UniProt UniProt http://www.uniprot.org nehru-2013-1 Interaction of RhoD and ZIP kinase modulates actin filament assembly and focal adhesion dynamics. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nehru V., Almeida F.N., Aspenström P. Origin of RhoD fo Y2H screen according ref. 14 (pmid 23087206). pontus.aspentrom@ki.se human mammary gland cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2013 imex curation Only protein-protein interactions nehru-2013-2 Interaction of RhoD and ZIP kinase modulates actin filament assembly and focal adhesion dynamics. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nehru V., Almeida F.N., Aspenström P. Origin of RhoD fo Y2H screen according ref. 14 (pmid 23087206). pontus.aspentrom@ki.se human mammary gland cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2013 imex curation Only protein-protein interactions dapk3_human Death-associated protein kinase 3 DAPK3 ZIPK DAP-like kinase Zipper-interacting protein kinase MYPT1 kinase protein protein human Homo sapiens Human MSTFRQEDVEDHYEMGEELGSGQFAIVRKCRQKGTGKEYAAKFIKKRRLSSSRRGVSREEIEREVNILREIRHPNIITLHDIFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHKIEAGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWIKAIRRRNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSYADFERFSKVLEEAAAAEEGLRELQRSRRLCHEDVEALAAIYEEKEAWYREESDSLGQDLRRLRQELLKTEALKRQAQEEAKGALLGTSGLKRRFSRLENRYEALAKQVASEMRFVQDLVRALEQEKLQGVECGLR rhod_human Rho-related GTP-binding protein RhoD RHOD ARHD Rho-related protein HP1 protein protein human Homo sapiens Human MTAAQAAGEEAPPGVRSVKVVLVGDGGCGKTSLLMVFADGAFPESYTPTVFERYMVNLQVKGKPVHLHIWDTAGQDDYDRLRPLFYPDASVLLLCFDVTSPNSFDNIFNRWYPEVNHFCKKVPIIVVGCKTDLCKDKSLVNKLRRNGLEPVTYHRGQEMARSVGAVAYLECSARLHDNVHAVFQEAAEVALSSRGRNFWRRITQGFCVVT rhod_mouse Rho-related GTP-binding protein RhoD Rhod Arhd Rhom protein protein mouse Mus musculus Mouse MNASQVAGEEAPQSGHSVKVVLVGDGGCGKTSLMMVFAKGAFPESYSPTVFERYNATLQMKGKPVHLQIWDTAGQDDYDRLRPLFYPDANVLLLCFDVTNPNSFDNVSNRWYPEVTHFCKGVPIIVVGCKIDLRKDKVLVNNLRKKRLEPVTYHRGHDMARSVGAVAYLECSARLHDNVEAVFQEAAEVALSSRRHNFWRRITQNCCLAT mint Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Participates in reorganization of actin cytoskeleton rhod-dapk3 1565385 n/a 1565387 unspecified role unspecified role prey prey n/a 1565388 unspecified role unspecified role bait bait O00212:p.Gly26Val mutation mutation certain certain sequence position certain certain certain sequence position certain false renders RhoD constitutively active physical association physical association false false false no figure rhod-dapk3-3 1565386 n/a 1565389 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection P97348:p.Thr31Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false RhoD dominant negative mutant region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1565387 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O43293:p.Asp161Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B and C RhoD wildtype and in GTP-bound form interacted (transfected RhoD G26V). DAPK3/ZIPK mutant construct lacking the leucine zipper domain interacted with RhoD wildtype and RhoD GDP- and GTP-bound forms ( transfected RhoD T31N and RhoD G26V, respectively).