IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kugler-2010-1 Regulation of Drosophila vasa in vivo through paralogous cullin-RING E3 ligase specificity receptors. drome-ovary Drosophila melanogaster ovary ovary Ovary anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kugler J.M., Woo J.S., Oh B.H., Lasko P. Paul.Lasko@mcgill.ca Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation kugler-2010-2 Regulation of Drosophila vasa in vivo through paralogous cullin-RING E3 ligase specificity receptors. drome-ovary Drosophila melanogaster ovary ovary Ovary anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kugler J.M., Woo J.S., Oh B.H., Lasko P. Paul.Lasko@mcgill.ca Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fbsp1_drome F-box/SPRY domain-containing protein 1 Fsn CG4643 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVDPVAALCNYNVLEVIFSYLELDDLSHCSQVCKSWYHFLNDENSDVWRWHCLNKLPKESLKSDLLASVSTYKTKLRAYFHAWSPNDCSRNVYIKPNGFTLHRNPVAQSTDAARGKIGFRHGRHTWEVIWEGPLGTVAVIGISTKEAALQCHGYVALLGSDDQSWGWNLVENHLLHNGDMQGSYPLLNNAPKYQVGERIRVILDCEDNTLSFEKNYEFLGVAFRGLPDKKLYPTVSAVYGNTEVSMVYLGTPLDG vasa1_drome ATP-dependent RNA helicase vasa vas Antigen Mab46F11 vasa CG46283 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSDDWDDEPIVDTRGARGGDWSDDEDTAKSFSGEAEGDGVGGSGGEGGGYQGGNRDVFGRIGGGRGGGAGGYRGGNRDGGGFHGGRREGERDFRGGEGGFRGGQGGSRGGQGGSRGGQGGFRGGEGGFRGRLYENEDGDERRGRLDREERGGERRGRLDREERGGERGERGDGGFARRRRNEDDINNNNNIVEDVERKREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAGGDGGYSNQNFGGVDVRGRGNYVGDATNVEEEEQWD gus_drome Protein gustavus gus CG2944 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGQKISGGVKTVSRNDSQSTFKPIIPRELQADFVKPARIDILLDMPPASRDLQLKHSWNSEDRSLNIFVKEDDKLTFHRHPVAQSTDCIRGKVGLTKGLHIWEIYWPTRQRGTHAVVGVCTADAPLHSVGYQSLVGSTEQSWGWDLGRNKLYHDSKNCAGVTYPAILKNDEAFLVPDKFLVALDMDEGTLSFIVDQQYLGIAFRGLRGKKLYPIVSAVWGHCEITMRYIGGLDPEPLPLMDLCRRTIRQKIGRTNLEEHIQQLQLPLSMKTYLLYKNRR cul1_drome Cullin homolog 1 Cul1 Lin-19 homolog protein cul-1 lin19 CG1877 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA q9vaq0_drome CUL5 Cullin5 Dmel\CG1401 cul-5 cul5 Dmel_CG1401 Cul5 Cul-5 Cul-5-RA CG1401 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLRAIGKRDRDIFEEVWPDKRRIVLKLLRQDTVSQREWQDLFFGVHFVCLWDEKGAAKIYDCLQQDIVEFIVQAQSQVQAQRGEQALLATYIVEWRKFFTQSNYLPLPFRQLEQSPQVKPSSSGSSSSASVSASGASTSAAAAAAATSCSGQAGKSGASTSAAAGASTSAAAAAASTSASTSSAAAAAAASGSGSSSSSTSTSKKNPTEDSPVRKLMLDSWNKHIFHDIKHRLQESAMKIVHAERNGDAYDAQLVVGVRESYVNLSSNAEDKLEIYRENFEMAYLKATVEFYRLKSAEQQQENGVLAYMKYADSKLREEEVRAKRYLEPSSFSILTYTLVNVLIVDHLNSIIAECPALIRDYETERLNLMFRLMDRVMHGVGVEPMMGDLQRHIMSAGLADMLSASEVITQDSEKYVERLLELFNKFSDLVRNAFNDDPRFLTARDIAFKTVVNDTSVFKMELPTSIANRGVKYTAPESKCPELLANYCDMLLRRTPLSKRLTSEQIDARLRDVLLVLKYVNNKDVFMRYHKVHLTRRLILGTSADSEKEEDIVEWLREVGMPADYVNRLARMFQDIKVSEDLNTQFRTSISRHDAINIKILNAGAWARCSERVSVSLPIELEDYIPDVEEFYKKKHSGRKLQWYHHMSNGTITFVNNFGRYDLDVTTFQMAVLFAWNQRQHDKISYENLRLATELPDPELRRTLWSLVAFPKIKKQILLMEPAAISSPKDFAENTMFYINQEFAIVKNGKSQRRGKLNLIGRLQLSTERSQQEDNQSIVQLRILRTQEAIIKIMKVRKRMNNAALQGELIDILKNMFLPSKKMIKEQLEWLIENKYMRRDDDDINMFIYVA vas-fsn 1432371 n/a 1432373 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432374 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1H vas-gus 1432371 n/a 1432374 unspecified role unspecified role bait bait n/a 1432375 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 1I, 1J vas-gus-2 1432371 n/a 1432374 predetermined predetermined participant predetermined competitor competitor competitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432375 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432374 unspecified role unspecified role bait bait association association false false false Fig. 1I The Vas peptide competed with the interaction with a Kd=35nM. vas-fsn-1 1432371 n/a 1432374 predetermined predetermined participant predetermined competitor competitor competitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1432374 unspecified role unspecified role bait bait n/a 1432373 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 1H gus-vas-2 1432372 n/a 1432375 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432374 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2D A Vas peptide containing an intact DINNN motif weakly interferes with this reaction fsn-lin19 1432372 n/a 1432373 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432376 predetermined predetermined participant predetermined unspecified role unspecified role prey prey physical association physical association false false false Fig. 3B gus-cul_5 1432372 n/a 1432375 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432377 predetermined predetermined participant predetermined unspecified role unspecified role prey prey physical association physical association false false false Fig. 3B