UniProt UniProt http://www.uniprot.org enouf-2003-1 Interactions of rotavirus VP4 spike protein with the endosomal protein Rab5 and the prenylated Rab acceptor PRA1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Enouf V., Chwetzoff S., Trugnan G., Cohen J. Specificity of the monoclonal antibody to Rab5 taken from supplier (Synaptic System) and confirmed by blasting immunogen. cohen@gv.cnrs-gif.fr CV-1 (African Green kidney fibroblast) cDNA J. Virol. (0022-538X) 2003 Only protein-protein interactions imex curation enouf-2003-4 Interactions of rotavirus VP4 spike protein with the endosomal protein Rab5 and the prenylated Rab acceptor PRA1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Enouf V., Chwetzoff S., Trugnan G., Cohen J. Specificity of the monoclonal antibody to Rab5 taken from supplier (Synaptic System) and confirmed by blasting immunogen. cohen@gv.cnrs-gif.fr CV-1 (African Green kidney fibroblast) cDNA J. Virol. (0022-538X) 2003 Only protein-protein interactions imex curation enouf-2003-3 Interactions of rotavirus VP4 spike protein with the endosomal protein Rab5 and the prenylated Rab acceptor PRA1. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Enouf V., Chwetzoff S., Trugnan G., Cohen J. COS7 cells transfected with Rab5a or Rab5c or RABAC1/PRA1have been infected with rotavirus Specificity of the monoclonal antibody to Rab5 taken from supplier (Synaptic System) and confirmed by blasting immunogen. cohen@gv.cnrs-gif.fr J. Virol. (0022-538X) 2003 Only protein-protein interactions imex curation enouf-2003-2 Interactions of rotavirus VP4 spike protein with the endosomal protein Rab5 and the prenylated Rab acceptor PRA1. chlae-ma104 Chlorocebus aethiops MA-104 kidney epithelial cells ma104 Green monkey MA-104 embryonic hidney epithelial cells Originally developed by initial explant culture from an embryonic rhesus monkey kidney cell line, however in 2002 it was discovered that the cells were contaminated with cells of African Green Monkey. The original Rhesus Monkey cells were completely overtaken by the AGMs between passage 7 and 12. http://www.lgcstandards-atcc.org/products/all/CRL-2378.1.aspx?geo_country=gb ATCC® CRL-2378.1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Enouf V., Chwetzoff S., Trugnan G., Cohen J. The "Fetal rhesus monkey kidney cell line MA104" as used in this publication - has been found to be contaminated with African Green Monkey cells; see pmid:4043530 MA104 cells (Green monkey) have been infected with bovine rotavirus RF strain Specificity of the monoclonal antibody to Rab5 taken from supplier (Synaptic System) and confirmed by blasting immunogen. cohen@gv.cnrs-gif.fr J. Virol. (0022-538X) 2003 Only protein-protein interactions imex curation rab5a_chlae_protein Green monkey Ras-related protein Rab-5A protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to human P20339 UniProt entry vp4_rotrf Outer capsid protein VP4 Hemagglutinin protein protein rotrf Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) MASLIYRQLLTNSYTVELSDEIQEIGSTKTQDVTVNPGPFAQTNYAPVNWGPGETNDSTTVEPVLDGPYQPTTFNPPVSYWMLLAPTNAGVVVEGTNNTNRWLATILIEPNVQQVERTYTLFGQQVQVTVSNNSQTKWKFVDLSKQTQDGNYSQHGSLLSTPKLYGVMKHGGKIYTYNGETPNATTDYYSTTNFDTVNMTAYCDFYIIPLAQEAKCTKYINNGLPPIQNTRNIVPVSIVSRNIVYTRAQPNQDIVVSKTSLWKEMQYNRDIVIRFKFANSIIKSGGLGYKWSEVSFKPANYQYTYTRDGEEVTAHTTCSVNGINNFNYNGGSLPTDFVISKYEVIKENSFVYIDYWDDSQAFRNMVNVRSLAADLNSVMCTGGDYSFALPLGHYPVMTGGAVSLHSAGVTLSTQFTDFVSLNSLRFRFRLSVEEPPFSILRTRVSGLYGLPAARPNNSQEYYEIAGRFSLISLVPSNDDYQTPIINSVTVRQDLERQLGELRDEFNNLSQQIAMSQLIDLALLPLDMFSMFSGIKSTIDAAKSMATNVMKRFKKSSLANSVSTLTDSLSDAASSISRNASVRSVSSTASAWTEVSNITSDINVTTSSISTQTSTISRRLRLKEMATQTDGMNFDDISAAVLKTKIDKSTQLNTNTLPEIVTEASEKFIPNRAYRVIKDDEVLEASTDGKYFAYKVETFEEIPFDVQKFADLVTDSPVISAIIDFKTLKNLNDNYGISRQQALNLLRSDPRVLREFINQDNPIIRNRIESLIMQCRL rabac1_chlae_protein Green monkey Prenylated Rab acceptor protein 1 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to human Q9UI14 UniProt entry. q96802-pro_0000368133 Outer capsid protein VP5* Hemagglutinin protein protein rotrf Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) AQPNQDIVVSKTSLWKEMQYNRDIVIRFKFANSIIKSGGLGYKWSEVSFKPANYQYTYTRDGEEVTAHTTCSVNGINNFNYNGGSLPTDFVISKYEVIKENSFVYIDYWDDSQAFRNMVNVRSLAADLNSVMCTGGDYSFALPLGHYPVMTGGAVSLHSAGVTLSTQFTDFVSLNSLRFRFRLSVEEPPFSILRTRVSGLYGLPAARPNNSQEYYEIAGRFSLISLVPSNDDYQTPIINSVTVRQDLERQLGELRDEFNNLSQQIAMSQLIDLALLPLDMFSMFSGIKSTIDAAKSMATNVMKRFKKSSLANSVSTLTDSLSDAASSISRNASVRSVSSTASAWTEVSNITSDINVTTSSISTQTSTISRRLRLKEMATQTDGMNFDDISAAVLKTKIDKSTQLNTNTLPEIVTEASEKFIPNRAYRVIKDDEVLEASTDGKYFAYKVETFEEIPFDVQKFADLVTDSPVISAIIDFKTLKNLNDNYGISRQQALNLLRSDPRVLREFINQDNPIIRNRIESLIMQCRL 248 776 rab5c_chlae_protein Green monkey Ras-related protein Rab-5C protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to human P51148 UniProt entry vp4-rab5a 1539812 n/a 1539816 unspecified role unspecified role prey prey n/a 1539817 unspecified role unspecified role bait bait physical association physical association false false false Table 1 vp4-rabac1 1539812 n/a 1539818 unspecified role unspecified role prey prey n/a 1539817 unspecified role unspecified role bait bait physical association physical association false false false Table 1 q96802_pro-rabac1 1539812 n/a 1539818 unspecified role unspecified role prey prey n/a 1539819 unspecified role unspecified role bait bait physical association physical association false false false Table 1 q96802_pro-rab5a 1539812 n/a 1539816 unspecified role unspecified role prey prey n/a 1539819 unspecified role unspecified role bait bait physical association physical association false false false Table 1 q96802_pro-rab5c 1539812 n/a 1539820 unspecified role unspecified role prey prey n/a 1539819 unspecified role unspecified role bait bait physical association physical association false false false Table 1 vp4-rabac1-1 1539813 n/a 1539818 unspecified role unspecified role prey prey n/a 1539817 unspecified role unspecified role bait bait physical association physical association false false false 5 q96802_pro-rabac1-1 1539813 n/a 1539818 unspecified role unspecified role prey prey n/a 1539819 unspecified role unspecified role bait bait Q96802-PRO_0000368133:p.Arg60_Gly62delinsIleAlaVal mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q96802-PRO_0000368133:p.[Pro148Asp;Gly153Asp] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false A VP5* construct 36-529 did not interact physical association physical association false false false 5 q96802_pro-rab5a-1 1539813 n/a 1539816 unspecified role unspecified role prey prey n/a 1539819 unspecified role unspecified role bait bait Q96802-PRO_0000368133:p.Arg60_Gly62delinsIleAlaVal mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q96802-PRO_0000368133:p.[Pro148Asp;Gly153Asp] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q96802-PRO_0000368133:p.[Phe258Ser;Leu261Val;Ile265Ser] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false A VP5* construct 36-529 did not interact physical association physical association false false false 5 vp4-rab5a-1 1539813 n/a 1539816 unspecified role unspecified role prey prey n/a 1539817 unspecified role unspecified role bait bait physical association physical association false false false 5 rab5a-vp4-1 1539814 n/a 1539816 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1539817 unspecified role unspecified role prey prey physical association physical association false false false 1B rab5c-vp4 1539814 n/a 1539820 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1539817 unspecified role unspecified role prey prey infection infection physical association physical association false false false 1B rabac1-vp4 1539814 n/a 1539818 unspecified role unspecified role bait bait infection infection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1539817 unspecified role unspecified role prey prey infection infection physical association physical association false false false 4 rab5a-vp4 1539815 n/a 1539816 unspecified role unspecified role bait bait cell lysate cell lysate n/a 1539817 unspecified role unspecified role prey prey infection infection physical association physical association false false false 1B and 2, 3 Interaction detected 6h after infection. Not detected 18h after infection (infection late stages)