UniProt UniProt http://www.uniprot.org braybrooke-2003-1 Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk J. Biol. Chem. (0021-9258) 2003 mimix curation braybrooke-2003-2 Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk J. Biol. Chem. (0021-9258) 2003 mimix curation braybrooke-2003-4 Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk J. Biol. Chem. (0021-9258) 2003 mimix curation braybrooke-2003-3 Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk J. Biol. Chem. (0021-9258) 2003 mimix curation braybrooke-2003-5 Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). ecoli Escherichia coli (strain K12) pull down pull down anti tag western anti tag western blot anti tag western Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk J. Biol. Chem. (0021-9258) 2003 mimix curation braybrooke-2003-6 Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). ecoli Escherichia coli (strain K12) affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight by staining molecular weight estimation by staining weight by staining Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk J. Biol. Chem. (0021-9258) 2003 mimix curation blm_human Bloom syndrome protein RecQ protein-like 3 DNA helicase, RecQ-like type 2 BLM RECQ2 RECQL3 protein protein human Homo sapiens Human MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS ra51d_human DNA repair protein RAD51 homolog 4 R51H3 RAD51-like protein 3 TRAD RAD51D RAD51L3 RAD51 homolog D protein protein human Homo sapiens Human MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITRDRDSGRLKPALGRSWSFVPSTRILLDTIEGAGASGGRRMACLAKSSRQPTGFQEMVDIGTWGTSEQSATLQGDQT xrcc2_human DNA repair protein XRCC2 XRCC2 X-ray repair cross-complementing protein 2 protein protein human Homo sapiens Human MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC rad51d-blm 1498916 n/a 1498922 unspecified role unspecified role prey prey n/a 1498923 unspecified role unspecified role bait bait physical association physical association false false false 1A and B rad51d-xrcc2-10 1498916 n/a 1498923 unspecified role unspecified role bait bait n/a 1498924 unspecified role unspecified role prey prey physical association physical association false false false 1A and B xrcc2-blm 1498917 n/a 1498922 unspecified role unspecified role prey prey n/a 1498924 unspecified role unspecified role bait bait physical association physical association false false false 1C rad51d-blm-1 1498917 n/a 1498922 unspecified role unspecified role prey prey n/a 1498923 unspecified role unspecified role bait bait physical association physical association false false false 1C rad51d-blm-4 1498918 n/a 1498922 unspecified role unspecified role prey prey n/a 1498923 unspecified role unspecified role bait bait direct interaction direct interaction false false false 2B rad51d-xrcc2-11 1498918 n/a 1498924 unspecified role unspecified role prey prey n/a 1498923 unspecified role unspecified role bait bait direct interaction direct interaction false false false 2B rad51d-blm-3 1498919 n/a 1498922 unspecified role unspecified role prey prey n/a 1498923 unspecified role unspecified role bait bait direct interaction direct interaction false false false 2A xrcc2-rad51d-3 1498920 n/a 1498923 unspecified role unspecified role prey prey n/a 1498924 unspecified role unspecified role bait bait physical association physical association false false false 3 xrcc2-rad51d-4 1498921 n/a 1498924 unspecified role unspecified role bait bait n/a 1498923 unspecified role unspecified role prey prey physical association physical association false false false 3