IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fanto-2003-1 The tumor-suppressor and cell adhesion molecule Fat controls planar polarity via physical interactions with Atrophin, a transcriptional co-repressor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Fanto M., Clayton L., Meredith J., Hardiman K., Charroux B., Kerridge S., McNeill H. This paper describes a Y2H screen identifying 58 prey but only describes one. A list of many of the remaining prey is described in a subsequent paper, PMID: 25215488. The screen has therefore been added to that publication. Two baits were used, created from non-overlapping regions of the cytoplasmic domain of ft. There is no detail as to which prey bound to which bait fragment. helen.mcneill@cancer.org.uk Drosophila embryonic cDNA library Development (0950-1991) 2003 Only protein-protein interactions imex curation fanto-2003-2 The tumor-suppressor and cell adhesion molecule Fat controls planar polarity via physical interactions with Atrophin, a transcriptional co-repressor. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Fanto M., Clayton L., Meredith J., Hardiman K., Charroux B., Kerridge S., McNeill H. This paper describes a Y2H screen identifying 58 prey but only describes one. A list of many of the remaining prey is described in a subsequent paper, PMID: 25215488. The screen has therefore been added to that publication. helen.mcneill@cancer.org.uk Development (0950-1991) 2003 Only protein-protein interactions imex curation q8iqa6_drome Gug Atro Dmel\CG6964 GUG atro gug l(3)01323 l(3)03928 l(3)S146907 l(3)j5A3 l(3)rO116 Dmel_CG6964 CG6964 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFRELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMVRMPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQAAEFQRQSLHDQYFRQRPR fat_drome Cadherin-related tumor suppressor ft CG3352 Protein fat protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MERLLLLFFLLLAGRESLCQTGDTKLELLAPRGRSYATTYEQYAAFPRRRSSSSSPSGEMQSRAVDTSADFEVLEGQPRGTTVGFIPTKPKFSYRFNEPPREFTLDPVTGEVKTNVVLDREGMRDHYDLVVLSSQPTYPIEVRIKVLDVNDNSPEFPEPSIAISFSESATSGTRLLLDAATDADVGENGVTDQYEIVAGNVDNKFRLVTTANPSGDTSYLHLETTGNLDRESRGSYQLNISARDGGSPPRFGYLQVNVTILDVNDNPPIFDHSDYNVSLNETALPGTPVVTVMASDNDLGDNSKITYYLAETEHQFTVNPETGVISTTERVNCPQQTNVKSSASQKSCVFTVFARDHGSPRQDGRTYVTVNLLDTNDHDPIISFRFFPDGGKVATVDENAVNGTVVAAVAVKDSDSGLNGRTSVRIVSGNELGHFRLEEAADLHIVRVNGVLDREEIGKYNLTVVAMDQGTPARTTTAHLIIDVNDVNDHEPVFEKSEYSAVLSELAPTGSFVASITATDEDTGVNAQVHYDILSGNELKWFSMDPLTGLIVTTGPLDREIRDTVELSISARDGGPNPKFAYTQLKVIILDENDEAPQFSQREQNVTLGEDAPPQTIVALMTATDHDQGTNGSVTFALAPSVERLYPLQFALDALTGQLTTRRPLDREKMSQYEISVIARDQGAPTPQSATATVWLNVADVNDNDPQFYPRHYIYSLADDDDDIKLKKEVEKERILLHVTASDKDDGDNALIEYRLESGGEGLFQLDARSGAISLRGDAPASMHWKPHYKLLVSARDAGQRRSQQDAIVEIVLKSKLEMLECGQAQAGGYEFQMVEDHEQQRNSQPNREVGIVQVKSTNGKANSHIEYDIIQGDRAQNFRIDTRSGRITTARPLDREEQANYRLTILASSSSSSSAAASSVSYGQCIVNIAIIDLNDNAPVFALDRESEPTISLPENAAVGQEIYLSRVRDRDAGVNSRISYSLTNNPNQQFRIGPVTGVLYLQRPIRAEPGSLIHVELMATDAGSPPLSSKLSLSVLIADVNDHTPVFDHTSYETSLPETTKVNTRFFALAATDIDLGDNGRISYEIIEGNTERMFGVFPDGYLFVRAPLDREERDYYALTVSCRDAGQPSRSSVVPVVIHVIDENDNAPQFTNSTFTFSIPENAPADTFVGKLTAVDRDIGRNAELSFTLSSQTQDFTIDTRNGFIKTLRPFDREALVKVSRNAEASGEDGSLRGSMAGNYMLLEATVSDNGIPRLQDKVKVKVIVTDVNDNAPEFLRAPYHVTISEGASEGTHITHVFTQDADEGLNGDVYYSLAKGNEAGQFNLDSATGQLSLGRRLDRESQEIHHLIVVAKDAALKHPLSSNASITIVVLDENDNAPEFTQSSSEVSVLETSPTGTELMRFRASDADQGVNSQVVFSISAGNRRDTFHIDSITGSLYLHKPLDYEDITSYTLNITASDCGTPSLSTTVLYNVLVVDDNDNPPIFPSTAIVRQIKEGIPLKTPIVTVTADDPDSGLNGKVSYAISKQEPQLPQGRHFGINTETGVIHTLREIDRESIDTFRLTVVATDRAQPSERQLSTEKLVTVIVEDINDNAPVFVSMNAAILPPKFSTSKGSSTAVMQVHAKDADSSSNGLVTYEIVSGPQELFKLQRNTGIITFTPGPQFKQEVRYQLTLKSTDEAVQSERRSSEVYITIITPGSGGSESSVPQFEQRSKLSGSVYENEPIGTSILTVTAHLASAEIEYFVTNVTATGSRGQVDRLFDIDAKLGILSTAAELDREAGPEEYEVEVYAIALGGQPRTSRTKVRVTVLDKNDSPPQFLDTPFVYNVSEDLQIGHTISTLRAHDPDTLGSVTFLLMDGHDGKFLLEPSTGKLILNDTLDRETKSKYELRIRVSDGVQYTEAYATIQVSDTNDNPPLFEDTVYSFDIPENAQRGYQVGQIVARDADLGQNAQLSYGVVSDWANDVFSLNPQTGMLTLTARLDYEEVQHYILIVQAQDNGQPSLSTTITVYCNVLDLNDNAPIFDPMSYSSEVFENVPIATEVVTVSAKDIDSGNNGLIEYSITAGDVDSEFGIDSNGTIRTRRNLDREHRSTYTLTVTARDCADEFASFSELEETQLKLKYRSPRKYQQTRQEFLAHQKQQRLSSTVKVTILIKDVNDEVPVFISANETAIMENVAINTVVIAVKAVDNDEGRNGYIDYLMKEARDEDMGQSDPLPFSLNPTDGQLRVVDALDRELRSSYLLNITARDRGEPPQSTESQLLIRILDENDNSPVFDPKQYSASVAENASIGAMVLQVSATDVDEGANGRIRYSIVLGDQNHDFSISEDTGVVRVAKNLNYERLSRYSLTVRAEDCALENPAGDTAELTINILDINDNRPTFLDSPYLARVMENTVPPNGGYVLTVNAYDADTPPLNSQVRYFLKEGDSDLFRINASSGDIALLKPLDREQQSEYTLTLVAMDTGSPPLTGTGIVRVEVQDINDNDPVFELQSYHATVRENLPSGTHVLTPRATDKDEGLNAKLRFNLLGEHMHRFHIDSETGEISTATTLDREETSVYHLTLMAQDSSITEPRASSVNLTISVSDVNDNIPKFDSTTYNVAVPERISKGEFVFGARALDLDDGENAVVHYTISGRDQHYFDINTKTGVVSTKLELKTKTKSHDDLTYTIVISAMDQGEQSLSSKAELTVILRPPELFPTFAYMANSHFAMSEDVRPGKMITKVSATSPKKGLVGKIRYAIAGGIMGDSLRVDPNSGLLSVGQDGLDYELTHLYEIWIEAADGDTPSLRSVTLITLNVTDANDNAPVMEQLIYNAEVLEEESPPQLIAVVKASDRDSGDNGNVIYRLQNDFDGTFEITESGEIYTRMRLDREEIGDYAFVVEAVDQGVPHMTGTASVLLHLLDKNDNPPKFTRLFSLNVTENAEIGSFVIRVTSSDLDLGANANASYSFSENPGEKFRIEPQSGNITVAGHLDREQQDEYILKVVASDGAWRAETPITITIQDQNDNAPEFEHSFYSFSFPELQQSIALVGQIIATDRDKQGPNSVISYSLQQPSPMFSIDPATGEVFSKKAVRFKHSQYVRSPENMYALTVLATDNGKPPLYSECLVNINIVDAHNNPPKFEQAEYLAPLPQDAVRGQRIVRVHANDKQDLGTNEMDYSLMTFNLSSIFSVGRHDGWITLVKPIQVPPNTRYELVVRATDRGVPPQSDETRVVIVVTGENMDTPRFSVNSYQVIVPENEPVGSTILTVGATDDDTGPNGMLRYSISGGNERQDFSVDERTGGIVIQQQLDYDLIQEYHLNITVQDLGYHPLSSVAMLTIILTDVNDNPPVFNHKEYHCYIPENKPVGTFVFQAHAADKDSPKNAIIHYAFLPSGPDRHFFIMNQSNGTISSAVSFDYEERRIYTLQIKAKNPDSSMESYANLYVHVLGVNEFYPQFLQPVFHFDVSETSAVGTRVGAVQATDKDSGEDGRVYYLLVGSSNDKGFRIDTNTGLIYVARHLDRETQNRVVLTVMAKNYGSIRGNDTDEAQVIISIQDGNDPPEFIKHYYTSTISEAAPVGTKVTTVKAIDKDVRTQNNQFSYSIINGNLKQSFKIDVQTGEISTASRLDREETSTYNLVIGAIDTGLPPQTGSATVHIELEDVNDNGPTFTPEGLNGYISENEPAGTSIMTLIASDPDLPRNGGPFTYQLIGGKHKSWLSVDRNSGVVRSTTSFDREMTPILEAIIEVEDSGKPKQKSQHLLTITVLDQNDNPSTTRSLHIAVSLFNGDLPSNVKLADVRPNDIDIVGDYRCRLQKNPAQSQLQLAIPRACDLITTSHTTPIASVFSYTGNDGKHGDVSSKVSVAFQSFNNETLANSVSIMVRNMTAYHFLANHYRPILEMIKSRMSNEDEVILYSLLEGGSGNSTNLQLLMAVRLAKTSYQQPKYLIERLREKRSAFSELLQKEVIVGYEPCSEPDVCENGGVCSATMRLLDAHSFVIQDSPALVLSGPRVVHDYSCQCTSGFSGEQCSRRQDPCLPNPCHSQVQCRRLGSDFQCMCPANRDGKHCEKERSDVCYSKPCRNGGSCQRSPDGSSYFCLCRPGFRGNQCESVSDSCRPNPCLHGGLCVSLKPGYKCNCTPGRYGRHCERFSYGFQPLSYMTFPALDVTTNDISIVFATTKPNSLLLYNYGMQSGGRSDFLAIELVHGRAYFSSGGARTAISTVIAGRNLADGGWHKVTATRNGRVMSLSVAKCADSGDVCTECLPGDSSCYADEVGPVGTLNFNKQPLMIGGLSSADPILERPGQVHSDDLVGCLHSVHIGGRALNLSLPLQQKGILAGCNRQACQPALAAERCGGFAGQCIDRWSSSLCQCGGHLQSPDCSDSLEPITLGEGAFVEFRISEIYRRMQLLDNLYNSKSAWLDNQQMRERRAVSNFSTASQIYEAPKMLSMLFRTYKDQGQILYAATNQMFTSLSLREGRLVYYSKQHLTINMTVQETSTLNDGKWHNVSLFSESRSLRLIVDGRQVGDELDIAGVHDFLDPYLTILNVGGEAFVGCLANVTVNNELQPLNGSGSIFPEVRYHGKIESGCRGDIGQDAAQVADPLSIGFTLVIVFFVILVVAILGSYVIYRFRGKQEKIGSLSCGVPGFKIKHPGGPVTQSQVDHVLVRNLHPSEAPSPPVGAGDHMRPPVGSHHLVGPELLTKKFKEPTAEMPQPQQQQQRPQRPDIIERESPLIREDHHLPIPPLHPLPLEHASSVDMGSEYPEHYDLENASSIAPSDIDIVYHYKGYREAAGLRKYKASVPPVSAYTHHKHQNSGSQQQQQQHRHTAPFVTRNQGGQPPPPPTSASRTHQSTPLARLSPSSELSSQQPRILTLHDISGKPLQSALLATTSSSGGVGKDVHSNSERSLNSPVMSQLSGQSSSASRQKPGVPQQQAQQTSMGLTAEEIERLNGRPRTCSLISTLDAVSSSSEAPRVSSSALHMSLGGDVDAHSSTSTDESGNDSFTCSEIEYDNNSLSGDGKYSTSKSLLDGRSPVSRALSGGETSRNPPTTVVKTPPIPPHAYDGFESSFRGSLSTLVASDDDIANHLSGIYRKANGAASPSATTLGWEYLLNWGPSYENLMGVFKDIAELPDTNGPSQQQQQQTQVVSTLRMPSSNGPAAPEEYV ft-gug-2 1762952 n/a 1762954 unspecified role unspecified role prey prey n/a 1762955 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false gug-ft 1762953 n/a 1762954 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1762955 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro direct interaction direct interaction false false false Fig.2B