bhf-ucl battey-1994-1 The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting polyclonal western polyclonal antibody western blot polyclonal western Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. J. Biol. Chem. (0021-9258) 1994 imex curation The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. Only protein-protein interactions battey-1994-2 The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom polyclonal western polyclonal antibody western blot polyclonal western Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. J. Biol. Chem. (0021-9258) 1994 imex curation The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. Only protein-protein interactions battey-1994-3 The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. Interactions with VLDL could not be captured as the origin of this protein was not given. J. Biol. Chem. (0021-9258) 1994 imex curation The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. Only protein-protein interactions battey-1994-4 The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. J. Biol. Chem. (0021-9258) 1994 imex curation The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. Only protein-protein interactions amrp_human Alpha-2-macroglobulin receptor-associated protein Low density lipoprotein receptor-related protein-associated protein 1 LRPAP1 A2MRAP protein protein human Homo sapiens Human MAPRRVRSFLRGLPALLLLLLFLGPWPAASHGGKYSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL vldlr_human Very low-density lipoprotein receptor VLDLR protein protein human Homo sapiens Human MGTSALWALWLLLALCWAPRESGATGTGRKAKCEPSQFQCTNGRCITLLWKCDGDEDCVDGSDEKNCVKKTCAESDFVCNNGQCVPSRWKCDGDPDCEDGSDESPEQCHMRTCRIHEISCGAHSTQCIPVSWRCDGENDCDSGEDEENCGNITCSPDEFTCSSGRCISRNFVCNGQDDCSDGSDELDCAPPTCGAHEFQCSTSSCIPISWVCDDDADCSDQSDESLEQCGRQPVIHTKCPASEIQCGSGECIHKKWRCDGDPDCKDGSDEVNCPSRTCRPDQFECEDGSCIHGSRQCNGIRDCVDGSDEVNCKNVNQCLGPGKFKCRSGECIDISKVCNQEQDCRDWSDEPLKECHINECLVNNGGCSHICKDLVIGYECDCAAGFELIDRKTCGDIDECQNPGICSQICINLKGGYKCECSRGYQMDLATGVCKAVGKEPSLIFTNRRDIRKIGLERKEYIQLVEQLRNTVALDADIAAQKLFWADLSQKAIFSASIDDKVGRHVKMIDNVYNPAAIAVDWVYKTIYWTDAASKTISVATLDGTKRKFLFNSDLREPASIAVDPLSGFVYWSDWGEPAKIEKAGMNGFDRRPLVTADIQWPNGITLDLIKSRLYWLDSKLHMLSSVDLNGQDRRIVLKSLEFLAHPLALTIFEDRVYWIDGENEAVYGANKFTGSELATLVNNLNDAQDIIVYHELVQPSGKNWCEEDMENGGCEYLCLPAPQINDHSPKYTCSCPSGYNVEENGRDCQSTATTVTYSETKDTNTTEISATSGLVPGGINVTTAVSEVSVPPKGTSAAWAILPLLLLVMAAVGGYLMWRNWQHKNMKSMNFDNPVYLKTTEEDLSIDIGRHSASVGHTYPAISVVSTDDDLA lrpap1-vldlr-2 1463730 n/a 1463734 unspecified role unspecified role bait bait purified purified escherichia coli dh5[alpha] escherichia coli dh5[alpha] n/a 1463735 unspecified role unspecified role prey prey cell lysate cell lysate crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Figures 2 A, B, C and D lrpap1-vldlr-6 1463731 n/a 1463734 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified escherichia coli dh5[alpha] escherichia coli dh5[alpha] n/a 1463735 unspecified role unspecified role prey prey cell lysate cell lysate crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Figure 3 C vldlr-lrpap1 1463732 n/a 1463735 unspecified role unspecified role bait bait partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. n/a 1463734 unspecified role unspecified role prey prey purified purified escherichia coli dh5[alpha] escherichia coli dh5[alpha] direct interaction direct interaction false false false 1463732 Figure 4 A lrpap1-vldlr-5 1463733 n/a 1463734 unspecified role unspecified role bait bait purified purified 125i radiolabel 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false escherichia coli dh5[alpha] escherichia coli dh5[alpha] n/a 1463735 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Figure 3 A