IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hain-2012-3 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. In some SPR experiments, a triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation hain-2012-4 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. A triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation hain-2012-1 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. A triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation hain-2012-2 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. A triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation hain-2012-5 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. His6-PfAtg8 was attached to Ni-NTA resin and incubated with red blood cell stage P. falciparum lysate. In some pull down experiments, a triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation hain-2012-6 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation hain-2012-7 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation hain-2012-8 Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation c0h519_plaf7 PF3D7_0905700.2 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MSDQINVKHKIGDTCRKLYSYFKTVNNTSTFIQNGTLTPSEFVDSGDFLVYKFKTWEWQEADKDRVVPYLPENKQFLITKNVPCKQRIKDLNNIVHDLKIVDNDWLLPSYEEDNNPTDIYEYLPNSEYTINDKNIYNYEEEEEDDNCDEAIDINNFYMENNLIKEHDPASINSTSCYSKNMLHDNLMKIRTYDVSITYDKYYQTPRIWLFGYNENGDPLKSEEIFEDILSDYSYKTVTYDPHPCTGVMTASIHPCKHAEAILNVVNNWISEEKEPRHDLYLLFLLKFISGVIPTIEYDFTTDIEIPRDSNAGL q8ijk2_plaf7 Autophagy-related protein PF3D7_1019900 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MPSLKDEVSFENRVAETHKIRSKYPNRIPVVCERANRSNLPIIEKKKFLVPMNMLVGEFKFILHQHINQSAYGSNMKLFRERTIYLFVNNIVPKTGLLMQDLYEMYKDEDGYLYMEYSCESCLG benzene-1,2,4-triol benzene-1,2,4-triol 1,2,4-benzenetriol 1,2,4-Benzenetriol 1,2,4-Trihydroxybenzene Benzene-1,2,4-triol Hydroxyhydroquinone Hydroxyquinol benzene-1,2,4-triol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) GGNQRNBDZQJCCN-UHFFFAOYSA-N pyrogallol pyrogallol 1,2,3-Benzenetriol 1,2,3-Trihydroxybenzene benzene-1,2,3-triol Pyrogallic acid Pyrogallol benzene-1,2,3-triol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) WQGWDDDVZFFDIG-UHFFFAOYSA-N 4-nitrocatechol 4-nitrocatechol 1,2-Dihydroxy-4-nitrobenzene 4-Nitrocatechol 4-nitrocatechol OK 4-Nitropyrocatechol 4-nitrobenzene-1,2-diol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) XJNPNXSISMKQEX-UHFFFAOYSA-N 1,3-indandione 1,3-indandione small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) esculetin esculetin 6,7-dihydroxy-2H-1-benzopyran-2-one 6,7-dihydroxycoumarin aesculetin cichorigenin cichoriin aglucon cichoriin aglycon esculetin Esculetin esculin aglucon esculin aglycon 6,7-dihydroxy-2H-chromen-2-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ILEDWLMCKZNDJK-UHFFFAOYSA-N nbd-chloride 4-Chloro-7-nitrobenzofurazan small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) c0h519-q8ijk2-2 1540385 n/a PfAtg3 1540393 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a PfAtg8 1540394 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction false false false 1540385 1540385 1540385 Fig. 1d, Fig 5c, Fig. 5d, Fig. 5e Temp = 25C PfAtg3 (C0H519) binds to wild-type PfAtg8 (Q8IJK2) with Kd = 290 x 10e-9 molar, k-on = 54.3 x 10e+5 per molar per second, and k-off = 0.13 per second. PfAtg3 (C0H519) binds to the triple cys mutant of PfAtg8 (Q8IJK2) (cys32ile-cys119ser-cys122ser) with Kd = 326 x 10e-9 molar, k-on = 5.1 x 10e+5 per molar per second, and k-off = 0.17 per second. c0h519-c0h519 1540385 n/a PfAtg3 1540393 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a PfAtg3 1540393 competitor competitor competitor prey prey lig_lir_apic_2 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a PfAtg8 1540394 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 physical association physical association false false false Fig. 1d c0h519-benzene_1_2_4_triol 1540385 n/a PfAtg3 1540393 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a F408 1540395 inhibitor inhibitor prey prey n/a PfAtg8 1540394 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 physical association physical association false false false 1540385 Fig. 7b c0h519-pyrogallol 1540385 n/a PfAtg8 1540394 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a PfAtg3 1540393 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a F410 1540396 inhibitor inhibitor prey prey physical association physical association false false false 1540385 Fig. 7b q8ijk2-benzene_1_2_4_triol 1540385 n/a F408 1540395 unspecified role unspecified role prey prey n/a PfAtg8 1540394 unspecified role unspecified role bait bait Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction false false false Suppl. Fig. S9, Table 2 q8ijk2-pyrogallol 1540385 n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a F410 1540396 unspecified role unspecified role prey prey direct interaction direct interaction false false false Suppl. Fig. S9, Table 2 q8ijk2-4_nitrocatechol 1540385 n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a F537 1540397 unspecified role unspecified role prey prey direct interaction direct interaction false false false Suppl. Fig. S9, Table 2 q8ijk2-1_3_indandione 1540385 n/a F792 1540398 unspecified role unspecified role prey prey n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction false false false Suppl. Fig. S9, Table 2 q8ijk2-esculetin 1540385 n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a F268 1540399 unspecified role unspecified role prey prey direct interaction direct interaction false false false Suppl. Fig. S9, Table 2 q8ijk2-nbd_chloride 1540385 n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a F470 1540400 unspecified role unspecified role prey prey direct interaction direct interaction false false false Suppl. Fig. S9, Table 2 c0h519-pyrogallol-1 1540385 n/a F410 1540396 unspecified role unspecified role prey prey n/a PfAtg3 1540393 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction false false false Suppl. Fig. S11 q8ijk2-c0h519 1540386 n/a 1540394 unspecified role unspecified role bait bait Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a PfAtg3 1540393 unspecified role unspecified role prey prey lig_lir_apic_2 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Fig. 1c q8ijk2-esculetin-1 1540386 n/a F268 1540399 unspecified role unspecified role prey prey n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction false false false Fig. 7a q8ijk2-4_nitrocatechol-1 1540386 n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a F537 1540397 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig. 7a q8ijk2-benzene_1_2_4_triol-1 1540386 n/a F408 1540395 unspecified role unspecified role prey prey n/a 1540394 unspecified role unspecified role bait bait Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction false false false Fig. 7a c0h519-q8ijk2 1540387 n/a PfAtg3 1540393 unspecified role unspecified role neutral component neutral component lig_lir_apic_2 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 3.0 n/a PfAtg8 1540394 unspecified role unspecified role neutral component neutral component ubiquitin-related domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false gamg-tag n-terminus tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 Stoichiometry: 3.0 1540488 1540486 direct interaction direct interaction false false false Table 1 2.2A working 20.5%, free 27.9% c0h519-q8ijk2-1 1540388 n/a PfAtg8 1540394 unspecified role unspecified role prey prey Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a PfAtg3 1540393 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false C0H519:p.Trp105Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1b q8ijk2-c0h519-1 1540389 n/a PfAtg3 1540393 unspecified role unspecified role prey prey plaf7 Plasmodium falciparum (isolate 3D7) n/a PfAtg8 1540394 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 physical association physical association false false false Fig. 4b, Fig. S7 c0h519-q8ijk2-3 1540390 n/a PfAtg8 1540394 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 Stoichiometry: 1.0 n/a PfAtg3 1540393 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 Stoichiometry: 1.0 direct interaction direct interaction false false false Fig. 5b c0h519-q8ijk2-4 1540391 n/a PfAtg8 1540394 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 Stoichiometry: 1.0 n/a PfAtg3 1540393 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 Stoichiometry: 1.0 direct interaction direct interaction false false false Fig. 5a c0h519-q8ijk2-5 1540392 n/a PfAtg3 1540393 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a PfAtg8 1540394 unspecified role unspecified role prey prey Q8IJK2:p.Leu49Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8IJK2:p.Arg27Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8IJK2:p.Glu44_Lys46delinsAlaSerAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8IJK2:p.Lys47Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction false false false Fig. 6c