I2D I2D li-2012p-6 Rab26 modulates the cell surface transport of α2-adrenergic receptors from the Golgi. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li C., Fan Y., Lan T.H., Lambert N.A., Wu G. guwu@georgiahealth.edu J. Biol. Chem. (0021-9258) 2012 imex curation Only protein-protein interactions li-2012p-4 Rab26 modulates the cell surface transport of α2-adrenergic receptors from the Golgi. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Li C., Fan Y., Lan T.H., Lambert N.A., Wu G. guwu@georgiahealth.edu J. Biol. Chem. (0021-9258) 2012 imex curation Only protein-protein interactions li-2012p-5 Rab26 modulates the cell surface transport of α2-adrenergic receptors from the Golgi. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Li C., Fan Y., Lan T.H., Lambert N.A., Wu G. guwu@georgiahealth.edu J. Biol. Chem. (0021-9258) 2012 imex curation Only protein-protein interactions rab26_human Ras-related protein Rab-26 RAB26 protein protein human Homo sapiens Human MSRKKTPKSKGASTPAASTLPTANGARPARSGTALSGPDAPPNGPLQPGRPSLGGGVDFYDVAFKVMLVGDSGVGKTCLLVRFKDGAFLAGTFISTVGIDFRNKVLDVDGVKVKLQMWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKASFDNIQAWLTEIHEYAQHDVALMLLGNKVDSAHERVVKREDGEKLAKEYGLPFMETSAKTGLNVDLAFTAIAKELKQRSMKAPSEPRFRLHDYVKREGRGASCCRP ada2b_human Alpha-2B adrenergic receptor ADRA2B ADRA2L1 ADRA2RL1 Alpha-2 adrenergic receptor subtype C2 Alpha-2B adrenoreceptor protein protein human Homo sapiens Human MDHQDPYSVQATAAIAAAITFLILFTIFGNALVILAVLTSRSLRAPQNLFLVSLAAADILVATLIIPFSLANELLGYWYFRRTWCEVYLALDVLFCTSSIVHLCAISLDRYWAVSRALEYNSKRTPRRIKCIILTVWLIAAVISLPPLIYKGDQGPQPRGRPQCKLNQEAWYILASSIGSFFAPCLIMILVYLRIYLIAKRSNRRGPRAKGGPGQGESKQPRPDHGGALASAKLPALASVASAREVNGHSKSTGEKEEGETPEDTGTRALPPSWAALPNSGQGQKEGVCGASPEDEAEEEEEEEEEEEECEPQAVPVSPASACSPPLQQPQGSRVLATLRGQVLLGRGVGAIGGQWWRRRAQLTREKRFTFVLAVVIGVFVLCWFPFFFSYSLGAICPKHCKVPHGLFQFFFWIGYCNSSLNPVIYTIFNQDFRRAFRRILCRPWTQTAW adra2b-rab26-10 1472868 n/a 1472871 unspecified role unspecified role prey prey rab26-gfp gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9ULW5:p.Thr77Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength anti tag western anti tag western blot anti tag western certain certain sequence position certain certain certain sequence position certain false Q9ULW5:p.Asn177Ile mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength anti tag western anti tag western blot anti tag western certain certain sequence position certain certain certain sequence position certain false rab26gln-123-leu-gfp gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1472872 unspecified role unspecified role bait bait ada2b-icl3 polyprotein frag polyprotein fragment polyprotein frag anti tag western anti tag western blot anti tag western certain certain sequence position certain certain certain sequence position certain false ada2b-icl3-gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1472880 1472881 physical association physical association false false false Fig7 adra2b-rab26-12 1472868 n/a 1472871 unspecified role unspecified role prey prey in vitro In vitro n/a ICL3 1472872 unspecified role unspecified role bait bait icl3-gst polyprotein frag polyprotein fragment polyprotein frag certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false fig 7c The interaction of Rab26 with the GST-ICL3 was more potent in the present of GTP-gamma-S than in the presence of GDP adra2b-rab26-2 1472869 n/a 1472872 unspecified role unspecified role bait bait ada2b-ha ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1472871 unspecified role unspecified role prey prey Q9ULW5:p.Thr77Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9ULW5:p.Asn177Ile mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false rab26gln123leu gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 6a adra2b-rab26-11 1472870 n/a 1472872 unspecified role unspecified role luminescence accept luminescence acceptor luminescence accept region venus venus fluorescent protein tag venus feature detection feature detection method feature detection undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1472871 unspecified role unspecified role luminescence donor luminescence donor Q9ULW5:p.Thr77Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9ULW5:p.Asn177Ile mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region renilla luciferase renilla luciferase protein tag renilla luciferase undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig6B