bhf-ucl humtsoe-2010-1 Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Mol. Cell. Biol. (0270-7306) 2010 imex curation Only protein-protein interactions humtsoe-2010-2 Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium confocal microscopy confocal microscopy immunostaining immunostaining Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Mol. Cell. Biol. (0270-7306) 2010 imex curation Only protein-protein interactions humtsoe-2010-3 Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Mol. Cell. Biol. (0270-7306) 2010 imex curation Only protein-protein interactions humtsoe-2010-4 Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Mol. Cell. Biol. (0270-7306) 2010 imex curation Only protein-protein interactions humtsoe-2010-5 Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Mol. Cell. Biol. (0270-7306) 2010 imex curation Only protein-protein interactions humtsoe-2010-6 Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Mol. Cell. Biol. (0270-7306) 2010 imex curation Only protein-protein interactions humtsoe-2010-7 Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Humtsoe J.O., Liu M., Malik A.B., Wary K.K. Cardiac - Interactions involving cardiac related proteins kkwary@uic.edu Mol. Cell. Biol. (0270-7306) 2010 imex curation Only protein-protein interactions lef1_human Lymphoid enhancer-binding factor 1 LEF1 T cell-specific transcription factor 1-alpha protein protein human Homo sapiens Human MPQLSGGGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNNDPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQESASGTGPRMTAAYI ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL ctnd1_human Catenin delta-1 p120 catenin Cadherin-associated Src substrate p120(cas) CTNND1 KIAA0384 protein protein human Homo sapiens Human MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI plpp3_human Phospholipid phosphatase 3 PLPP3 LPP3 Lipid phosphate phosphohydrolase 3 PAP2-beta PPAP2B Phosphatidate phosphohydrolase type 2b Phosphatidic acid phosphatase 2b Vascular endothelial growth factor and type I collagen-inducible protein protein protein human Homo sapiens Human MQNYKYDKAIVPESKNGGSPALNNNPRRSGSKRVLLICLDLFCLFMAGLPFLIIETSTIKPYHRGFYCNDESIKYPLKTGETINDAVLCAVGIVIAILAIITGEFYRIYYLKKSRSTIQNPYVAALYKQVGCFLFGCAISQSFTDIAKVSIGRLRPHFLSVCNPDFSQINCSEGYIQNYRCRGDDSKVQEARKSFFSGHASFSMYTMLYLVLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKTKTTLSLPAPAIRKEILSPVDIIDRNNHHNMM cadh5_human Cadherin-5 CDH5 Vascular endothelial cadherin 7B4 antigen protein protein human Homo sapiens Human MQRLMMLLATSGACLGLLAVAAVAAAGANPAQRDTHSLLPTHRRQKRDWIWNQMHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGTATVLVTLQDINDNFPFFTQTKYTFVVPEDTRVGTSVGSLFVEDPDEPQNRMTKYSILRGDYQDAFTIETNPAHNEGIIKPMKPLDYEYIQQYSFIVEATDPTIDLRYMSPPAGNRAQVIINITDVDEPPIFQQPFYHFQLKENQKKPLIGTVLAMDPDAARHSIGYSIRRTSDKGQFFRVTKKGDIYNEKELDREVYPWYNLTVEAKELDSTGTPTGKESIVQVHIEVLDENDNAPEFAKPYQPKVCENAVHGQLVLQISAIDKDITPRNVKFKFILNTENNFTLTDNHDNTANITVKYGQFDREHTKVHFLPVVISDNGMPSRTGTSTLTVAVCKCNEQGEFTFCEDMAAQVGVSIQAVVAILLCILTITVITLLIFLRRRLRKQARAHGKSVPEIHEQLVTYDEEGGGEMDTTSYDVSVLNSVRRGGAKPPRPALDARPSLYAQVQKPPRHAPGAHGGPGEMAAMIEVKKDEADHDGDGPPYDTLHIYGYEGSESIAESLSSLGTDSSDSDVDYDFLNDWGPRFKMLAELYGSDPREELLY pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV ctnnb1-lef-1 1444558 n/a 1444565 unspecified role unspecified role prey prey n/a 1444566 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1D, E. mouse LPP3: Q99JY8 human LPP3: O14495 ppap2b-ctnnd1-6 1444558 n/a 1444567 unspecified role unspecified role prey prey n/a 1444568 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7A, B, E, F, G The interaction was observed only in confluent cells. ctnnd1-ppap2b 1444558 n/a 1444567 unspecified role unspecified role bait bait n/a 1444568 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7D, E, F, H The interaction was observed only in confluent cells ppap2b-ctnnd1-7 1444558 n/a 1444568 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1444567 unspecified role unspecified role prey prey physical association physical association false false false Fig. 8B cdh5-ctnnb1-5 1444558 n/a 1444569 unspecified role unspecified role bait bait n/a 1444566 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7E, F pten-ctnnb1 1444558 n/a 1444566 unspecified role unspecified role prey prey n/a 1444570 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P60484:p.Cys124Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1G ctnnd1-ppap2b-1 1444559 n/a 1444567 immunostaining immunostaining unspecified role unspecified role neutral component neutral component fixed cell fixed cell n/a 1444568 immunostaining immunostaining unspecified role unspecified role neutral component neutral component fixed cell fixed cell colocalization colocalization false false false Fig. 7J The colocalization was observed only in confluent cells. The colocalization was observed at the plasma membrane. ppap2b-ctnnd1-2 1444560 n/a 1444567 unspecified role unspecified role prey prey n/a 1444568 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 8B ctnnd1-ppap2b-3 1444561 n/a 1444568 immunostaining immunostaining anti-tag immunocytochemistry anti-tag immunocytochemistry unspecified role unspecified role neutral component neutral component fixed cell fixed cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1444567 immunostaining immunostaining unspecified role unspecified role neutral component neutral component fixed cell fixed cell colocalization colocalization false false false Fig. 8H-J The cells in culture were confluent. The colocalization was observed at the plasma membrane. ppap2b-ctnnd1-3 1444562 n/a 1444568 unspecified role unspecified role bait bait c-cytoplasmic sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1444567 unspecified role unspecified role prey prey human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium physical association physical association false false false Fig. 9D ppap2b-ctnnd1-4 1444563 n/a 1444567 unspecified role unspecified role prey prey human-dermal_microvascular Human dermal microvascular endothelium cells (HDMVEC) dermal-microvascular_endothelium dermal microvascular endothelium n/a 1444568 unspecified role unspecified role bait bait c-cytoplasmic sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 9J ppap2b-ctnnd1-5 1444564 n/a 1444568 unspecified role unspecified role bait bait c-cytoplasmic required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1444567 unspecified role unspecified role prey prey physical association physical association false false false Fig. 10B