UniProt UniProt http://www.uniprot.org kidd-2005-1 A beta-catenin identified by functional rather than sequence criteria and its role in Wnt/MAPK signaling. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kidd A.R., Miskowski J.A., Siegfried K.R., Sawa H., Kimble J. jekimble@wisc.edu Cell (0092-8674) 2005 Only protein-protein interactions imex curation kidd-2005-2 A beta-catenin identified by functional rather than sequence criteria and its role in Wnt/MAPK signaling. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kidd A.R., Miskowski J.A., Siegfried K.R., Sawa H., Kimble J. jekimble@wisc.edu Cell (0092-8674) 2005 Only protein-protein interactions imex curation pop1_caeel Protein pop-1 pop-1 W10C8.2 Posterior pharynx defect protein 1 protein protein caeel Caenorhabditis elegans MMADEELGDEVKVFRRDEDADDDPMISGETSEQQLADDKKEAVMEAELDGAGRNPSIDVLKSAFPKVEPMSPSFPGLMSHFSPGYSAAALPMFMPLFMNPYAAALRSPSLMFPMGAMSPTFPMFPPSPVYGAAIAAAAAKQHFENMAPLNMRAGHPMNQMGMPPYMHPSSMAPQNVDRRAQGGGKAKKDDHVKKPLNAFMWFMKENRKALLEEIGNNEKQSAELNKELGKRWHDLSKEEQAKYFEMAKKDKETHKERYPEWSARENYAVNKKKTKKRRDKSIPSENNDQKKCRARFGVNNTEMWCKFCKRKKKCEYATDRSGGSDITDSQDGRGTSGAYSSSSESPSPKANAGIALTTQQQQAAMMHTMLMQMRLGSTTGASTHVPSPLASSSAGRSPLDANASDSESDVEEEEDEQIDPTVMQQTHDMLMQESMCTI q9xvi2_caeel sys-1 T23D8.9 CELE_T23D8.9 protein protein caeel Caenorhabditis elegans MHSTGEPQRGPAGPYHHPMPYHVDQIPTTSHPSWHQAPHPGLPTPPYHPQQHPNYISMQPPPSQQQQMTPQALSTQQQQQVQQQQQRQLYSSPSPSRGPAAPAQNQKFRTEQWINNQAWPYSQSPAPPVAPSVMSYHQGDDRMSMLSVNTTMTNQFPDSQRCYSSNGSTCENVNPEMMQMNITQAAEQAIRLWFNTPDPMQRLHMAKTIRTWIRQDKFAQVDQANMPNCVQQILNIIYDGLKPQPVQLPISYYAQLWYNLLDILRRFTFLPIISPYIHQVVQMFCPRENGPQDFRELICNLISLNWQKDPHMKHCANQVFQIFNCIIMGVKNEKLRTEFAQHLKFEKLVGTLSEYFNPQVHPGMINPAIFIIFRFIISKDTRLKDYFIWNNNPHDQPPPPTGLIIKLNAVMIGSYRLIAGQNPETLPQNPELAHLIQVIIRTFDLLGLLLHDSDAIDGFVRSDGVGAITTVVQYPNNDLIRAGCKLLLQVSDAKALAKTPLENILPFLLRLIEIHPDDEVIYSGTGFLSNVVAHKQHVKDIAIRSNAIFLLHTIISKYPRLDELTDAPKRNRVCEIICNCLRTLNNFLMMWIPTPNGETKTAGPNEKQQVCKFIEIDILKKLMSCLSCEGMDTPGLLELRSTILRSFILLLRTPFVPKDGVLNVIDENRKENLIGHICAAYSWVFRQPNNTRTQSTKQQLVERTISLLLVLMEQCGAEKEVAQYSYSIDCPLNLLNGNQVKPTFIHNVLVVCDKILEHCPTRADIWTIDRPMLEGLTNHRNSDIAKAANSLLSRFPENDLLAGIFSNREYF bar1_caeel Beta-catenin/armadillo-related protein 1 bar-1 pvl-1 spy-1 Protruding vulva protein 1 Suppressor of polyray 1 C54D1.6 protein protein caeel Caenorhabditis elegans MDLDPNLVINHDDTNLSEASFTMEQHTSSYSDIHMGSTPCTGHRKVDMWRNHNFDSGFQTMNHSEAPSIISSLHPSSHLSGMSSMADYEPIPTLSDQQKQKFDGITQNQADGQYNTVRAIPELTMLMKDQDNEVVHKAVILMQNIAKMECDPMRRQNEARIVDPRVIFTLRDLLRDKVEFPNIIRCTLGTFFHICNRQEGIDLVTRAIAEQPDIIPNLIRHIGTYPSSIYKYAILTMHSILSDKQRGGQSVIIARQQDAITHVTPWLEAEKSEKLLPVIVDLIRVLCEKNTEQKIKFVKMGGPQKLLMLLQHRVYENLLWRTTQLLKTFSNFDAPNLVAFGGRQILANLLSHGSPRLVQSTLETLRNISDVPSKIKEDLLLKSLLELVNSRNTTIRLYSAQIMSNLVANNRHNKEFMCGNNGVVILVRALTIATKEMGDLRDKEAQQMEDYIESLICTLRHLCVGHPMSDKVQAFVFRDPALFLHKLLTMRPVLLKHTLSLLLKVVSQHALLAPFRSCRIGDKGFVEQLIHILRVACTQLNVQESIEGVRVKDIIHLCIQILRWITRDQDILNEVVFFLQTPENSRMGDGHTLPIFVLQKANVEENTKSSALALIYNLMHHEQMANVLDRDDVLVKMLQNVQMQSQTHPELASLANNILKMMYEKREKTRNTLPRYNSYLESQFGHMSMTTPRSEALNSSGEVCEGAGEQWSTPLTDDTMMDSYCNSSGRDSSKPYNSPMYHSPPAMYPEYSIGPPETYLDPHATASCYPRPTPPQYNSYDRSPPVYNDLPSNPGPSSHSSDYYPSRNSRF sys1-pop1-3 1432146 n/a pop1 1432148 unspecified role unspecified role bait bait Q10666:p.Asp9Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a sys1 1432149 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false caeel Caenorhabditis elegans physical association physical association false false false Fig. 3A, 3C, 3E pop_1-sys_1-5 1432146 n/a sys1 1432149 unspecified role unspecified role prey prey n/a pop1 1432148 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 3D pop_1-bar-1 1432146 n/a bar-1 1432150 unspecified role unspecified role unspecified role unspecified role n/a pop1 1432148 unspecified role unspecified role unspecified role unspecified role Q10666:p.Asp9Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig.3A, 3C pop_1-sys_1-6 1432147 n/a sys1 1432149 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a pop1 1432148 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3B