IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ p_rez_mart_nez-2010-5 F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. human-jurkat Homo sapiens jurkat Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pérez-Martínez M., Gordón-Alonso M., Cabrero J.R., Barrero-Villar M., Rey M., Mittelbrunn M., Lamana A., Morlino G., Calabia C., Yamazaki H., Shirao T., Vázquez J., González-Amaro R., Veiga E., Sánchez-Madrid F. Alzheimers - Interactions investigated in the context of Alzheimers disease Some Confocal Microscopy and FRET results have not been included as the origin of the YFP-CXCR4 construct involved is not known. Jurkat-derived human leukemic T cell line J77: [CD2+ CD3+ Ti+ CD8- CD16-] (Howard FD, et. al. "The CD3 zeta cytoplasmic domain mediates CD2-induced T cell activation." J Exp Med. 1992 Jul 1;176(1):139-45.) Drebrin GFP fusion proteins are Rattine in origin - according to Hayashi et al., 1999. CFP- Drebrin is also Rattine (Butkevich et al., 2004) Interactions involving CD3 and F-Actin have not been annotated as the exact identity of the molecules is not known. Similarly, the origin of the YFP-CXCR4 construct is not known, as the stated reference (Hernanz-Falcon et al., 2004) does not refer to the molecule in question, and the authors have not responded to a request for information. fsanchez.hlpr@salud.madrid.org J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease p_rez_mart_nez-2010-6 F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Pérez-Martínez M., Gordón-Alonso M., Cabrero J.R., Barrero-Villar M., Rey M., Mittelbrunn M., Lamana A., Morlino G., Calabia C., Yamazaki H., Shirao T., Vázquez J., González-Amaro R., Veiga E., Sánchez-Madrid F. Alzheimers - Interactions investigated in the context of Alzheimers disease Some Confocal Microscopy and FRET results have not been included as the origin of the YFP-CXCR4 construct involved is not known. Jurkat-derived human leukemic T cell line J77: [CD2+ CD3+ Ti+ CD8- CD16-] (Howard FD, et. al. "The CD3 zeta cytoplasmic domain mediates CD2-induced T cell activation." J Exp Med. 1992 Jul 1;176(1):139-45.) Drebrin GFP fusion proteins are Rattine in origin - according to Hayashi et al., 1999. CFP- Drebrin is also Rattine (Butkevich et al., 2004) Interactions involving CD3 and F-Actin have not been annotated as the exact identity of the molecules is not known. Similarly, the origin of the YFP-CXCR4 construct is not known, as the stated reference (Hernanz-Falcon et al., 2004) does not refer to the molecule in question, and the authors have not responded to a request for information. fsanchez.hlpr@salud.madrid.org J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease p_rez_mart_nez-2010-1 F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Pérez-Martínez M., Gordón-Alonso M., Cabrero J.R., Barrero-Villar M., Rey M., Mittelbrunn M., Lamana A., Morlino G., Calabia C., Yamazaki H., Shirao T., Vázquez J., González-Amaro R., Veiga E., Sánchez-Madrid F. Alzheimers - Interactions investigated in the context of Alzheimers disease Some Confocal Microscopy and FRET results have not been included as the origin of the YFP-CXCR4 construct involved is not known. Jurkat-derived human leukemic T cell line J77: [CD2+ CD3+ Ti+ CD8- CD16-] (Howard FD, et. al. "The CD3 zeta cytoplasmic domain mediates CD2-induced T cell activation." J Exp Med. 1992 Jul 1;176(1):139-45.) Drebrin GFP fusion proteins are Rattine in origin - according to Hayashi et al., 1999. CFP- Drebrin is also Rattine (Butkevich et al., 2004) Interactions involving CD3 and F-Actin have not been annotated as the exact identity of the molecules is not known. Similarly, the origin of the YFP-CXCR4 construct is not known, as the stated reference (Hernanz-Falcon et al., 2004) does not refer to the molecule in question, and the authors have not responded to a request for information. fsanchez.hlpr@salud.madrid.org J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease p_rez_mart_nez-2010-2 F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. human-jurkat Homo sapiens jurkat Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pérez-Martínez M., Gordón-Alonso M., Cabrero J.R., Barrero-Villar M., Rey M., Mittelbrunn M., Lamana A., Morlino G., Calabia C., Yamazaki H., Shirao T., Vázquez J., González-Amaro R., Veiga E., Sánchez-Madrid F. Alzheimers - Interactions investigated in the context of Alzheimers disease Some Confocal Microscopy and FRET results have not been included as the origin of the YFP-CXCR4 construct involved is not known. Jurkat-derived human leukemic T cell line J77: [CD2+ CD3+ Ti+ CD8- CD16-] (Howard FD, et. al. "The CD3 zeta cytoplasmic domain mediates CD2-induced T cell activation." J Exp Med. 1992 Jul 1;176(1):139-45.) Drebrin GFP fusion proteins are Rattine in origin - according to Hayashi et al., 1999. CFP- Drebrin is also Rattine (Butkevich et al., 2004) Interactions involving CD3 and F-Actin have not been annotated as the exact identity of the molecules is not known. Similarly, the origin of the YFP-CXCR4 construct is not known, as the stated reference (Hernanz-Falcon et al., 2004) does not refer to the molecule in question, and the authors have not responded to a request for information. fsanchez.hlpr@salud.madrid.org J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease p_rez_mart_nez-2010-3 F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Pérez-Martínez M., Gordón-Alonso M., Cabrero J.R., Barrero-Villar M., Rey M., Mittelbrunn M., Lamana A., Morlino G., Calabia C., Yamazaki H., Shirao T., Vázquez J., González-Amaro R., Veiga E., Sánchez-Madrid F. Alzheimers - Interactions investigated in the context of Alzheimers disease Some Confocal Microscopy and FRET results have not been included as the origin of the YFP-CXCR4 construct involved is not known. Jurkat-derived human leukemic T cell line J77: [CD2+ CD3+ Ti+ CD8- CD16-] (Howard FD, et. al. "The CD3 zeta cytoplasmic domain mediates CD2-induced T cell activation." J Exp Med. 1992 Jul 1;176(1):139-45.) Drebrin GFP fusion proteins are Rattine in origin - according to Hayashi et al., 1999. CFP- Drebrin is also Rattine (Butkevich et al., 2004) Interactions involving CD3 and F-Actin have not been annotated as the exact identity of the molecules is not known. Similarly, the origin of the YFP-CXCR4 construct is not known, as the stated reference (Hernanz-Falcon et al., 2004) does not refer to the molecule in question, and the authors have not responded to a request for information. fsanchez.hlpr@salud.madrid.org J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease p_rez_mart_nez-2010-4 F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. human-jurkat Homo sapiens jurkat Human T cell leukemia confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Pérez-Martínez M., Gordón-Alonso M., Cabrero J.R., Barrero-Villar M., Rey M., Mittelbrunn M., Lamana A., Morlino G., Calabia C., Yamazaki H., Shirao T., Vázquez J., González-Amaro R., Veiga E., Sánchez-Madrid F. Alzheimers - Interactions investigated in the context of Alzheimers disease Some Confocal Microscopy and FRET results have not been included as the origin of the YFP-CXCR4 construct involved is not known. Jurkat-derived human leukemic T cell line J77: [CD2+ CD3+ Ti+ CD8- CD16-] (Howard FD, et. al. "The CD3 zeta cytoplasmic domain mediates CD2-induced T cell activation." J Exp Med. 1992 Jul 1;176(1):139-45.) Drebrin GFP fusion proteins are Rattine in origin - according to Hayashi et al., 1999. CFP- Drebrin is also Rattine (Butkevich et al., 2004) Interactions involving CD3 and F-Actin have not been annotated as the exact identity of the molecules is not known. Similarly, the origin of the YFP-CXCR4 construct is not known, as the stated reference (Hernanz-Falcon et al., 2004) does not refer to the molecule in question, and the authors have not responded to a request for information. fsanchez.hlpr@salud.madrid.org J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease cxcr4_human C-X-C chemokine receptor type 4 Stromal cell-derived factor 1 receptor Fusin Leukocyte-derived seven transmembrane domain receptor LCR1 FB22 NPYRL HM89 CXCR4 Lipopolysaccharide-associated protein 3 protein protein human Homo sapiens Human MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYICDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS dreb_human Drebrin DBN1 Developmentally-regulated brain protein D0S117E protein protein human Homo sapiens Human MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD q07266-1 Drebrin Dbn1 Developmentally-regulated brain protein protein protein rat Rattus norvegicus (Rat) Rat MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDARLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQSLEAEEAKRRLKDQSIFGDQRDEEEESQMKKSESEVEEAAAIIAQRPDNPREFFRQQERVASASGGSCDAPSPFNHRPGRPYCPFIKASDSGPSSSSSSSSSPPRTPFPYITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPITEQIERALDEVTSSQPPPPPPPPPPAQEAQESAPRLDGEEVCKEAKVAAAPQVWAGCAEEPPRAQEPPLLQSSPTEDLMCTESPEQAVLAASPEPDASVTSVADAHAADTIETTTATTATTIADNVTPAAASLIDLWPGNGEEASTPQAEPRVPTPPSGAEASLAEVPLLNEAAQEPLPPVGEGCANLLNFDELPEPPATFCDPEEEAEGEPLAASQVLTMPSALEEVDQVLEQELEPEPHLLTNGETTQKEGTQQASEGYFSQSQEEEFAQSEEPCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD q16643-3 Drebrin Drebrin A DBN1 D0S117E Developmentally-regulated brain protein protein protein human Homo sapiens Human MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGRPYCPFIKASDSGPSSSSSSSSSPPRTPFPYITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD No experimental confirmation available. q16643-1 Drebrin Drebrin E Embryonic drebrin drebrin E2 DBN1 D0S117E Developmentally-regulated brain protein protein protein human Homo sapiens Human MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD dbn1-cxcr4-1 1444515 n/a 1444521 unspecified role unspecified role prey prey n/a 1444522 unspecified role unspecified role bait bait physical association physical association false false false 5A SEE - superantigen staphylococcal enterotoxin E cxcr4-dbn1-13 1444515 n/a 1444521 unspecified role unspecified role bait bait n/a 1444523 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 5B SEE-superantigen staphylococcal enterotoxin E cxcr4-dbn1-2 1444516 n/a 1444521 unspecified role unspecified role bait bait cyt_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1444522 unspecified role unspecified role prey prey human-jurkat Homo sapiens jurkat Human T cell leukemia physical association physical association false false false 1A Two higher molecular mass bands seen on Fig1A are stated to correspond to myosin II heavy chains, but they do not appear to have been identified in this publication. cxcr4-dbn1-5 1444517 n/a 1444521 unspecified role unspecified role bait bait cyt_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1444522 unspecified role unspecified role prey prey human-jurkat Homo sapiens jurkat Human T cell leukemia association association false false false 1C The authors state that "two bands were detected with an anti-drebrin polyclonal antibody, corresponding to the A and E isoforms of this protein". cxcr4-dbn1-6 1444518 n/a 1444524 unspecified role unspecified role prey prey n/a 1444525 unspecified role unspecified role prey prey n/a 1444521 unspecified role unspecified role bait bait association association false false false 1D cxcr4-dbn1-8 1444519 n/a 1444523 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1444521 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cyt_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 1G cxcr4-dbn1-9 1444520 n/a 1444523 unspecified role unspecified role neutral component neutral component n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1444521 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3C SEE-superantigen staphylococcal enterotoxin E Endogenous CXCR4 staining is shown.