UniProt UniProt http://www.uniprot.org parisien-2009-1 A shared interface mediates paramyxovirus interference with antiviral RNA helicases MDA5 and LGP2. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Parisien J.P., Bamming D., Komuro A., Ramachandran A., Rodriguez J.J., Barber G., Wojahn R.D., Horvath C.M. Mapping of viral proteins to UniProt: Measles virus (strain Edmonston) (MeV) according Ref. 17, pmid 18579593; Mumps virus (strain Enders) (MuV) according Ref. 26 leading to (Ref 60 therein) pmid 12743296. For PIV5 (HPIV-5/SV5), HPIV-2, Hendra and Nipah proteins the UniProt virus reference strains have been used. DDX58 was mapped to human origin by sequence length, as the same authors also created the IFIH1 and LGP" constructs using the same PCR reaction these have also been mapped to human. horvath@northwestern.edu J. Virol. (0022-538X) 2009 mimix curation parisien-2009-2 A shared interface mediates paramyxovirus interference with antiviral RNA helicases MDA5 and LGP2. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Parisien J.P., Bamming D., Komuro A., Ramachandran A., Rodriguez J.J., Barber G., Wojahn R.D., Horvath C.M. Mapping of viral proteins to UniProt: Measles virus (strain Edmonston) (MeV) according Ref. 17, pmid 18579593; Mumps virus (strain Enders) (MuV) according Ref. 26 leading to (Ref 60 therein) pmid 12743296. For PIV5 (HPIV-5/SV5), HPIV-2, Hendra and Nipah proteins the UniProt virus reference strains have been used. DDX58 was mapped to human origin by sequence length, as the same authors also created the IFIH1 and LGP" constructs using the same PCR reaction these have also been mapped to human. horvath@northwestern.edu J. Virol. (0022-538X) 2009 mimix curation v_mease Non-structural protein V P/V protein protein mease Measles virus (strain Edmonston) MeV Subacute sclerose panencephalitis virus MAEEQARHVKNGLECIRALKAEPIGSLAIEEAMAAWSEISDNPGQERATCREEKAGSSGLSKPCLSAIGSTEGGAPRIRGQGPGESDDDAETLGIPPRNLQASSTGLQCYYVYDHSGEAVKGIQDADSIMVQSGLDGDSTLSGGDNESENSDVDIGEPDTEGYAITDRGSAPISMGFRASDVETAEGGEIHELLRLQSRGNNFPKLGKTLNVPPPPDPGRASTSETPIKKGHRREISLIWNGDRVFIDRWCNPMCSKVTLGTIRARCTCGECPRVCEQCRTDTGVDTRIWYHNLPEIPE dhx58_human Probable ATP-dependent RNA helicase DHX58 Probable ATP-dependent helicase LGP2 Protein D11Lgp2 homolog DHX58 D11LGP2E LGP2 RIG-I-like receptor LGP2 RIG-I-like receptor 3 protein protein human Homo sapiens Human MELRSYQWEVIMPALEGKNIIIWLPTGAGKTRAAAYVAKRHLETVDGAKVVVLVNRVHLVTQHGEEFRRMLDGRWTVTTLSGDMGPRAGFGHLARCHDLLICTAELLQMALTSPEEEEHVELTVFSLIVVDECHHTHKDTVYNVIMSQYLELKLQRAQPLPQVLGLTASPGTGGASKLDGAINHVLQLCANLDTWCIMSPQNCCPQLQEHSQQPCKQYNLCHRRSQDPFGDLLKKLMDQIHDHLEMPELSRKFGTQMYEQQVVKLSEAAALAGLQEQRVYALHLRRYNDALLIHDTVRAVDALAALQDFYHREHVTKTQILCAERRLLALFDDRKNELAHLATHGPENPKLEMLEKILQRQFSSSNSPRGIIFTRTRQSAHSLLLWLQQQQGLQTVDIRAQLLIGAGNSSQSTHMTQRDQQEVIQKFQDGTLNLLVATSVAEEGLDIPHCNVVVRYGLLTNEISMVQARGRARADQSVYAFVATEGSRELKRELINEALETLMEQAVAAVQKMDQAEYQAKIRDLQQAALTKRAAQAAQRENQRQQFPVEHVQLLCINCMVAVGHGSDLRKVEGTHHVNVNPNFSNYYNVSRDPVVINKVFKDWKPGGVISCRNCGEVWGLQMIYKSVKLPVLKVRSMLLETPQGRIQAKKWSRVPFSVPDFDFLQHCAENLSDLSLD ifih1_human Interferon-induced helicase C domain-containing protein 1 Helicase with 2 CARD domains Interferon-induced with helicase C domain protein 1 Melanoma differentiation-associated protein 5 Murabutide down-regulated protein RNA helicase-DEAD box protein 116 RIG-I-like receptor 2 IFIH1 MDA5 RH116 Clinically amyopathic dermatomyositis autoantigen 140 kDa protein protein human Homo sapiens Human MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDEENVAARASPEPELQLRPYQMEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKKASEPGKVIVLVNKVLLVEQLFRKEFQPFLKKWYRVIGLSGDTQLKISFPEVVKSCDIIISTAQILENSLLNLENGEDAGVQLSDFSLIIIDECHHTNKEAVYNNIMRHYLMQKLKNNRLKKENKPVIPLPQILGLTASPGVGGATKQAKAEEHILKLCANLDAFTIKTVKENLDQLKNQIQEPCKKFAIADATREDPFKEKLLEIMTRIQTYCQMSPMSDFGTQPYEQWAIQMEKKAAKEGNRKERVCAEHLRKYNEALQINDTIRMIDAYTHLETFYNEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED v_pi2h Non-structural protein V P/V protein protein pi2h human-parainfluenza-2-virus MAEEPTYTTEQVDELIHAGLGTVDFFLSRPIDAQSSLGKGSIPPGVTAVLTSAAEAKSKPVAAGPVKPRRKKVISNTTPYTIADNIPPEKLPINTPIPNPLLPLARPHGKMTDIDIVTGNITEGSYKGVELAKLGKQTLLTRFTSNEPVSSAGSAQDPNFKRGGANRERARGNHRREWSIAWVGDQVKVFEWCNPRCAPVTASARKFTCTCGSCPSICGECEGDH v_mumpe Non-structural protein V P/V Non-structural protein NS1 protein protein mumpe Mumps virus (strain Enders) MDQFIKQDETGDLIETGMNVANHFLSAPIQGTNSLSKASIIPGVAPVLIGNPEQKNIQHPTASHQGSKSKGRGSGVRSIIVPPSEAGNGGTQIPEPLFAQTGQGGIVTTVYQDPTIQPTGSYRSVELTKIGKERMINRFVEKPRISTPVTEFKRGAGSGCSRPDNPRGGHRREWSLSWVQGEVRVFEWCNPICSPITAAARFHSCKCGNCPAKCDQCERDYGPP v_hendh Non-structural protein V P/V/C protein protein hendh Hendra virus (isolate Horse/Autralia/Hendra/1994) MDKLDLVNDGLDIIDFIQKNQKEIQKTYGRSSIQQPSTKDRTRAWEDFLQSTSGEHEQAEGGMPKNDGGTEGRNVEDLSSVTSSDGTIGQRVSNTRAWAEDPDDIQLDPMVTDVVYHDHGGECTGHGPSSSPERGWSYHMSGTHDGNVRAVPDTKVLPNAPKTTVPEEVREIDLIGLEDKFASAGLNPAAVPFVPKNQSTPTEEPPVIPEYYYGSGRRGDLSKSPPRGNVNLDSIKIYTSDDEDENQLEYEDEFAKSSSEVVIDTTPEDNDSINQEEVVGDPSDQGLEHPFPLGKFPEKEETPDVRRKDSLMQDSCKRGGVPKRLPMLSEEFECSGSDDPIIQELEREGSHPGGSLRLREPPQSSGNSRNQPDRQLKTGDAASPGGVQRPGTPMPKSRIMPIKKGHRREVSICWDGRRAWVEEWCNPVCSRITPQPRKQECYCGECPTECSQCCHEE v_nipav Non-structural protein V P/V/C protein protein nipav Nipah virus MDKLELVNDGLNIIDFIQKNQKEIQKTYGRSSIQQPSIKDQTKAWEDFLQCTSGESEQVEGGMSKDDGDVERRNLEDLSSTSPTDGTIGKRVSNTRDWAEGSDDIQLDPVVTDVVYHDHGGECTGYGFTSSPERGWSDYTSGANNGNVCLVSDAKMLSYAPEIAVSKEDRETDLVHLENKLSTTGLNPTAVPFTLRNLSDPAKDSPVIAEHYYGLGVKEQNVGPQTSRNVNLDSIKLYTSDDEEADQLEFEDEFAGSSSEVIVGISPEDEEPSSVGGKPNESIGRTIEGQSIRDNLQAKDNKSTDVPGAGPKDSAVKEEPPQKRLPMLAEEFECSGSEDPIIRELLKENSLINCQQGKDAQPPYHWSIERSISPDKTEIVNGAVQTADRQRPGTPMPKSRGIPIKKGHRREISICWDGKRAWVEEWCNPACSRITPLPRRQECQCGECPTECFHCG v_piv5 Non-structural protein V P/V protein protein sv5 Simian virus 5 (strain W3) PIV5 Simian virus 5 MDPTDLSFSPDEINKLIETGLNTVEYFTSQQVTGTSSLGKNTIPPGVTGLLTNAAEAKIQESTNHQKGSVGGGAKPKKPRPKIAIVPADDKTVPGKPIPNPLLGLDSTPSTQTVLDLSGKTLPSGSYKGVKLAKFGKENLMTRFIEEPRENPIATSSPIDFKRGRDTGGFHRREYSIGWVGDEVKVTEWCNPSCSPITAAARRFECTCHQCPVTCSECERDT dhx58-p_v-1 1382549 n/a 1382551 unspecified role unspecified role prey prey n/a 1382552 unspecified role unspecified role bait bait physical association physical association false false false 1B, 2A and B, 3A ifih1-p_v 1382549 n/a 1382551 unspecified role unspecified role prey prey n/a 1382553 unspecified role unspecified role bait bait physical association physical association false false false 1B-E, , 2A and B. 3A dhx58-p_v-3 1382549 n/a 1382554 unspecified role unspecified role prey prey n/a 1382552 unspecified role unspecified role bait bait physical association physical association false false false 3A dhx58-p_v-5 1382549 n/a 1382552 unspecified role unspecified role bait bait n/a 1382555 unspecified role unspecified role prey prey physical association physical association false false false 3A dhx58-p_v_c 1382549 n/a 1382556 unspecified role unspecified role prey prey n/a 1382552 unspecified role unspecified role bait bait physical association physical association false false false 3A dhx58-p_v_c-2 1382549 n/a 1382552 unspecified role unspecified role bait bait n/a 1382557 unspecified role unspecified role prey prey physical association physical association false false false 3A ifih1-p_v-2 1382549 n/a 1382554 unspecified role unspecified role prey prey n/a 1382553 unspecified role unspecified role bait bait physical association physical association false false false 3A ifih1-p_v-4 1382549 n/a 1382553 unspecified role unspecified role bait bait n/a 1382555 unspecified role unspecified role prey prey physical association physical association false false false 3A ifih1-p_v_c 1382549 n/a 1382556 unspecified role unspecified role prey prey n/a 1382553 unspecified role unspecified role bait bait physical association physical association false false false 3A ifih1-p_v_c-2 1382549 n/a 1382553 unspecified role unspecified role bait bait n/a 1382557 unspecified role unspecified role prey prey physical association physical association false false false 3A ifih1-p_v-4 1382549 n/a 1382558 unspecified role unspecified role prey prey n/a 1382553 unspecified role unspecified role bait bait physical association physical association false false false 3A dhx58-p_v-4 1382549 n/a 1382558 unspecified role unspecified role prey prey n/a 1382552 unspecified role unspecified role bait bait physical association physical association false false false 3A p_v-ifih1 1382549 n/a 1382551 unspecified role unspecified role bait bait n/a 1382553 unspecified role unspecified role prey prey physical association physical association false false false 3B p_v-ifih1-1 1382549 n/a 1382555 unspecified role unspecified role bait bait n/a 1382553 unspecified role unspecified role prey prey physical association physical association false false false 3B p_v-dhx58 1382549 n/a 1382552 unspecified role unspecified role prey prey n/a 1382555 unspecified role unspecified role bait bait physical association physical association false false false 3B p_v-dhx58-1 1382549 n/a 1382551 unspecified role unspecified role bait bait n/a 1382552 unspecified role unspecified role prey prey physical association physical association false false false 3B ifih1-p_v-3 1382550 n/a 1382553 unspecified role unspecified role bait bait n/a 1382558 unspecified role unspecified role prey prey physical association physical association false false false 4 and 6A dhx58-p_v-3 1382550 n/a 1382558 unspecified role unspecified role prey prey n/a 1382552 unspecified role unspecified role bait bait physical association physical association false false false 4 and 6B