IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ stinson-2013-3 Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation stinson-2013-1 Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation stinson-2013-6 Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation stinson-2013-7 Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation stinson-2013-4 Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation stinson-2013-5 Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation stinson-2013-2 Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. in vitro In vitro fluorescence fluorescence technology fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation clpx_ecoli ATP-dependent Clp protease ATP-binding subunit ClpX clpX lopC b0438 JW0428 ATP-dependent unfoldase ClpX protein protein ecoli Escherichia coli (strain K12) MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLCNDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKAKSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALTKQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMEDVEKVVIDESVIDGQSKPLLIYGKPEAQQASGE atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adenosine 5'-[gamma-thio]triphosphate Adenosine 5'-[gamma-thio]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) atp-clpx-1 1432769 n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432777 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.[Val78_Ile79delinsAlaAla;Glu185Gln] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 1432786 1432783 atpase reaction atpase reaction false false false Fig. 2D, Supp Fig. 1B, 3B Either Mg2+ or Co2+ required for function atp-clpx-2 1432769 n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 n/a 1432777 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) atpase reaction atpase reaction false false false Supp. Fig. 1B adenosine_5__-clpx-1 1432769 n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432778 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 1432803 1432797 atpase reaction atpase reaction false false false Fig. 2E, Supp. figure 3C atp-clpx-3 1432769 n/a 1432777 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 atpase reaction atpase reaction false false false Fig.6C A dimer comprised of ClpX lacking a stretch of N-terminal sequence, was only 75% active, a glu185gln mutation made this construct totally inactive. atp-clpx-6 1432769 n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Lys330Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false cys-330 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432777 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 atpase reaction atpase reaction false false false Supp Fig. 4A Binding of a Oregon Green dye to a met363cys mutated residue in one subunit did not affect enzyme function atp-clpx-7 1432769 n/a 1432777 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 enzyme enzyme neutral component neutral component cys-363 oregon green label oregon green label certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 atpase reaction atpase reaction false false false Supp Fig. 3E Binding of a Oregon Green dye to a met363cys mutated residue in one subunit did not affect enzyme function atp-clpx-8 1432769 n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Glu205Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Thr147Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1432777 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 1432840 1432836 atpase reaction atpase reaction false false false Fig. 5C Either Mg2+ or Co2+ required for function atp-clpx-9 1432769 n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Thr147Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432777 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Glu205Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 1432851 1432844 atpase reaction atpase reaction false false false Fig. 5C Either Mg2+ or Co2+ required for function clpx-1 1432770 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Supp. Table 1 4.1A (4I34) 4.5A (4I5O) 5.7A (4I63) 4.5A (4I9K) Working/free 27.4/30.8 (4I34) 28.6/32.3 (4I5O) 30.7/31.5 (4I63) 32.2/35.2 (4I9K) adenosine_5___g-clpx 1432770 n/a 1432778 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Supp. Table 1 3.8A Working/free 26.2/29.6 adp-clpx 1432770 n/a 1432779 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Supp. Table 1 3.7A Working/free 27.5/33.4 (4I4L) adp-clpx-2 1432771 n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432779 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Supp Fig. 3F, 3G Binding to the rightmost wild-type subunit subunit was tight (Kapp 2-14 uM), binding to the leftmost wild-type subunit was weaker (Kapp 60-90 uM), and binding to the VI subunit was even weaker (Kapp 430 uM) or undetectable. atp-clpx-4 1432771 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432777 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 3C, Supp Fig. 3G Binding to the rightmost wild-type subunit subunit was tight (Kapp 2-14 uM), binding to the leftmost wild-type subunit was weaker (Kapp 60-90 uM), and binding to the VI subunit was even weaker (Kapp 430 uM) or undetectable adenosine_5__-clpx-2 1432771 n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432778 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432776 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 3B, Supp fig. 3G Binding to the rightmost wild-type subunit subunit was tight (Kapp 2-14 uM), binding to the leftmost wild-type subunit was weaker (Kapp 60-90 uM), and binding to the VI subunit was even weaker (Kapp 430 uM) or undetectable adp-clpx-3 1432772 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432779 unspecified role unspecified role neutral component neutral component n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Supp Fig. 4C, Supp 4D The first nucleotide-binding events cause allosteric changes in both bound (rightmost) and unbound (leftmost) subunits of the ring, possibly altering the hinge conformations and rigidifying the domain-domain interfaces in nucleotide loadable subunits. adenosine_5__-clpx-3 1432772 n/a 1432778 unspecified role unspecified role neutral component neutral component n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432776 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4B, Supp Fig. 4D The first nucleotide-binding events cause allosteric changes in both bound (rightmost) and unbound (leftmost) subunits of the ring, possibly altering the hinge conformations and rigidifying the domain-domain interfaces in nucleotide loadable subunits. atp-clpx-5 1432772 n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432777 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false Fig. 4C, Supp. Fig. 4D The first nucleotide-binding events cause allosteric changes in both bound (rightmost) and unbound (leftmost) subunits of the ring, possibly altering the hinge conformations and rigidifying the domain-domain interfaces in nucleotide loadable subunits. 10 uM cp7-CFP-ssrA was present in the assay mix. adp-clpx 1432773 n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 n/a 1432779 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1432773 Supp. Fig. 2B adp-clpx-1 1432773 n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1432779 unspecified role unspecified role neutral component neutral component n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 1432773 Supp Fig. 2A clpx-2 1432774 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Fig. 6B, Supp. Fig. 2C Removal of a stretch of N-terminal sequence reduced this to a dimeric interaction. clpx-3 1432774 n/a 1432776 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp Fig. 2C clpx-1 1432775 n/a 1432776 unspecified role unspecified role neutral component neutral component cys-76 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Lys330Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false cys-330 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Asp76Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 n/a 1432776 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cys-330 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false cys-76 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Lys330Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Asp76Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 1432955 1432949 direct interaction direct interaction false false false Fig. 1D