UniProt UniProt http://www.uniprot.org wright-2012-1 RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation wright-2012-2 RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation wright-2012-3 RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. mouse-retina Mus musculus retina retina retinal tissue anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wright R.N., Hong D.H., Perkins B. bperkins@mail.bio.tamu.edu C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation wright-2012-4 RpgrORF15 connects to the usher protein network through direct interactions with multiple whirlin isoforms. mouse-retina Mus musculus retina retina retinal tissue fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Wright R.N., Hong D.H., Perkins B. Interaction observed in both intact retinas and isolated photoreceptors. bperkins@mail.bio.tamu.edu C57BL/6 mouse retinas cDNA Invest. Ophthalmol. Vis. Sci. (0146-0404) 2012 Only protein-protein interactions imex curation q80vw5-12 Whirlin Whrn Dfnb31 Kiaa1526 WhirlinNT1 protein protein mouse Mus musculus Mouse MNAQLDGLSVSSSSTGSLGSAAAAAGGGGGAGLRLLSANVRQLHQALTALLSEPEREQFTHCLNAYHARRNVFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATTPYRQPAWAAPDGAGPGEVRLVSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKEGLRVGDQILRVNDKSLARVTHAEAVKALKGSKKLVLSVYSAGRIPGGYVTNHIYTWVDPQGRSTSPPSSLPQPHGSTLRQREDDRRSTLHLLQSGDEKKVNLVLGDGRSLGLTIRGGAEYGLGIYITGVDPGSEAESSGLK May be due to intron retention. q9r0x5-5 X-linked retinitis pigmentosa GTPase regulator ORF15 Rpgr protein protein mouse Mus musculus Mouse MAESESLVPDTGAVFTFGKTKFAENIPSKFWFKNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVSTDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQIGLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIPERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTELGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYEPYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSVNNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQKQQNQDTFEKVMESTPCTENEDSYEYEEMSKIKEVTVYKQYLAKGIYMIRPAEILEAFSDEEVGNGLDQVEEPRVFTDGKGLQSKQVGKESDEEIVSEKKTEVMEVADVKKIRESEENSKSDSLFDDLPDKTMNSESEDNKDIAEERRSSEQNMTFDSETELVEEPDSYMECERHSEQDSAEELEQPKLVEYSSEEKDEKDEKDDDEVETENLWYDRNCTEQETENVFRATRFFPKFDLKHDHLSGIPEEQEGPEDSEGNVVVEQVVQAQKENLEFEGDRKEAKAEAPSDVITEKEVSESERESGGEREDRSEGDGDQICEKVSLETEHLQRAQGKQERKKGKDKRARCILDMKEREEDKGWEKGSEGGDKMKRDEGNQEKRKKEMEERDAGDERSEEEEGEEEEPEEGEKEEGGEEEEGTSEDQSREDEGDRQEKEGRREGKGRQEDGREGWKEGEEQEQEEEIEEGEEEEREGEEEGGEEEGEGEGEREEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEDGEGEEDGEGEEEGEGEEEGEREEDGEGEEDGEGEEEGEGEEEGEGEEEGEGEEEGEGEGEEEGEGEWEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGEGEEEGGEDDEGEELEKKKGDITEEEEEEEEGQEGDEREREEHGSCEDDVEEDKTYDREEGEYKKAIGKVADNESQEDRKQSPKVSKINGSMKYGRHGTYSEKPITNLGKTQPSKMPMESRQLVENGLLGSERFWSDVLPLYLELK No experimental confirmation available. whrn_mouse Whirlin Whrn Dfnb31 Kiaa1526 protein protein mouse Mus musculus Mouse MNAQLDGLSVSSSSTGSLGSAAAAAGGGGGAGLRLLSANVRQLHQALTALLSEPEREQFTHCLNAYHARRNVFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATTPYRQPAWAAPDGAGPGEVRLVSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKEGLRVGDQILRVNDKSLARVTHAEAVKALKGSKKLVLSVYSAGRIPGGYVTNHIYTWVDPQGRSTSPPSSLPQPHGSTLRQREDDRRSTLHLLQSGDEKKVNLVLGDGRSLGLTIRGGAEYGLGIYITGVDPGSEAESSGLKVGDQILEVNGRSFLNILHDEAVKLLKSSRHLILTVKDVGRLPHARTTVDQTKWIASSRIGESVANSAGFPGDHTEEGTSKPGFYKGPAGSQVTLSSLGNQTRALLDDQARHLLTEQERATMMYYLAQYRGGTISVEAMVMALFELLNTHAKFSLLSEVRSIISPQDLDRFDHLVLRREIESMKARQPPGPGVGDTYSMVSYSDTGSSTGSHGTSTTVSSARERLLWLIDLMENTLDLEGTGETTQGSTNALPDVSVDDVKSPSEDLPGIKPPPPPPPLAQGHDRLLGQPRKPGREDPAPLSSAAHSGIVFSAPRNRSPPPGTAPTPGPSSAQDSPSSPIYASISHANPSSRKPLDTHLALVNQHPIGPFPRVQSPPHLKSPPAETPGAGACLPPPSPSEHPDAVGANQHFVLVEVHRPDSEPDVNEVRALPQTRTASTLSQLSDSGQTLSEDSGVDAGETEASTSGRGRQTASAKNKNGKEQPRTERTAEGANKPPGLLEPTSTLVRVRKSAATLGIAIEGGANTRQPLPRIVTIQRGGSAHNCGQLKVGHVILEVNGQTLRGKEHKEAARIIAEAFKTKERDYIDFLVTEFNVML Necessary for elongation and maintenance of inner and outer hair cell stereocilia in the organ of Corti in the inner ear mint Defects in Whrn are the cause of the phenotype whirler (wi). Mutants are characterized by deafness due to malformation of the cochlear inner and outer hair cells and by circling behavior. Stereocilia are shorter and wider than in wild-type animals and there is a decrease in the number of actin filaments in inner and outer hair cells. The number of outer hair cell stereocilia is reduced with increased spacing between them rpgr_mouse X-linked retinitis pigmentosa GTPase regulator Rpgr protein protein mouse Mus musculus Mouse MAESESLVPDTGAVFTFGKTKFAENIPSKFWFKNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVSTDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQIGLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIPERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTELGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYEPYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSVNNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQKQQNQDTFEKVMESTPCTENEDSYEYEEMSKIKEVTVYKQYLAKGIYMIRPAEILEAFSDEEVGNGLDQVEEPRVFTDGKGLQSKQVGKESDEEIVSEKKTEVMEVADVKKIRESEENSKSDSLFDDLPDKTMNSESEDNKDIAEERRSSEQNMTFDSETELVEEPDSYMECERHSEQDSAEELEQPKLVEYSSEEKDEKDEKDDDEVETENLWYDRNCTEQETENVFRATRFFPKFDLKHDHLSGIPEEQEGPEDSEGNVVVEQVVQAQKENLEFEGDRKEAKAEAPSDVITEKEAPQLSETVKPEEGEMDEEISILNVEDTVEEERKEGEKEIVEEGSIPETEGSETIDITDEKLDEVLKEEDSASLLQRALREYNENPKGHMYDRVKSSSSEILGGNDPTSKDIKKAKKISFFNRMSLTGQKLMQNTNDPLPEIKPIGDQIALQSDKKDANQNHMGQNLQDSTTPNMEGKSKSCTIL rpgr-q80vw5_12 1431455 n/a 1431459 unspecified role unspecified role prey prey n/a 1431460 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false The UniProt entry for Q9R0X5 isoform 5 (ORF15) differs in length from that given in the paper. dfnb31-rpgr 1431456 n/a 1431460 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range false region sufficient to bind sufficient binding region sufficient to bind range range range range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1431459 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false pdz1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pdz2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false ecoli Escherichia coli (strain K12) 1431473 1431470 1431474 1431469 physical association physical association false false false Fig 3B The UniProt entries for Q9R0X5 isoform 5 (ORF15) and Q80VW5 isoform 12 differ in length from those given in the paper. rpgr-dfnb31 1431457 n/a 1431461 unspecified role unspecified role prey prey n/a 1431462 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3D dfnb31-rpgr-1 1431458 n/a 1431461 unspecified role unspecified role neutral component neutral component n/a 1431460 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4