UniProt UniProt http://www.uniprot.org baumann-2000-3 The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Baumann M., Gires O., Kolch W., Mischak H., Zeidler R., Pich D., Hammerschmidt W. hammerschmidt@gsf.de EBV-immortalized lymphoblastoid cell line cDNA Eur. J. Biochem. (0014-2956) 2000 imex curation Only protein-protein interactions baumann-2000-5 The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Baumann M., Gires O., Kolch W., Mischak H., Zeidler R., Pich D., Hammerschmidt W. hammerschmidt@gsf.de Eur. J. Biochem. (0014-2956) 2000 imex curation Only protein-protein interactions baumann-2000-1 The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Baumann M., Gires O., Kolch W., Mischak H., Zeidler R., Pich D., Hammerschmidt W. hammerschmidt@gsf.de EBV-immortalized lymphoblastoid cell line cDNA Eur. J. Biochem. (0014-2956) 2000 imex curation Only protein-protein interactions baumann-2000-2 The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Baumann M., Gires O., Kolch W., Mischak H., Zeidler R., Pich D., Hammerschmidt W. hammerschmidt@gsf.de EBV-immortalized lymphoblastoid cell line cDNA Eur. J. Biochem. (0014-2956) 2000 imex curation Only protein-protein interactions baumann-2000-4 The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Baumann M., Gires O., Kolch W., Mischak H., Zeidler R., Pich D., Hammerschmidt W. hammerschmidt@gsf.de Eur. J. Biochem. (0014-2956) 2000 imex curation Only protein-protein interactions bzlf1_ebvb9 Trans-activator protein BZLF1 Zebra BZLF1 protein protein ebvb9 Epstein-Barr virus (strain B95-8) (HHV-4) Human herpesvirus 4 HHV-4 MMDPNSTSEDVKFTPDPYQVPFVQAFDQATRVYQDLGGPSQAPLPCVLWPVLPEPLPQGQLTAYHVSTAPTGSWFSAPQPAPENAYQAYAAPQLFPVSDITQNQQTNQAGGEAPQPGDNSTVQTAAAVVFACPGANQGQQLADIGVPQPAPVAAPARRTRKPQQPESLEECDSELEIKRYKNRVASRKCRAKFKQLLQHYREVAAAKSSENDRLRLLLKQMCPSLDVDSIIPRTPDVLHEDLLNF rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR kpca_human Protein kinase C alpha type PRKCA PKCA PRKACA protein protein human Homo sapiens Human MADVFPGNDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSVCKGLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV bzlf1-gnb2l1-2 1532815 n/a 1532820 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1532821 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association false false false 3A gnb2l1-bzlf1 1532815 n/a 1532820 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1532821 unspecified role unspecified role bait bait n-terminal region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 3A bzlf1-prkca-1 1532816 n/a 1532822 enzyme enzyme neutral component neutral component purified purified n/a 1532820 enzyme target enzyme target substrate substrate neutral component neutral component purified purified ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 5A GNB2L1/RACK1 did not influence phosphorylation prkca-prkca 1532816 n/a 1532822 enzyme target enzyme target substrate substrate neutral component neutral component purified purified n/a 1532822 enzyme enzyme neutral component neutral component purified purified phosphorylation phosphorylation reaction phosphorylation false false false 5A GNB2L1/RACK1 did not influence phosphorylation bzlf1-gnb2l1 1532817 n/a 1532820 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1532821 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure bzlf1-gnb2l1-1 1532818 n/a 1532821 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1532820 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1C gnb2l1-bzlf1-2 1532819 n/a 1532820 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1532821 unspecified role unspecified role bait bait physical association physical association false false false 3B and C Incubation under stringent conditions in RIPA buffer completely abolished interaction; increasing the salt concentration to 500 mm NaCl severely impaired interaction; stimulation with TPA did not change interactions.