UniProt UniProt http://www.uniprot.org gladue-2013-2 Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Gladue D.P., O'Donnell V., Baker-Branstetter R., Holinka L.G., Pacheco J.M., Fernández Sainz I., Lu Z., Ambroggio X., Rodriguez L., Borca M.V. Cells have been infected with FMDV manuel.borca@ars.usda.gov pmanuel.borca@ars.usda.gov J. Virol. (0022-538X) 2013 Only protein-protein interactions imex curation gladue-2013-1 Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Gladue D.P., O'Donnell V., Baker-Branstetter R., Holinka L.G., Pacheco J.M., Fernández Sainz I., Lu Z., Ambroggio X., Rodriguez L., Borca M.V. Cells have been infected with FMDV manuel.borca@ars.usda.gov pmanuel.borca@ars.usda.gov bovine cDNA from FMDV-susceptible tissues (dorsal soft palate, interdigital skin, middle tongue epithelium and middle anterior lung) J. Virol. (0022-538X) 2013 Only protein-protein interactions imex curation gladue-2013-3 Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival. human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gladue D.P., O'Donnell V., Baker-Branstetter R., Holinka L.G., Pacheco J.M., Fernández Sainz I., Lu Z., Ambroggio X., Rodriguez L., Borca M.V. Cells have been infected with FMDV manuel.borca@ars.usda.gov pmanuel.borca@ars.usda.gov J. Virol. (0022-538X) 2013 Only protein-protein interactions imex curation gladue-2013-4 Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival. human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Gladue D.P., O'Donnell V., Baker-Branstetter R., Holinka L.G., Pacheco J.M., Fernández Sainz I., Lu Z., Ambroggio X., Rodriguez L., Borca M.V. Cells have been infected with FMDV manuel.borca@ars.usda.gov pmanuel.borca@ars.usda.gov J. Virol. (0022-538X) 2013 Only protein-protein interactions imex curation q8uzc1_9pico Capsid protein VP0 Capsid protein VP1 Capsid protein VP3 Capsid protein VP4 Genome polyprotein Genome-linked protein VPg1 Genome-linked protein VPg2 Genome-linked protein VPg3 Leader protease P1A P1B P1C P1D P52 P56A Picornain 3C Protease 3C Protease P20B Protein 2A Protein 2C Protein 3A Protein 3B-1 Protein 3B-2 Protein 3B-3 RNA-directed RNA polymerase 3D-POL VP4-VP2 Virion protein 1 Virion protein 2 Virion protein 3 Virion protein 4 protein 2B protein protein foot-and-mouth disease virus - type o Foot-and-mouth disease virus - type O MNTTDCFIALVQAIREIKALFLPRTTGKMELTPYNGEKKTFYSRPNNHDNCWLNAILQLFRYVEEPFFDWVYSSPENLTLEAIKQLEDLTGLELHEGGPPALVIWNIKHLLHTGIGTASRPSEVCMVDGTDMCLADFHAGIFLKGQEHAVFACVTSNGWYAIDDEDFYPWTPDPSDVLVFVPYDQEPLNGEWKAKVQRKLKGAGQSSPATGSQNQSGNTGSIINNYYMQQYQNSMDTQLGDNAISGGSNEGSTDTTSTHTTNTQNNDWFSKLASSAFSGLFGALLADKKTEETTLLEDRILTTRNGHTTSTTQSSVGVTYGYATAEDFVSGPNTSGLETRVVQAERFFKTHLFDWVTSDSFGRCHLLELPTDHKGVYGSLTDSYAYMRNGWDVEVTAVGNQFNGGCLLVAMVPELCSIQKRELYQLTLFPHQFINPRTNMTAHITVPFVGVNRYDQYKVHKPWTLVVMVVAPLTVNTEGAPQIKVYANIAPTNVHVAGEFPSKEGIFPVACSDGYGGLVTTDPKTADPVYGKVFNPPRNQLPGRFTNLLDVAEACPTFLRFEGGVPYVTTKTDSDRVLAQFDMSLAAKHMSNTFLAGLAQYYTQYSGTINPHFMFTGPTDAKARYMIAYAPPGMEPPKTPEAAAHCIHAEWDTGLNSKFTFSIPYLSAADYAYTASDVAETTNVQGWVCLFQITHGKADGDALVVLASAGKDFELRLPVDARAETTSAGESADPVTTTVENYGGETQIQRRQHTDVSFIMDRFVKVTPQNQINILDLMQIPSHTLVGALLRASTYYFSDLEIAVKHEGDLTWVPNGAPEKGLDNTTNPTAYHKAPLTRLALPYTAPHRVLATVYNGECRYSRNAVPNVRGDLQVLAQKVVRTLPTSFNYGAIKATRVTELLYRMKRAETYCPRPLLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGPFFFSDVRSNFSKLVETINQMQEDMSTKHGPDFNRLVSAFEELAIGVKAIRTGLDEAKPWYKLIKLLSRLSCMAAVAARSKDPVLVAIMLADTGLEILDSTFVVKKISDSLSSLFHVPAPVFSFGAPILLAGLVKVASSFFRSTPEDLERAEKQLKARDINDIFAILKNGEWLVKLILAIRDWIKAWIASEEKFVTMTDLVPGILEKQRDLNDPSKYKEAKEWLDNARQACLKSGNVHIANLCKVVAPAPSKSRPEPVVVCLRGKSGQGKSFLANVLAQAISAHFTGRTDSVWYCPPDPDHFDGYNQQTVVVMDDLGQNPDGKDFKYFAQMVSTTGFIPPMASLEDKGKPFNSKVIIATTNLYSGFTPRTMVCPDALNRRFHFDIDVSAKDGYKINNKLDIIKALEDTHTNPVAMFQYDCALLNGMAVEMKRMQQDMFKPQPPLQNVYQLVQEVIDRVELHEKVSSHPIFKQISIPSQKSVLYFLIEKGQHEAAIEFFEGMVHDSIKEELRPLIQQTSFVKRAFKRLKENFEIVALCLTLLANIVIMIRETRKRQKMVDDAVNEYIEKANITTDDKTLDEAEKSPLETSGASTVGFRERTLPGQKACDDVNSEPAQPVEEQPQAEGPYAGPLERQKPLKVRAKLPQQEGPYAGPMERQKPLKVKAKAPVVKEGPYEGPVKKPVALKVKAKNLIVTESGAPPTDLQKMVMGNTKPVELILDGKTVAICCATGVFGTAYLVPRHLFAEKYDKIMLDGRAMTDSDYRVFEFEIKVKGQDMLSDAALMVLHRGNRVRDITKHFRDTARMKKGTPVVGVINNADVGRLIFSGEALTYKDIVVCMDGDTMPGLFAYRAATKAGYCGGAVLAKDGADTFIVGTHSAGGNGVGYCSCVSRSMLLKMKAHIDPEPHHEGLIVDTRDVEERVHVMRKTKLAPTVAHGVFNPEFGPAALSNKDPRLNEGVVLDEVIFSKHKGDTKMSEEDKALFRRCAADYASRLHSVLGTANAPLSIYEAIKGVDGLDAMEPDTAPGLPWALQGKRRGALIDFENGTVGPEVEAALKLMEKREYKFACQTFLKDEIRPMEKVRAGKTRIVDVLPVEHILYTRMMIGRFCAQMHSNNGPQIGSAVGCNPDVDWQRFGTHFAQYRNVWDVDYSAFDANHCSDAMNIMFEEVFRTEFGFHPNAEWILKTLANTEHAYENKRITVEGGMPSGCSATSIINTILNNIYVLYALRRHYEGVELDTYTMISYGDDIVVASDYDLDFEALKPHFKSLGQTITPADKSDKGFVLGHSITDVTFLKRHFHMDYGTGFYKPVMASKTLEAILSFARRGTIQEKLISVAGLAVHSGPDEYRRLFEPFQGLFEIPSYRSLYLRWVNAVCGDA vime_bovin Vimentin VIM protein protein bovin Bos taurus (Bovine) Bovine MSTRSVSSSSYRRMFGGPGTASRPSSTRSYVTTSTRTYSLGSALRPTTSRTLYTSSPGGVYATRSSAVRLRSGVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAESTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDMDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQTLTCEVDALKGTNESLERQMREMEENFSVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE becn1_bovin Beclin-1 BECN1 protein protein bovin Bos taurus (Bovine) Bovine MEGSKTSSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLATAQLKPGETQEEEANSGEEPFIETRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLGLELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQMQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK vime_human Vimentin VIM protein protein human Homo sapiens Human MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE q8uzc1_pro-vim-2 1424535 n/a 1424539 unspecified role unspecified role bait bait undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false 2C protein sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1424540 unspecified role unspecified role prey prey physical association physical association false false false 1A and 5B q8uzc1_pro-becn1-3 1424535 n/a 1424541 unspecified role unspecified role prey prey n/a 1424539 unspecified role unspecified role bait bait 2C protein sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5B q8uzc1_pro-vim-1 1424536 n/a 1424540 unspecified role unspecified role prey prey n/a 1424539 unspecified role unspecified role bait bait 2C protein sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure vim-q8uzc1_pro-1 1424537 n/a 1424542 unspecified role unspecified role bait bait n/a 1424539 unspecified role unspecified role prey prey infection infection 2C protein sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B q8uzc1_pro-vim-4 1424538 n/a 1424542 unspecified role unspecified role neutral component neutral component n/a 1424539 unspecified role unspecified role neutral component neutral component infection infection 2C protein sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 1C and 3C Transitory colocalization between 0.5 and 1.5 hpi, with vimentin forming a cage-like structure around 2C in all cells infected with FMDV. In presence of a dominant-negative vimentin construct (transfected HA-tagged deletion construct 1-134), the vimentin cage was also formed after 0.5 hpi but remains unaltered at least until 4 hpi.