UniProt UniProt http://www.uniprot.org petit-2012-1 The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Petit A.P., Wohlbold L., Bawankar P., Huntzinger E., Schmidt S., Izaurralde E., Weichenrieder O. Identification for CCR4A/B was found in Reference 13, Lau et al., 2009. oliver.weichenrieder@tuebingen.mpg.de Nucleic Acids Res. (0305-1048) 2012 mimix curation petit-2012-4 The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Petit A.P., Wohlbold L., Bawankar P., Huntzinger E., Schmidt S., Izaurralde E., Weichenrieder O. Identification for CCR4A/B was found in Reference 13, Lau et al., 2009. oliver.weichenrieder@tuebingen.mpg.de Nucleic Acids Res. (0305-1048) 2012 mimix curation petit-2012-2 The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Petit A.P., Wohlbold L., Bawankar P., Huntzinger E., Schmidt S., Izaurralde E., Weichenrieder O. Identification for CCR4A/B was found in Reference 13, Lau et al., 2009. oliver.weichenrieder@tuebingen.mpg.de Nucleic Acids Res. (0305-1048) 2012 mimix curation petit-2012-3 The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Petit A.P., Wohlbold L., Bawankar P., Huntzinger E., Schmidt S., Izaurralde E., Weichenrieder O. Identification for CCR4A/B was found in Reference 13, Lau et al., 2009. oliver.weichenrieder@tuebingen.mpg.de Nucleic Acids Res. (0305-1048) 2012 mimix curation petit-2012-5 The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Petit A.P., Wohlbold L., Bawankar P., Huntzinger E., Schmidt S., Izaurralde E., Weichenrieder O. Identification for CCR4A/B was found in Reference 13, Lau et al., 2009. oliver.weichenrieder@tuebingen.mpg.de Nucleic Acids Res. (0305-1048) 2012 mimix curation cnot7_human CCR4-NOT transcription complex subunit 7 CNOT7 CAF1 CCR4-associated factor 1 BTG1-binding factor 1 Caf1a protein protein human Homo sapiens Human MPAATVDHSQRICEVWACNLDEEMKKIRQVIRKYNYVAMDTEFPGVVARPIGEFRSNADYQYQLLRCNVDLLKIIQLGLTFMNEQGEYPPGTSTWQFNFKFNLTEDMYAQDSIELLTTSGIQFKKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGYDFGYLIKILTNSNLPEEELDFFEILRLFFPVIYDVKYLMKSCKNLKGGLQEVAEQLELERIGPQHQAGSDSLLTGMAFFKMREMFFEDHIDDAKYCGHLYGLGSGSSYVQNGTGNAYEEEANKQS cnot1_human CCR4-NOT transcription complex subunit 1 CNOT1 CDC39 KIAA1007 NOT1 Negative regulator of transcription subunit 1 homolog CCR4-associated factor 1 AD-005 protein protein human Homo sapiens Human MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLINPEIFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPPETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMTSLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVSTMVTTSTTTTVAKTVTVTRPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSVVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPPITTTTTSTTPATNTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS cnot8_human CCR4-NOT transcription complex subunit 8 CNOT8 CALIF POP2 CCR4-associated factor 8 CAF1-like protein CAF2 Caf1b protein protein human Homo sapiens Human MPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVDLLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELLMTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQEVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEKMSILAIINNMQQ cnot6_human CCR4-NOT transcription complex subunit 6 CNOT6 CCR4 KIAA1194 Carbon catabolite repressor protein 4 homolog CCR4 carbon catabolite repression 4-like Cytoplasmic deadenylase CCR4a protein protein human Homo sapiens Human MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSASLWSLTHLTALHLSDNSLSRIPSDIAKLHNLVYLDLSSNKIRSLPAELGNMVSLRELHLNNNLLRVLPFELGKLFQLQTLGLKGNPLTQDILNLYQEPDGTRRLLNYLLDNLSGTAKRITTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLYGYCPSWALNWDYRKKAIIQEILSCNADIVSLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKESIEMPSGKPHLGTEKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRNLKSSVLGEFGTIPLVLCADLNSLPDSGVVEYLSTGGVETNHKDFKELRYNESLTNFSCHGKNGTTNGRITHGFKLQSAYESGLMPYTNYTFDFKGIIDYIFYSKPQLNTLGILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIHLPGRR cno6l_human CCR4-NOT transcription complex subunit 6-like CCR4B Carbon catabolite repressor protein 4 homolog B CNOT6L protein protein human Homo sapiens Human MRLIGMPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNYLSRIPPDIAKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNNNLLRVLPYELGRLFQLQTLGLKGNPLSQDILNLYQDPDGTRKLLNFMLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKGIMEEIVNCDADIISLQEVETEQYFTLFLPALKERGYDGFFSPKSRAKIMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGAGMKPIHAADKQLLIVANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVVEYLSNGGVADNHKDFKELRYNECLMNFSCNGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDYIFYSKTHMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR a8dy81_drome Not1 CG1874 anon-WO0118547.221 clone 1.29 not1 CG34407 Dmel_CG34407 CG1884 Dmel\CG34407 NOT NOT1 anon-EST:Liang-1.29 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNVESQLKTPPTHIRNLVKHVNKRNFNESSEQINQFVKEHGLEADRSSLRHLFSVINFSDLVPSVTVQLQAKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKSLKPVAVADLVGQVARLTGINKVCECVFALALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCLSEYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEADALNEVGIEDIYDHLSEIIFTNQGKNNIMDTSWINLILEIGYEFTSSVEECKNHLCSRERERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFNQLIPTFLGNHPNSNVILASAWSSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPEDQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVPPPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGPGMDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAAAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKALLSGLNGMPSMAEIQAAAMQLASENVELVCAFIQKTSAEKAAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSIPGFQQMSDRDIALFVPKPTDLSQPNVFANDDSMVYGELASKMEAFMNTAIGVPTLQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNNSFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSSSDYSPFNGNDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQAKNKIFQWIDAFVHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEGQEVATIN e1jhy7_drome Poly(A)-specific ribonuclease Pop2 CAF1 Caf1 Dmel\CG5684 POP2 pop2 Dmel_CG5684 CNOT7 CG5684 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPSAISGAPHAHIPSNEECGIRDVWKQNLEEEFRTIRKVVQKYHYVAMDTEFPGVVARPVGEFRSTADYHYQLLRCNVDLLRIIQLGLTFMDDDGKTPPGYSTWQFNFKFNLSEDMYAQDSIDLLQNSGIQFKKHEEDGIDPIDFAELLMSSGIVLVENIKWLCFHSGYDFGYLLKLLTDQNLPPDESEFFDLLHIYFPNIFDIKYLMKSCKNLKGGLQEVADQLELRRVGPQHQAGSDALLTGMAFFKMREVQHTNDFHITPVAHDVLFRYLLEHTMNLKPTISCIHLLSRPAYNGGPAPDESAIISSGNGTGNTNSTEQFTSSAYMVTPPNTPGMAKSDAASLSNRHTSISNSFG q7k112_drome Poly(A)-specific ribonuclease twin BEST:GH20274 CT39331 Ccr4 Dmel\CG31137 Twin ccr4 twn Dmel_CG31137 CCR4 CG31137 CG17741 CG5534 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKGNHYKMSRNKDKYDSANRRQQIFLSQEDIAAGKKTNWSGLEITGCVRNISPSLWEFEHLTALYLNDNQLLRLPADVGMLTSLRTLDLSSNKLRSLPAELGELIQLRELLLNNNFLRVLPYEIGKLFHLVILGLMGNPLQKEFMNIYNEPNGTQKLLTYMLDNLSFTVNPPPQRPWLPLAKPNKTRPACIFTVMCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQEIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESLIEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHIHWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVVEFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFKGIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQAPPNGLINRR cnot1-cnot7-2 1372529 n/a 1372534 unspecified role unspecified role prey prey n/a 1372535 unspecified role unspecified role bait bait physical association physical association false false false 1B and 7A cnot1-cnot8-1 1372529 n/a 1372535 unspecified role unspecified role bait bait n/a 1372536 unspecified role unspecified role prey prey physical association physical association false false false 1C cnot1-cnot6 1372529 n/a 1372537 unspecified role unspecified role prey prey n/a 1372535 unspecified role unspecified role bait bait physical association physical association false false false 1D cnot1-cnot6l-1 1372529 n/a 1372535 unspecified role unspecified role bait bait n/a 1372538 unspecified role unspecified role prey prey physical association physical association false false false 1E cnot7-cnot1-1 1372529 n/a 1372534 unspecified role unspecified role bait bait n/a 1372535 unspecified role unspecified role prey prey physical association physical association false false false 7B pop2-not1-6 1372530 n/a 1372539 unspecified role unspecified role prey prey n/a 1372540 unspecified role unspecified role bait bait physical association physical association false false false 8A not1-pop2 1372530 n/a 1372540 unspecified role unspecified role prey prey n/a 1372539 unspecified role unspecified role bait bait physical association physical association false false false 8B twin-twin-1 1372530 n/a 1372540 unspecified role unspecified role prey prey n/a 1372541 unspecified role unspecified role bait bait physical association physical association false false false 8C cnot7-cnot1 1372531 n/a 1372534 unspecified role unspecified role bait bait n/a 1372535 unspecified role unspecified role prey prey physical association physical association false false false 2A and B cnot8-cnot1-1 1372531 n/a 1372536 unspecified role unspecified role bait bait n/a 1372535 unspecified role unspecified role prey prey physical association physical association false false false 2A cnot1-cnot7-3 1372532 n/a 1372534 unspecified role unspecified role neutral component neutral component n/a 1372535 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false 3, Table S1. 2.7A working 21.5%, free 24.9 % cnot1-cnot1 1372532 n/a 1372535 unspecified role unspecified role neutral component neutral component Stoichiometry: 6.0 direct interaction direct interaction false false false 3 2.90A working 19.2%, free 22.9 % cnot1-cnot7-4 1372533 n/a 1372534 unspecified role unspecified role neutral component neutral component n/a 1372535 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false S1