IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fan-2013-1 EGFR Phosphorylates Tumor-Derived EGFRvIII Driving STAT3/5 and Progression in Glioblastoma. human-glioblastoma Homo sapiens glioblastoma glioblastoma Glioblastoma Glioblastoma is the same as 'Glioblastoma multiforme' (GBM) -is the same as Astrocytoma Grade 4. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Fan Q.W., Cheng C.K., Gustafson W.C., Charron E., Zipper P., Wong R.A., Chen J., Lau J., Knobbe-Thomsen C., Weller M., Jura N., Reifenberger G., Shokat K.M., Weiss W.A. qiwen.fan@ucsf.edu,waweiss@gmail.com Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation fan-2013-2 EGFR Phosphorylates Tumor-Derived EGFRvIII Driving STAT3/5 and Progression in Glioblastoma. human-ln_229 Homo sapiens malignant glioma cell line LN-229 ln_229 Human malignant glioma cell line LN-229 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fan Q.W., Cheng C.K., Gustafson W.C., Charron E., Zipper P., Wong R.A., Chen J., Lau J., Knobbe-Thomsen C., Weller M., Jura N., Reifenberger G., Shokat K.M., Weiss W.A. qiwen.fan@ucsf.edu,waweiss@gmail.com Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation fan-2013-4 EGFR Phosphorylates Tumor-Derived EGFRvIII Driving STAT3/5 and Progression in Glioblastoma. human-glioblastoma Homo sapiens glioblastoma glioblastoma Glioblastoma Glioblastoma is the same as 'Glioblastoma multiforme' (GBM) -is the same as Astrocytoma Grade 4. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Fan Q.W., Cheng C.K., Gustafson W.C., Charron E., Zipper P., Wong R.A., Chen J., Lau J., Knobbe-Thomsen C., Weller M., Jura N., Reifenberger G., Shokat K.M., Weiss W.A. qiwen.fan@ucsf.edu,waweiss@gmail.com Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation fan-2013-3 EGFR Phosphorylates Tumor-Derived EGFRvIII Driving STAT3/5 and Progression in Glioblastoma. human-ln_229 Homo sapiens malignant glioma cell line LN-229 ln_229 Human malignant glioma cell line LN-229 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Fan Q.W., Cheng C.K., Gustafson W.C., Charron E., Zipper P., Wong R.A., Chen J., Lau J., Knobbe-Thomsen C., Weller M., Jura N., Reifenberger G., Shokat K.M., Weiss W.A. qiwen.fan@ucsf.edu,waweiss@gmail.com Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D egfr-egfr-13 1456088 n/a 1456092 unspecified role unspecified role neutral component neutral component egfrviii sufficient to bind sufficient binding region sufficient to bind range range range range false range range range range false n/a 1456092 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1C EGFR and EGFRvIII colocalized in tumor cells (Figure 1C). egfr-egfr-14 1456088 n/a 1456092 unspecified role unspecified role neutral component neutral component egfrviii sufficient to bind sufficient binding region sufficient to bind range range range range false range range range range false n/a 1456093 unspecified role unspecified role neutral component neutral component possible_phospho_705tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 3Di egfr-egfr-15 1456088 n/a 1456093 unspecified role unspecified role neutral component neutral component possible_phospho_705tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false n/a 1456092 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3Dii stat3-egfr 1456089 n/a 1456093 unspecified role unspecified role bait bait n/a 1456092 unspecified role unspecified role prey prey physical association physical association false false false 7Ci EGF stat3-egfr-1 1456089 n/a 1456092 unspecified role unspecified role prey prey n/a 1456093 unspecified role unspecified role bait bait n/a 1456092 unspecified role unspecified role prey prey egfrviii sufficient to bind sufficient binding region sufficient to bind range range range range false range range range range false association association false false false 7Cii EGF stat3-egfr-2 1456089 n/a 1456093 unspecified role unspecified role bait bait n/a 1456092 unspecified role unspecified role prey prey egfrviii sufficient to bind sufficient binding region sufficient to bind range range range range false range range range range false physical association physical association false false false 7Ciii A weak reaction with added EGF having little effect. egfr-egfr-17 1456090 n/a 1456093 unspecified role unspecified role neutral component neutral component possible_phospho_705tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false n/a 1456092 unspecified role unspecified role neutral component neutral component egfrviii sufficient to bind sufficient binding region sufficient to bind range range range range false range range range range false colocalization colocalization false false false 3Di egfr-egfr-17 1456090 n/a 1456092 unspecified role unspecified role neutral component neutral component n/a 1456093 unspecified role unspecified role neutral component neutral component possible_phospho_705tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 3Dii egfr-egfr-16 1456091 n/a 1456092 unspecified role unspecified role neutral component neutral component egfrviii sufficient to bind sufficient binding region sufficient to bind range range range range false range range range range false n/a 1456092 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp Figure 1G