UniProt UniProt http://www.uniprot.org liu-2008e-1 The C. elegans SYS-1 protein is a bona fide beta-catenin. ecoli Escherichia coli (strain K12) pull down pull down anti tag western anti tag western blot anti tag western Liu J., Phillips B.T., Amaya M.F., Kimble J., Xu W. wxu@u.washington.edu, jekimble@wisc.edu Dev. Cell (1534-5807) 2008 Only protein-protein interactions imex curation liu-2008d-2 The C. elegans SYS-1 protein is a bona fide beta-catenin. ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu J., Phillips B.T., Amaya M.F., Kimble J., Xu W. wxu@u.washington.edu, jekimble@wisc.edu Dev. Cell (1534-5807) 2008 Only protein-protein interactions imex curation q9xvi2_caeel sys-1 T23D8.9 CELE_T23D8.9 protein protein caeel Caenorhabditis elegans MHSTGEPQRGPAGPYHHPMPYHVDQIPTTSHPSWHQAPHPGLPTPPYHPQQHPNYISMQPPPSQQQQMTPQALSTQQQQQVQQQQQRQLYSSPSPSRGPAAPAQNQKFRTEQWINNQAWPYSQSPAPPVAPSVMSYHQGDDRMSMLSVNTTMTNQFPDSQRCYSSNGSTCENVNPEMMQMNITQAAEQAIRLWFNTPDPMQRLHMAKTIRTWIRQDKFAQVDQANMPNCVQQILNIIYDGLKPQPVQLPISYYAQLWYNLLDILRRFTFLPIISPYIHQVVQMFCPRENGPQDFRELICNLISLNWQKDPHMKHCANQVFQIFNCIIMGVKNEKLRTEFAQHLKFEKLVGTLSEYFNPQVHPGMINPAIFIIFRFIISKDTRLKDYFIWNNNPHDQPPPPTGLIIKLNAVMIGSYRLIAGQNPETLPQNPELAHLIQVIIRTFDLLGLLLHDSDAIDGFVRSDGVGAITTVVQYPNNDLIRAGCKLLLQVSDAKALAKTPLENILPFLLRLIEIHPDDEVIYSGTGFLSNVVAHKQHVKDIAIRSNAIFLLHTIISKYPRLDELTDAPKRNRVCEIICNCLRTLNNFLMMWIPTPNGETKTAGPNEKQQVCKFIEIDILKKLMSCLSCEGMDTPGLLELRSTILRSFILLLRTPFVPKDGVLNVIDENRKENLIGHICAAYSWVFRQPNNTRTQSTKQQLVERTISLLLVLMEQCGAEKEVAQYSYSIDCPLNLLNGNQVKPTFIHNVLVVCDKILEHCPTRADIWTIDRPMLEGLTNHRNSDIAKAANSLLSRFPENDLLAGIFSNREYF pop1_caeel Protein pop-1 pop-1 W10C8.2 Posterior pharynx defect protein 1 protein protein caeel Caenorhabditis elegans MMADEELGDEVKVFRRDEDADDDPMISGETSEQQLADDKKEAVMEAELDGAGRNPSIDVLKSAFPKVEPMSPSFPGLMSHFSPGYSAAALPMFMPLFMNPYAAALRSPSLMFPMGAMSPTFPMFPPSPVYGAAIAAAAAKQHFENMAPLNMRAGHPMNQMGMPPYMHPSSMAPQNVDRRAQGGGKAKKDDHVKKPLNAFMWFMKENRKALLEEIGNNEKQSAELNKELGKRWHDLSKEEQAKYFEMAKKDKETHKERYPEWSARENYAVNKKKTKKRRDKSIPSENNDQKKCRARFGVNNTEMWCKFCKRKKKCEYATDRSGGSDITDSQDGRGTSGAYSSSSESPSPKANAGIALTTQQQQAAMMHTMLMQMRLGSTTGASTHVPSPLASSSAGRSPLDANASDSESDVEEEEDEQIDPTVMQQTHDMLMQESMCTI sys1-pop1 1432043 n/a sys1 1432045 unspecified role unspecified role prey prey Q9XVI2:p.Lys539Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength anti tag western anti tag western blot anti tag western certain certain sequence position certain certain certain sequence position certain false Q9XVI2:p.Ala533Leu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength anti tag western anti tag western blot anti tag western certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a pop-1 1432046 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pop1 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q10666:p.Asp9Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1432053 1432051 physical association physical association false false false Fig. 4D sys1-pop1-2 1432044 n/a sys1 1432045 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a pop1 1432046 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1432060 1432058 direct interaction direct interaction false false false Table 1 2.5A Rfree=26.1%, Rwork=21.6%