UniProt UniProt http://www.uniprot.org grill-2012-3 RAE-1, a novel PHR binding protein, is required for axon termination and synapse formation in Caenorhabditis elegans. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Grill B., Chen L., Tulgren E.D., Baker S.T., Bienvenut W., Anderson M., Quadroni M., Jin Y., Garner C.C. bgrill@umn.edu J. Neurosci. (0270-6474) 2012 Only protein-protein interactions imex curation grill-2012-1 RAE-1, a novel PHR binding protein, is required for axon termination and synapse formation in Caenorhabditis elegans. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Grill B., Chen L., Tulgren E.D., Baker S.T., Bienvenut W., Anderson M., Quadroni M., Jin Y., Garner C.C. bgrill@umn.edu J. Neurosci. (0270-6474) 2012 Only protein-protein interactions imex curation grill-2012-2 RAE-1, a novel PHR binding protein, is required for axon termination and synapse formation in Caenorhabditis elegans. caeel Caenorhabditis elegans confocal microscopy confocal microscopy immunostaining immunostaining Grill B., Chen L., Tulgren E.D., Baker S.T., Bienvenut W., Anderson M., Quadroni M., Jin Y., Garner C.C. bgrill@umn.edu J. Neurosci. (0270-6474) 2012 Only protein-protein interactions imex curation rae1l_rat mRNA export factor Rae1 Mrnp41 Rae1 protein homolog mRNA-associated protein mrnp 41 protein protein rat Rattus norvegicus (Rat) Rat MSLFGTTSGFGTGGTSMFGSTTTDNHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWANDVRCWEVQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLNSNQAIQIAQHDAPVKTIHWIKAPNYSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLIVYQLENQPSEFRRIESPLKHQHRCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPVAACGFNHNGNIFAYASSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK mycb2_human E3 ubiquitin-protein ligase MYCBP2 MYCBP2 KIAA0916 PAM Myc-binding protein 2 Protein associated with Myc protein protein human Homo sapiens Human MMMCAATASPAAASSGLGGDGFYPAATFSSSPAPGALFMPVPDGSVAAAGLGLGLPAADSRGHYQLLLSGRALADRYRRIYTAALNDRDQGGGSAGHPASRNKKILNKKKLKRKQKSKSKVKTRSKSENLENTVIIPDIKLHSNPSAFNIYCNVRHCVLEWQKKEISLAAASKNSVQSGESDSDEEEESKEPPIKLPKIIEVGLCEVFELIKETRFSHPSLCLRSLQALLNVLQGQQPEGLQSEPPEVLESLFQLLLEITVRSTGMNDSTGQSLTALSCACLFSLVASWGETGRTLQAISAILTNNGSHACQTIQVPTILNSLQRSVQAVLVGKIQIQDWFSNGIKKAALMHKWPLKEISVDEDDQCLLQNDGFFLYLLCKDGLYKIGSGYSGTVRGHIYNSTSRIRNRKEKKSWLGYAQGYLLYRDVNNHSMTAIRISPETLEQDGTVMLPDCHTEGQNILFTDGEYINQIAASRDDGFVVRIFATSTEPVLQQELQLKLARKCLHACGISLFDLEKDLHIISTGFDEESAILGAGREFALMKTANGKIYYTGKYQSLGIKQGGPSAGKWVELPITKSPKIVHFSVGHDGSHALLVAEDGSIFFTGSASKGEDGESTKSRRQSKPYKPKKIIKMEGKIVVYTACNNGSSSVISKDGELYMFGKDAIYSDSSSLVTDLKGHFVTQVAMGKAHTCVLMKNGEVWTFGVNNKGQCGRDTGAMNQGGKGFGVENMATAMDEDLEEELDEKDEKSMMCPPGMHKWKLEQCMVCTVCGDCTGYGASCVSSGRPDRVPGGICGCGSGESGCAVCGCCKACARELDGQEARQRGILDAVKEMIPLDLLLAVPVPGVNIEEHLQLRQEEKRQRVIRRHRLEEGRGPLVFAGPIFMNHREQALARLRSHPAQLKHKRDKHKDGSGERGEKDASKITTYPPGSVRFDCELRAVQVSCGFHHSVVLMENGDVYTFGYGQHGQLGHGDVNSRGCPTLVQALPGPSTQVTAGSNHTAVLLMDGQVFTFGSFSKGQLGRPILDVPYWNAKPAPMPNIGSKYGRKATWIGASGDQTFLRIDEALINSHVLATSEIFASKHIIGLVPASISEPPPFKCLLINKVDGSCKTFNDSEQEDLQGFGVCLDPVYDVIWRFRPNTRELWCYNAVVADARLPSAADMQSRCSILSPELALPTGSRALTTRSHAALHILGCLDTLAAMQDLKMGVASTEEETQAVMKVYSKEDYSVVNRFESHGGGWGYSAHSVEAIRFSADTDILLGGLGLFGGRGEYTAKIKLFELGPDGGDHETDGDLLAETDVLAYDCAAREKYAMMFDEPVLLQAGWWYVAWARVSGPSSDCGSHGQASITTDDGVVFQFKSSKKSNNGTDVNAGQIPQLLYRLPTSDGSASKGKQQTSEPVHILKRSFARTVSVECFESLLSILHWSWTTLVLGVEELRGLKGFQFTATLLDLERLRFVGTCCLRLLRVYTCEIYPVSATGKAVVEETSKLAECIGKTRTLLRKILSEGVDHCMVKLDNDPQGYLSQPLSLLEAVLQECHNTFTACFHSFYPTPALQWACLCDLLNCLDQDIQEANFKTSSSRLLAAVMSALCHTSVKLTSIFPIAYDGEVLLRSIVKQVSTENDSTLVHRFPLLVAHMEKLSQSEENISGMTSFREVLEKMLVIVVLPVRNSLRRENELFSSHLVSNTCGLLASIVSELTASALGSEVDGLNSLHSVKASANRFTKTSQGRSWNTGNGSPDAICFSVDKPGIVVVGFSVYGGGGIHEYELEVLVDDSEHAGDSTHSHRWTSLELVKGTYTTDDSPSDIAEIRLDKVVPLKENVKYAVRLRNYGSRTANGDGGMTTVQCPDGVTFTFSTCSLSSNGTNQTRGQIPQILYYRSEFDGDLQSQLLSKANEEDKNCSRALSVVSTVVRASKDLLHRALAVDADDIPELLSSSSLFSMLLPLIIAYIGPVAAAIPKVAVEVFGLVQQLLPSVAILNQKYAPPAFNPNQSTDSTTGNQPEQGLSACTTSSHYAVIESEHPYKPACVMHYKVTFPECVRWMTIEFDPQCGTAQSEDVLRLLIPVRTVQNSGYGPKLTSVHENLNSWIELKKFSGSSGWPTMVLVLPGNEALFSLETASDYVKDDKASFYGFKCFAIGYEFSPGPDEGVIQLEKELANLGGVCAAALMKKDLALPIGNELEEDLEILEEAALQVCKTHSGILGKGLALSHSPTILEALEGNLPLQIQSNEQSFLDDFIACVPGSSGGRLARWLQPDSYADPQKTSLILNKDDIRCGWPTTITVQTKDQYGDVVHVPNMKVEVKAVPVSQKKMSLQQDQAKKPQRIPGSPAVTAASSNTDMTYGGLASPKLDVSYEPMIVKEARYIAITMMKVYENYSFEELRFASPTPKRPSENMLIRVNNDGTYCANWTPGAIGLYTLHVTIDGIEIDAGLEVKVKDPPKGMIPPGTQLVKPKSEPQPNKVRKFVAKDSAGLRIRSHPSLQSEQIGIVKVNGTITFIDEIHNDDGVWLRLNDETIKKYVPNMNGYTEAWCLSFNQHLGKSLLVPVDESKTNTDDFFKDINSCCPQEATMQEQDMPFLRGGPGMYKVVKTGPSGHNIRSCPNLRGIPIGMLVLGNKVKAVGEVTNSEGTWVQLDQNSMVEFCESDEGEAWSLARDRGGNQYLRHEDEQALLDQNSQTPPPSPFSVQAFNKGASCSAQGFDYGLGNSKGDRGNISTSSKPASTSGKSELSSKHSRSLKPDGRMSRTTADQKKPRGTESLSASESLILKSDAAKLRSDSHSRSLSPNHNTLQTLKSDGRMPSSSRAESPGPGSRLSSPKPKTLPANRSSPSGASSPRSSSPHDKNLPQKSTAPVKTKLDPPRERSKSDSYTLDPDTLRKKKMPLTEPLRGRSTSPKPKSVPKDSTDSPGSENRAPSPHVVQENLHSEVVEVCTSSTLKTNSLTDSTCDDSSEFKSVDEGSNKVHFSIGKAPLKDEQEMRASPKISRKCANRHTRPKKEKSSFLFKGDGSKPLEPAKQAMSPSVAECARAVFASFLWHEGIVHDAMACSSFLKFHPELSKEHAPIRSSLNSQQPTEEKETKLKNRHSLEISSALNMFNIAPHGPDISKMGSINKNKVLSMLKEPPLHEKCEDGKTETTFEMSMHNTMKSKSPLPLTLQHLVAFWEDISLATIKAASQNMIFPSPGSCAVLKKKECEKENKKSKKEKKKKEKAEVRPRGNLFGEMAQLAVGGPEKDTICELCGESHPYPVTYHMRQAHPGCGRYAGGQGYNSIGHFCGGWAGNCGDGGIGGSTWYLVCDRCREKYLREKQAAAREKVKQSRRKPMQVKTPRALPTMEAHQVIKANALFLLSLSSAAEPSILCYHPAKPFQSQLPSVKEGISEDLPVKMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYLRSTSVPAPYISVTPDASPNVFEEPESNMKSMPPSLETSPITDTDLAKRTVFQRSYSVVASEYDKQHSILPARVKAIPRRRVNSGDTEVGSSLLRHPSPELSRLISAHSSLSKGERNFQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVFAMEAFNWLLCNVIQTTSLHDILWHFVASLTPAPVEPEEEEDEENKTSKENSEQEKDTRVCEHPLSDIVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSSLQQMALRCWSLKFKQSDHQFLHQSNVFHHINNILSKSDDGDSEESFSISIQSGFEAMSQELCIVMCLKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKNITINCVKGINARYVSVHVDNSRDLGNKVTSMTFLTGKAVEDLCRIKQVDLDSRHIGWVTSELPGGDNHIIKIELKGPENTLRVRQVKVLGWKDGESTKIAGQISASVAQQRNCEAETLRVFRLITSQVFGKLISGDAEPTPEQEEKALLSSPEGEEKVYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRVREEWEHAISSKENANSQPNDEDASSDAYCFELLSMVLALSGSNVGRQYLAQQLTLLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPSRLASIIGVKSLPPADISDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLKAKGTTITGTAGTTVGKGVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQISEIIIKLIKDMAAGHLSEAWSRVTKNAIAETIIALTKMEEEFRSPVRCIATTRLWLALASLCVLDQDHVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNVCGNLCTDCDRFLHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKTMKAMVEFREHTGKPTTSSSEACRFCGSRSGTELSAVGSVCSDADCQEYAKIACSKTHPCGHPCGGVKNEEHCLPCLHGCDKSATSLKQDADDMCMICFTEALSAAPAIQLDCSHIFHLQCCRRVLENRWLGPRITFGFISCPICKNKINHIVLKDLLDPIKELYEDVRRKALMRLEYEGLHKSEAITTPGVRFYNDPAGYAMNRYAYYVCYKCRKAYFGGEARCDAEAGRGDDYDPRELICGACSDVSRAQMCPKHGTDFLEYKCRYCCSVAVFFCFGTTHFCNACHDDFQRMTSIPKEELPHCPAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVCRNAHTF rpm1_caeel E3 ubiquitin-protein ligase rpm-1 rpm-1 syd-3 Pam/highwire/rpm-1 protein Regulator of presynaptic morphology protein 1 Synapse defective protein 3 C01B7.6 protein protein caeel Caenorhabditis elegans MSFFLREIDGERVCSTSTETSIPEDRKSARRLRKLLYKCAENTCKPDILRVPAHCGKLDKEEEKSKTPLDQQQQINFHDILNSKFVVGPPSPFALVAIAKSILARFGNPEEVSEKEDGSEEEAGTSGADEEGKQYKNTDQLVGASLTCIFEVLEQLSRRDAELCVQALESLLSLIQSMPIDCLQSENRLSMSAMMHVLKTLREDACPSVSSKATSCLVALSVACGEPEHLGSTIRSLICMKKNIRMSADSTYDMIQMPENLRKLLLKVRRKALGGDNTANSPPNWAMVDVHEHSVASSFSLPSLPDSSPSSDTPDDDNRIHSTMACDGTFLYILNYVGLYKLGTGLNETISGKLYAANQSLQSSKNVQMYLCNGSLYLRRNYSSCISVIDTDSLLDIGEVILPPSCVQHALFTDGTYFYSATLIANSTLSTIQLNDSFSPSNEPSSRRSHRLTDVKFTIQGDLQVPHQLPEFLPANLHPQTVDLHFTREMAFIQARSGKVYYAGNGTRFGLFETGNNWMELCLPEPIVQISVGIDTIMFRSGAGHGWIASVDDKKRNGRLRRLVPSNRRKIVHVCASGHVYGYVSENGKIFMGGLHTMRVNVSSQMLNGLDNVMISSLALGKSHGVAVTRNGHLFTWGLNNMNQCGRVESTSTTSSPRHSGRQEYQICPIGEHTWLTDTPSVCAQCGLCSARGVACGRVPRPKGTMCHCGVGESTCLRCGLCRPCGEVTEPAQPGRAQHVQFSSTAAPQRSTLHPSRVILSQGPHDVKVSSVSCGNFHTVLLASDRRVFTFGSNCHGQLGVGDTLSKNTPQQVILPSDTVIVQVAAGSNHTILRANDGSVFTFGAFGKGQLARPAGEKAGWNAIPEKVSGFGPGFNAFAGWIGADGDSSIIHSHTALLSSDNILKAQIVANKTNIFIFPREVGKDYIVIRRKLNVFEHHASDYKCWYTSWATDPKYDMLWYYNSAEMEIKGYDIFKKSEKSVGDAFDSLTFLAGAEFAVQVYDSPAYATSMSLGMQLLSATFSANVINLSEFWKEKHGEREQDHEKTIMDGYSVANRFDGTGGGWGYSANSVEAIQFKVSKEIRLVGVGLYGGRGEYISKLKLYRQIGTEADELYVEQITETDETVYDCGAHETATLLFSQPIVIQPNHWHVVSAKISGPSSDCGANGKRHVECDGVTFQFRKSAVSNNGTDVDVGQIPELYYQIVGGSESRDESDSNKQLSISRDMSNLFSPAALKNVTAEGIGNLLILLEWALQRVQIDEDTNNQVEGSAENQWSQERAGFVAILSMKLISRFVRTVYKEKGCHDEPGIDFANKLVNLHSMLLEFFFSTDMTGYENRPLIKKEEKVVEEGYTLMKCVSEAVKLFISLSHCFMGSRSLMNAHLIAVMNKGNHEALILTSAIIGSLAKIERFAHQLLCSTTTTERFPMLSSLLLKHFNCEKETLASLTSFPNILRFLYDQTFMRNAYENTSSLAEAILVKVSRDLAIPTDDTLMGPVVHQTSSRFRRRSAQPTWDMSDGCADAIAFRVDSEGIKLHGFGIYLPTEPDRRNFVGEIMMLSPDSSEKWTCLLRVTAEMSSEEKEVGIVRFPEYVLLSPGVTYAVKVNMMKNTKTFCGEGGVTQVHLLNGARLFFSGCSMSQNGTTVQRGQLPYLIYSILDQSNSLQIKQETIYDTFTLLLRLMANKIGAAITEGGALPACCQHLMSHINPHVMVYMERFPDKALEMMSTMEQLIPMVSNLNGVERVFHSYDSDDSGCDTPYSGIVTTVVESQHPYKPNTSSSMVLLFEEADYICVRFSPDCQTAQFDDQLTIYLKIDEHSYMPIERCYGSEWPSYPMILPGNCLMFVLDASSAVEGATSEQMFGYHVTVTGYLVGYNDSTMRLEQDLVWLSANACRIMTQLPINPSNIEHLSTAEDDTRHLFEKHGSLLKKGLSLSHSPTLSELCTKGQPPPAQSADLQFLREFLSGHTSTSAGFLAKWLPTGSVVDASKCQLSLSHDDLIVGKAVTLKLLCKDQYDREVDCPKLQVEVFASLGHRNPSSTIHQNLHIGNLPSSLLIHQNPFQPIIVNHTRYMNIAAMPAYANYSVEEIRLGFMIEELVKDRVPLKSSDSSLFSGTWTPTTAGKYRIECKVDGSDISHTYTVEVTERPHRAGKGTITKPSGSRRGAQMTVARTVSIPFSSDFSGIRMRLGTTLASTSVGVIPRGALVEFIEEMDNDDGKWIRLTDETALLYGCNQGVGQVWCLAYHRPLQRELIPLKADTDREKAVKLRRKEIEKESNGSKHHSVSIDAKETYILSPNDVLQVYSTPAPHSMIDGEKIIGPCDLMSSGWLANRHGVWIKLTGVEKYVLQKNDPSSETSLSFSTNGNDEEDLERPIERKKTRLPNALTPSVADCIRAVFAAFVWHEHLVKDLMAAAAYLRFHQNLHNIWQSCEIPSCTNAPAALQPIVKIWREICEVVETSVEQHLIMPPVSNKAMRAETVKPPSRSGGCELCDANITVPLTVHLRMAHPGCGGDCLGYGYNSNGKFTTGWSGECGAGGRGQSPWYLLCNTCRSQYLKKTPAGHHQERTRRWREFRFSTSASDARPEVIIRQNAMFLLDLNSRLQTESNSSSTATSGWTINLFPTHLSTPSTMPRSQQKRLDVPPNNSVHQNSYMKLGYSSDPGPKVNVIMSPPNQGADQSASLNRPGPSAINEPAEVLQSPSAALRTLFSNTNPSTSALLKRPVLAFCVEHHDLKRIKSACVQSVRRAVAFSHAFRVWNWLLRMVSSEYSVSDIILQYLTTLTSYNRLAEYMFSAKKNSNILPHPWRLCFLAGPIAADMVTQLHAFLHTVSIILQSAGVDGRLKSLCFKSWTLQLTAQEQDLLILTCNILGTVGGILSDTSILDSDNRFVKEMKDITKFADITASSRQAMVICLTDESGETFWESGEEDKNRSRSLSVQLDESAHGEILSLFIDNARDEGYRISSIAFKAILEDGRRKDLTSLTLESAYCGWLKCCIKDISHIQIQFKGPNPASRIRQLMILGYPAKTTGTPRLAPSTSHHLFFSDTQRDAFALFQAISSQAFCGELSEDDTLRERVIDLLFSRVQLYPLQNYVYTQVVQAMEKEVELLCDKSKRNYSYCCGLMSLLVRICDSRGNMDSFGQRNSVLTSITQLLIFSPVVVQRQCLNSLECIFASFTPSNVEVPKIIRNLLVVVGKVIQLQVRDKAAHTVVTVHLCSSVLNAPQNWRVDKSIDMDIGRQTAVLVENLCNGTYTPEWSNATKCELANCLLSLIQMPESVSYTETLSTGGKSKAVAVVSSKRFWTAISSLSLIKDKSWLELSERWKTVQDEEDQEPVLLCENHDDGHTVAQVFCVDCDVALCKECFTVMHLHKKNRNHGVKNLVQSSTQHDINIHQGCARMKFLNFLILFHGEALNGMVEVAADTLFPTSTSSIQPAMQSSSAFLGIHPMTCRFCGNNVPVEDQSLDGTCTHEDCVNYAKTACQVMHTCNHFCGGIRNEEECLPCMTCKREDAAQDGDDVCVICFTERLGAAPCIRLGCGHMFHFHCVRMILERRWNGPRIVFRFMQCPLCIQPIEHSGLQDLIEPLKTIRQEVVDKAKMRLEYDGLLTTPALTDPRSEFYNQPEEYALDRYMYVLCHKCKKAYFGGESRCQAALDSSQFNPEELLCGGCSDTSGVQVCPRHGVEYLEYKCRFCCSIAVYFCFGTTHFCAPCHDDFQRLMSLPKHLLPTCPVGPRSTPMEEQTCPLKMKHPPTGDEFAMGCGICRNISTF rae1_caeel mRNA export factor rae-1 Nuclear pore complex protein 17 Nucleoporin-17 rae-1 npp-17 F10G8.3 protein protein caeel Caenorhabditis elegans MFGSSGFGNKSMFGGSNISTSTTTPAANTTQNDDFLVDGAPEDTIQVIKFSPTPQDKPMLACGSWDGTIRVWMFNDANTFEGKAQQNIPAPILDIAWIEDSSKIFIACADKEARLWDLASNQVAVVGTHDGPVKTCHWINGNNYQCLMTGSFDKTLRFWDMKNLPNQTQMAQIQLPERVYAADVLYPMAVVALANKHIKVYNLENGPTEVKDIESQLKFQIRCISIFKDKSNQNPAGFALGSIEGRVAVQYVDVANPKDNFTFKCHRSAELVNGFQEIYAVNDICFHPQHGTLVTIGSDGRYSMWDKDARTKLKTSEPHPMPLTCCDVHSSGAFLVYALGYDWSRGHEGNTQPGSKIVIHKCIEDMKPRPTKK mycbp2-rae1-1 1432204 n/a Rae1 1432207 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Pam 1432208 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1D mycbp2-rae1-2 1432204 n/a Rae1 1432207 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Pam 1432208 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B rae1-rpm_1-1 1432204 n/a Rae1 1432207 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a rpm1 1432209 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q17551:p.[Val2084Ala;Ile2087_Arg2088delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q17551:p.Arg2088Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2B, 2C npp_17-rpm_1 1432205 n/a rae1 1432210 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a rpm1 1432209 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q17551:p.[Val2084Ala;Ile2087_Arg2088delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1C rpm1vir-rae1 1432205 n/a rpm1 1432209 unspecified role unspecified role unspecified role unspecified role Q17551:p.[Val2084Ala;Ile2087_Arg2088delinsAlaAla] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag certain certain sequence position certain certain certain sequence position certain false Q17551:p.Arg2088Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false n/a rae1 1432207 unspecified role unspecified role unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2C npp_17-rpm_1-1 1432206 n/a rpm1 1432209 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a rae1 1432210 unspecified role unspecified role neutral component neutral component region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 3H