UniProt UniProt http://www.uniprot.org perisic-2012-1 Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Perisic L., Lal M., Hulkko J., Hultenby K., Önfelt B., Sun Y., Dunér F., Patrakka J., Betsholtz C., Uhlen M., Brismar H., Tryggvason K., Wernerson A., Pikkarainen T. Colocalization exp related to F-actin not included as the actin protein cannot be mapped to UniProt. karl.tryggvason@ki.se Kidney Int. (0085-2538) 2012 mimix curation perisic-2012-3 Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Perisic L., Lal M., Hulkko J., Hultenby K., Önfelt B., Sun Y., Dunér F., Patrakka J., Betsholtz C., Uhlen M., Brismar H., Tryggvason K., Wernerson A., Pikkarainen T. Colocalization exp related to F-actin not included as the actin protein cannot be mapped to UniProt. karl.tryggvason@ki.se Mouse kidney glomerularcomplementary DNA Kidney Int. (0085-2538) 2012 mimix curation perisic-2012-4 Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Perisic L., Lal M., Hulkko J., Hultenby K., Önfelt B., Sun Y., Dunér F., Patrakka J., Betsholtz C., Uhlen M., Brismar H., Tryggvason K., Wernerson A., Pikkarainen T. Colocalization exp related to F-actin not included as the actin protein cannot be mapped to UniProt. karl.tryggvason@ki.se Kidney Int. (0085-2538) 2012 mimix curation perisic-2012-2 Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics. human-podocyte human podocyte podocyte renal glomerular podocyte fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Perisic L., Lal M., Hulkko J., Hultenby K., Önfelt B., Sun Y., Dunér F., Patrakka J., Betsholtz C., Uhlen M., Brismar H., Tryggvason K., Wernerson A., Pikkarainen T. Colocalization exp related to F-actin not included as the actin protein cannot be mapped to UniProt. karl.tryggvason@ki.se Kidney Int. (0085-2538) 2012 mimix curation perisic-2012-5 Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics. human Homo sapiens Human electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy immunostaining immunostaining Perisic L., Lal M., Hulkko J., Hultenby K., Önfelt B., Sun Y., Dunér F., Patrakka J., Betsholtz C., Uhlen M., Brismar H., Tryggvason K., Wernerson A., Pikkarainen T. Colocalization exp related to F-actin not included as the actin protein cannot be mapped to UniProt. karl.tryggvason@ki.se Kidney Int. (0085-2538) 2012 mimix curation perisic-2012-6 Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics. human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Perisic L., Lal M., Hulkko J., Hultenby K., Önfelt B., Sun Y., Dunér F., Patrakka J., Betsholtz C., Uhlen M., Brismar H., Tryggvason K., Wernerson A., Pikkarainen T. Colocalization exp related to F-actin not included as the actin protein cannot be mapped to UniProt. karl.tryggvason@ki.se Kidney Int. (0085-2538) 2012 mimix curation pkhh2_mouse Pleckstrin homology domain-containing family H member 2 Plekhh2 Kiaa2028 protein protein mouse Mus musculus Mouse MEEPSEPEGLIDWKERCVALEAQLMKFRVQASKIRELLADKMQQLERQVIDAERQAEKAFQEVQVMEEKLKAANIQTSESETRLYKKCQDLESVMQEKDDIIQNLALRLEEQKQVRIQEAKIIEEKAAKIKEWVTVKLNELEVENQNLRFINQTQTEEIRAIQSKLQELQEKKISCVSSPKTSEGQRNLTFGCFLSRAKSPPCVVRCEEVSKMASNEPEITEGRCVEEMEIAEKPADNQVQENSRSQRKLHETSCSSEQNQKTRASFAMDGGTSQNSGVPVSDWSSDEDDGSKGRSKSRCTSTLSSHTSEEGGQCGRLGSEAYLTASDDSSSIFEEETFDGNRPEQKKLCSWQQKAPWKAQGNLAKGRSQSGVKEQDSSSDELNKKFHSQRLDYTSSSSEANTPSPILTPALTPRYPNSLPGKGGAPLVPPPFQPPPKLRVPNVFSISVALTKRHLSQPQLCSDRMFGTNRNAISMIRPLRPQETDLDVVDGDGAEAVNRMDTGCDDGLFSYDSQDPPPCADDQENSEAPKKAPCNKPPTPPLHRFPSWESRIYAVAKSGIRVSEAFNMEHANKNSADILSYSAASLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSDEEDSSRSSSRLSESDARSRSGPSSPRAMKRGVSDSSAASESDYAIPPDAYPIDAECSQPEQKLLKTCLASCDNGKNEPLEKSGYLLKMSVRVKTWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLATEKHTYYLTADSPNILEEWIKVLQSVLRVQAANPLCLQPEGKPAVKGLLTKVKHGYSKRVWCMLVGKVLYYFRNQEDKFPLGQLKLWEAKVEEVDRSCDSDEDYETRGCYLLSTHYTIIVHPKDQGPTYLLIGSKHEKEAWLYHLTVAAGSNNINVGSEFEQLVCKLLNIEGEPSSQIWRHPMLCHSKEGILSPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQVCLTHPELQNEICCQLIKQTRRRQLQNQPGPLQGWQLLALCVGLFLPHHPFLWLLQLHLQRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSRPFSIPVHFMNGLYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKCEGSRTVRLTYKNRLYFSVQARGETDREKTLLLYQTNDQIINGLFPLNKDLALEMAALLAQVDIGDFERPFSTPAGPVTNQCKANQTLKQVIERFYPKRYREGCSEEQLRQLYQRLSTKWMALRGHSAADCIRIYLTVARKWPFFGAKLFFAKPIAPSSLGNNCVWLAVHENGLSILEYTSMRLVTSYMYKGLMTFGGYQEDFMVVVSTQSKDRPTEKLLFAMAKHKILEITLLIASYINSFHQQKTTFHHLSAPALLSPRTQAPQARVMGSQPPLSNSRPTKGPTLL tgfi1_mouse Transforming growth factor beta-1-induced transcript 1 protein Tgfb1i1 Ara55 Hydrogen peroxide-inducible clone 5 protein Androgen receptor-associated protein of 55 kDa TGF beta-stimulated clone 5 protein protein mouse Mus musculus Mouse MEDLDALLSDLETTTSHMSRLGAPKERPPETLTPPPPYGHQPQTGSGESSGTTGDKDHLYSTVCKPRSPKPVAPVAPPFSSSSGVLGNGLCELDRLLQELNATQFNITDEIMSQFPSSKMAEGEEKEDQSEDKSSPTVPPSPFPAPSKPSATSATQELDRLMASLSDFRVQNHLPASGPPQPPAASPTREGCPSPPGQTSKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFLCSGCSTTLGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGEEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECLAPFSGGSFFEHEGRPLCENHFHAQRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERASKPYCQPCFLKLFG actb_mouse Actin, cytoplasmic 1 Actb Beta-actin protein protein mouse Mus musculus Mouse MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF pkhh2_human Pleckstrin homology domain-containing family H member 2 PLEKHH2 KIAA2028 protein protein human Homo sapiens Human MAELSEPEGPVDWKERCVALESQLMKFRVQASKIRELLAEKMQQLERQVIDAERQAEKAFQQVQVMEDKLKAANIQTSESETRLYNKCQDLESLIQEKDDVIQNLELQLEEQKQIRIQEAKIIEEKAAKIKEWVTVKLNELELENQNLRLINQNQTEEIRTMQSKLQEVQGKKSSTVSTLKLSEGQRLSSLTFGCFLSRARSPPQVVKSEEMSKISSKEPEFTEGKDMEEMEIPEKSVDNQVLENNRGQRTLHQTPCGSEQNRKTRTSFATDGGISQNSGAPVSDWSSDEEDGSKGRSKSRCTSTLSSHTSEEGVQCSRMGSEMYLTASDDSSSIFEEETFGIKRPEHKKLYSWQQEAQWKALNSPLGKGNSELSKKEQDSSSDELNKKFQSQRLDYSSSSSEANTPSPILTPALMPKHPNSLSGKGTQLVPSSHLPPPKLRIPNVFSISVALAKRHLSQPQLSSDRMFGTNRNAISMIRPLRPQETDLDLVDGDSTEVLENMDTSCDDGLFSYDSLDSPNSDDQEHCDSAKKVAYSKPPTPPLHRFPSWESRIYAVAKSGIRMSEAFNMESVNKNSAATLSYTTSGLYTSLIYKNMTTPVYTTLKGKATQISSSPFLDDSSGSEEEDSSRSSSRTSESDSRSRSGPGSPRAMKRGVSLSSVASESDYAIPPDAYSTDTEYSQPEQKLPKTCSSSSDNGKNEPLEKSGYLLKMSGKVKSWKRRWFVLKGGELLYYKSPSDVIRKPQGHIELSASCSILRGDNKQTVQLTTEKHTYYLTADSPNILEEWIKVLQNVLRVQAANPLSLQPEGKPTMKGLLTKVKHGYSKRVWCTLIGKTLYYFRSQEDKFPLGQIKLWEAKVEEVDRSCDSDEDYEASGRSLLSTHYTIVIHPKDQGPTYLLIGSKHEKDTWLYHLTVAAGSNNVNVGSEFEQLVCKLLNIDGEPSSQIWRHPTLCHSKEGIISPLTTLPSEALQTEAIKLFKTCQLFINAAVDSPAIDYHISLAQSALQICLTHPELQNEICCQLIKQTRRRQPQNQPGPLQGWQLLALCVGLFLPHHPFLWLLRLHLKRNADSRTEFGKYAIYCQRCVERTQQNGDREARPSRMEILSTLLRNPYHHSLPFSIPVHFMNGIYQVVGFDASTTVEEFLNTLNQDTGMRKPAQSGFALFTDDPSGRDLEHCLQGNIKICDIISKWEQASKEQQPGKCEGTRTVRLTYKNRLYFSVQARGETDREKLLLMYQTNDQIINGLFPLNKDLALEMAALLSQVEIGDFERPFSTPAGHVTNQCKVNQTLKQVIEKFYPKRYRDGCSEEQLRQLCQRLSTRWMALRGHSAADCVRIYLTVARKWPFFGAKLFLAKPITPSSLGSTFLWLAVHEDGLSLLEYNSMRLIVSYVYKSLMTFGGYQDDFMVVINNTHSKDKPTEKLLFAMAKPKILEITLLIASYINNFHQQKAAFHHLSAPALLSAQTRGPQARMMGSQPLLSSSRPTKGPTLL tgfi1_human Transforming growth factor beta-1-induced transcript 1 protein TGFB1I1 ARA55 Hydrogen peroxide-inducible clone 5 protein Androgen receptor-associated protein of 55 kDa Androgen receptor coactivator 55 kDa protein protein protein human Homo sapiens Human MEDLDALLSDLETTTSHMPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGDEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECFAPFSGGSFFEHEGRPLCENHFHARRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERAGKPYCQPCFLKLFG plekhh2-plekhh2-4 1364527 n/a 1364533 unspecified role unspecified role bait bait n/a 1364533 unspecified role unspecified role prey prey physical association physical association false false false 5a plekhh2-tgfb1i1-2 1364527 n/a 1364534 unspecified role unspecified role prey prey n/a 1364533 unspecified role unspecified role bait bait physical association physical association false false false 6b and S7 plekhh2-tgfb1i1 1364528 n/a 1364533 unspecified role unspecified role bait bait n/a 1364534 unspecified role unspecified role prey prey physical association physical association false false false no figure plekhh2-actb-3 1364528 n/a 1364533 unspecified role unspecified role bait bait n/a 1364535 unspecified role unspecified role prey prey physical association physical association false false false no figure plekhh2-tgfb1i1-3 1364529 n/a 1364533 unspecified role unspecified role bait bait n/a 1364534 unspecified role unspecified role prey prey physical association physical association false false false 6a and S8 plekhh2-actb-2 1364529 n/a 1364535 unspecified role unspecified role prey prey n/a 1364533 unspecified role unspecified role bait bait physical association physical association false false false 6a and S8 plekhh2-plekhh2-3 1364530 n/a 1364533 unspecified role unspecified role neutral component neutral component n/a 1364533 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 5c plekhh2-tgfb1i1-4 1364531 n/a 1364536 unspecified role unspecified role neutral component neutral component n/a 1364537 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 7 plekhh2-tgfb1i1-5 1364532 n/a 1364536 unspecified role unspecified role bait bait n/a 1364534 unspecified role unspecified role prey prey physical association physical association false false false S7a left