MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint chiba-2013-1 NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec15. human-rpe Human retinal pigment epithelium cell line rpe retinal pigmented epithelial cells fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Chiba S., Amagai Y., Homma Y., Fukuda M., Mizuno K. kmizuno@biology.tohoku.ac.jp EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation chiba-2013-3 NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec15. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Chiba S., Amagai Y., Homma Y., Fukuda M., Mizuno K. kmizuno@biology.tohoku.ac.jp EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation chiba-2013-2 NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec15. human-rpe Human retinal pigment epithelium cell line rpe retinal pigmented epithelial cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chiba S., Amagai Y., Homma Y., Fukuda M., Mizuno K. kmizuno@biology.tohoku.ac.jp EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation chiba-2013-4 NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec15. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chiba S., Amagai Y., Homma Y., Fukuda M., Mizuno K. kmizuno@biology.tohoku.ac.jp EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation rab3i_rat Rab-3A-interacting protein Rab3ip Rabin3 Rabin-3 SSX2-interacting protein Rabin8 protein protein rat Rattus norvegicus (Rat) Rat MANDPLEGFHEVNLASPTSPDLLGVCDPGTQEQTTSPSVIYRPHPSTLCSATIQANALNLSDLPTQPVYSSPRHLNCAEISNISIHVPEPASSVASEVAAGLTRFTSRKDSCNAEREFLQGATVTEASAGNDDIFGLSTDSLSRLRSPSVLEVREKGYERLKEELAKAQRELKLKDEECERLSKVRDQLGQELEELTASLFEEAHKMVREANVKQATAEKQLKEAQGKIDVLQAEVAALKTLVLSSSPTSPTQEPLAAGKTPFKRGHTRNKSTSSAMSGSHQDFSAIQPIVKDCREADLSLYNEFRSWKDEPTMDRTCPFLDKIYQEDIFPCLTFAKSELASAVLEAVENNTLSIEPVGLQPIRFVKASAVECGGPKKCALTGQSKPCKHRIKLGDSSSYYYISPVCRYRITSVCNFFTYIRYIQQGLVKQQDVDQMFWEVMQLRKEMSLAKLGYFKEEL rb11a_mouse Ras-related protein Rab-11A Rab11a Rab11 protein protein mouse Mus musculus Mouse MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI exoc6_mouse Exocyst complex component 6 Exoc6 Sec15a Sec15l1 Exocyst complex component Sec15A SEC15-like protein 1 protein protein mouse Mus musculus Mouse MAESCEALGTVPEHERILQEIESTDTACVGPTLRSVYDDQPNAHKKFMEKLDACIRNHDKEIEKMCNFHHQGFVDAITELLKVRADAEKLKVTDTNRRFQDAGKEVIIQTEDIIRCRIQQRNITTVVEKLQLCLPVLEMYSKLKEQMSMKRYYSALKTMEQLENVYFPRVSQYRFCQLMMETLPKLREDMMNYCMSDLTYGLESIRKHSDKIGEAAMKQAQQQKSFSVALQKQNNMRFGKNMHVNNDRILEEKSDVIPKHALEEEAENDEEVLTVQDLVDFSPVYRCLHIYSALGDEETFENYYRKQRKKQARLVLQPQSSVHETVDGYRRYFTQIVGFFVVEDHILHVTQGLVTRVYTEELWNMALSKIIAVLRAHSSYCTDPDLVLELKNLIVIFADTLQGYGFPVNRLFDLLFEIRDQYNETLLKKWAGIFRDIFEEDNYSPIPIGSEEEYKVVISRFPFQDPDLEKQSFPKKFPMSQSVPLIYIQVKEFIYASLKFSESLHRSSTEIDDMLRKSTNLLLTRILSSCLLNLIRKPHIGLTELVQIIINTTHLEQACKYLEDFITNITNISQETVHTTRLYGLSTFKDARHAAEGEIYTKLNQKIDEFVQLADYDWTMAESDGRASGYLMDLINFLRSIFQVFTHLPGKVAQTACMSACQHLSTSLMQMLLDSELKQISMGAVQQFNLDVIQCELFASSEPVPGFQGDTLQLAFIDLRQLLDLFMVWDWSTYLADYGQPASKYLRVNPHAALTLLEKMKDTSKKNNIFAQFRKNDRDRQKLIETVVRQLRGLVTGMSQHT rab11a-rab3ip-1 1497671 n/a 1497675 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q62739:p.Ser272Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false n/a 1497676 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f4b When YFP-Rabin8(WT) or the S272A mutant was coexpressed with CFP-Rab11(QL), Rabin8(S272A) was more abundantly accumulated on the Rab11(QL)-containing vesicles around the centrosome than Rabin8(WT) (Figure 4B). rab11a-rab3ip-2 1497671 n/a 1497676 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1497675 anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q62739:p.Ile329Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false f6g sf4b Rabin8(I329S) was distributed diffusely in the cytoplasm and did not colocalize with Rab11(QL) on pericentrosomal vesicles in RPE1 cells (Figure 6G), indicating that binding to Rab11 and/or PS is required for Rabin8 recruitment and trafficking to the pericentrosome. Rabin8 colocalized with Rab11(WT) or Rab11(QL) on pericentrosomal and endosomal vesicles but diffusely distributed in the cytoplasm in cells expressing Rab11(SN) (Supplementary Figure S4B). exoc6-rab3ip-1 1497672 n/a 1497677 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1497675 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q62739:p.Ile329Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false f6d exoc6-rab3ip-2 1497672 n/a 1497675 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait purified purified Q62739:p.Ile329Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1497676 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f6d sf4a sf4c Rabin8(I329S) lost the ability to bind to Rab11(QL) and exhibited increased binding to Sec15 (Figure 6D). Rabin8 bound to Rab11(WT) and GTP hydrolysis-deficient Rab11(QL), but not to GDPlocked Rab11(SN) (Supplementary Figure S4A). GST pull-down assays showed that Rab11(QL) bound to Rabin8(WT) and to the S272A and S272E mutants to a similar extent (Supplementary Figure S4C), indicating that Rabin8 phosphorylation has no apparent effect on its binding to Rab11- GTP, at least in in vitro binding assays. exoc6-rab3ip 1497673 n/a 1497677 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1497675 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q62739:p.Ser272Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false f6b rab3ip-rab11a-2 1497674 n/a 1497675 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait partially purified partially purified tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1497676 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false sf4d When the post-nuclear supernatants of cells expressing Rab11(QL) or Rab11(QL, 2CA) were subjected to coimmunoprecipitation assays with Rabin8, Rabin8 coprecipitated Rab11(QL) and Rab11(QL, 2CA) to a similar extent (Supplementary Figure S4D), indicating that the C-terminal lipid modification of Rab11 does not affect its binding to Rabin8.