UniProt UniProt http://www.uniprot.org louie-2006-1 Structural determinants and modulation of substrate specificity in phenylalanine-tyrosine ammonia-lyases. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Louie G.V., Bowman M.E., Moffitt M.C., Baiga T.J., Moore B.S., Noel J.P. noel@salk.edu Chem. Biol. (1074-5521) 2006 imex curation Only protein-protein interactions taly_cers4 Tyrosine ammonia-lyase hutH RSP_3574 protein protein rhos4 Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) MLAMSPPKPAVELDRHIDLDQAHAVASGGARIVLAPPARDRCRASEARLGAVIREARHVYGLTTGFGPLANRLISGENVRTLQANLVHHLASGVGPVLDWTTARAMVLARLVSIAQGASGASEGTIARLIDLLNSELAPAVPSRGTVGASGDLTPLAHMVLCLQGRGDFLDRDGTRLDGAEGLRRGRLQPLDLSHRDALALVNGTSAMTGIALVNAHACRHLGNWAVALTALLAECLRGRTEAWAAALSDLRPHPGQKDAAARLRARVDGSARVVRHVIAERRLDAGDIGTEPEAGQDAYSLRCAPQVLGAGFDTLAWHDRVLTIELNAVTDNPVFPPDGSVPALHGGNFMGQHVALTSDALATAVTVLAGLAERQIARLTDERLNRGLPPFLHRGPAGLNSGFMGAQVTATALLAEMRATGPASIHSISTNAANQDVVSLGTIAARLCREKIDRWAEILAILALCLAQAAELRCGSGLDGVSPAGKKLVQALREQFPPLETDRPLGQEIAALATHLLQQSPV huth-1 1545742 n/a 1545743 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 2O78; 1.90 A 2O7E; 1.75 A 2O7F; 2.00 A for 2078: R-factor: 0.172, R-free: 0.196. For 2O7E: R-factor: 0.189, R-free: 0.210. For 2O7F: R-factor: 0.172, R-free: 0.201 Fig 2a huth-1 1545742 n/a 1545743 unspecified role unspecified role neutral component neutral component Q3IWB0:p.His89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 2O6Y; 1.50 A 2O7B; 1.60 A 2O7D; 1.90 A For 2O6Y: R-factor: 0.174, R-free: 0.189. For 2O7B: R-factor: 0.192, R-free: 0.208. For 2O7D: R-factor: 0.175, R-free: 0.198. Fig. 2