDKFISNVKQRRCRNEFLIEWYNHLLLPITLQALMVELEGDVYYIFGYFDHMEKENQTPFQFVNMINNYDKLLLDDKNFDRMTNLPVILQQEYAFRYFSKSRFISKTKKSVNRHDFANNLMEEMDNPISLMQVMRNCVNEYMDDKNWNEKCTLIVDMKSYMELMKSSYTEHYSVSQRCKLFTIYKLNIISKLIKPNYIFSNHGAHALDVHNCKWCQMNNHYKIWDDFRLKKVYNNTMCFIRALMKSNKNVGHRSSQEVVYEYMSNIFIVWKNEKWNKSMQMIFDYLEPVEISGIEYILLDHELSWEIRGIVMQIMNGEIPRILTFNDVKKIISAIIYDWFDVRYMREMPLIISTTNELRKMNKRNDLMDEYSYELSDTE traf2-nsp1-1 1539024 n/a 1539027 unspecified role unspecified role bait bait n/a 1539028 unspecified role unspecified role prey prey infection infection physical association physical association false false false 2A nsp1-traf2-1 1539025 n/a 1539027 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1539028 unspecified role unspecified role bait bait c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2B and D nsp1-traf2-2 1539026 n/a 1539027 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1539028 unspecified role unspecified role neutral component neutral component c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 2C