bhf-ucl li-1997-1 The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu J. Biol. Chem. (0021-9258) 1997 imex curation Only protein-protein interactions li-1997-3 The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu J. Biol. Chem. (0021-9258) 1997 imex curation Only protein-protein interactions li-1997-2 The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu J. Biol. Chem. (0021-9258) 1997 imex curation Only protein-protein interactions kcnh2_human Potassium voltage-gated channel subfamily H member 2 Voltage-gated potassium channel subunit Kv11.1 Ether-a-go-go-related gene potassium channel 1 Eag homolog KCNH2 ERG ERG1 HERG protein protein human Homo sapiens Human MPVRRGHVAPQNTFLDTIIRKFEGQSRKFIIANARVENCAVIYCNDGFCELCGYSRAEVMQRPCTCDFLHGPRTQRRAAAQIAQALLGAEERKVEIAFYRKDGSCFLCLVDVVPVKNEDGAVIMFILNFEVVMEKDMVGSPAHDTNHRGPPTSWLAPGRAKTFRLKLPALLALTARESSVRSGGAGGAGAPGAVVVDVDLTPAAPSSESLALDEVTAMDNHVAGLGPAEERRALVGPGSPPRSAPGQLPSPRAHSLNPDASGSSCSLARTRSRESCASVRRASSADDIEAMRAGVLPPPPRHASTGAMHPLRSGLLNSTSDSDLVRYRTISKIPQITLNFVDLKGDPFLASPTSDREIIAPKIKERTHNVTEKVTQVLSLGADVLPEYKLQAPRIHRWTILHYSPFKAVWDWLILLLVIYTAVFTPYSAAFLLKETEEGPPATECGYACQPLAVVDLIVDIMFIVDILINFRTTYVNANEEVVSHPGRIAVHYFKGWFLIDMVAAIPFDLLIFGSGSEELIGLLKTARLLRLVRVARKLDRYSEYGAAVLFLLMCTFALIAHWLACIWYAIGNMEQPHMDSRIGWLHNLGDQIGKPYNSSGLGGPSIKDKYVTALYFTFSSLTSVGFGNVSPNTNSEKIFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVREFIRFHQIPNPLRQRLEEYFQHAWSYTNGIDMNAVLKGFPECLQADICLHLNRSLLQHCKPFRGATKGCLRALAMKFKTTHAPPGDTLVHAGDLLTALYFISRGSIEILRGDVVVAILGKNDIFGEPLNLYARPGKSNGDVRALTYCDLHKIHRDDLLEVLDMYPEFSDHFWSSLEITFNLRDTNMIPGSPGSTELEGGFSRQRKRKLSFRRRTDKDTEQPGEVSALGPGRAGAGPSSRGRPGGPWGESPSSGPSSPESSEDEGPGRSSSPLRLVPFSSPRPPGEPPGGEPLMEDCEKSSDTCNPLSGAFSGVSNIFSFWGDSRGRQYQELPRCPAPTPSLLNIPLSSPGRRPRGDVESRLDALQRQLNRLETRLSADMATVLQLLQRQMTLVPPAYSAVTTPGPGPTSTSPLLPVSPLPTLTLDSLSQVSQFMACEELPPGAPELPQEGPTRRLSLPGQLGALTSQPLHRHGSDPGS kcnh2-kcnh2 1426967 n/a HERG 1426970 unspecified role unspecified role unspecified role unspecified role purified purified range gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 2A kcnh2-1 1426967 n/a HERG 1426970 unspecified role unspecified role unspecified role unspecified role purified purified n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false range gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2A kcnh2-1 1426968 n/a HERG 1426970 unspecified role unspecified role unspecified role unspecified role purified purified n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2B kcnh2-1 1426968 n/a HERG 1426970 unspecified role unspecified role unspecified role unspecified role n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 2B kcnh2-kcnh2-3 1426969 n/a HERG 1426970 unspecified role unspecified role unspecified role unspecified role purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false ecolx Escherichia coli n/a 1426970 unspecified role unspecified role unspecified role unspecified role purified purified n-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli 1426990 1426988 direct interaction direct interaction false false false 1B