IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ guarn_-2005-3 Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Guarné A., Brendler T., Zhao Q., Ghirlando R., Austin S., Yang W. guarnea@mcmaster.ca EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation guarn_-2005-1 Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Guarné A., Brendler T., Zhao Q., Ghirlando R., Austin S., Yang W. guarnea@mcmaster.ca EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation guarn_-2005-2 Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guarné A., Brendler T., Zhao Q., Ghirlando R., Austin S., Yang W. guarnea@mcmaster.ca EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation seqa_ecoli Negative modulator of initiation of replication seqA b0687 JW0674 protein protein ecoli Escherichia coli (strain K12) MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCGTI 37D9078CA3D7229B seqa-wt 1387994 n/a 1387997 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 2e SeqA forms filaments which are disrupted by certain mutations (see EBI-6671968, EBI-6671978, EBI-6671996). seqa-dimer 1387994 n/a 1387997 unspecified role unspecified role neutral component neutral component P0AFY8:p.Thr18Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false P0AFY8:p.Ile21Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P0AFY8:p.Ala25Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2e SeqA forms filaments which are disrupted by any of these mutations and forms, amongst other homodimers. seqa-octamer 1387994 n/a 1387997 unspecified role unspecified role neutral component neutral component P0AFY8:p.Ile21Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 8.0 direct interaction direct interaction false false false Fig. 2e SeqA forms filaments which are disrupted by this mutation and forms, amongst other homooctamers. seqa-tetramer 1387994 n/a 1387997 unspecified role unspecified role neutral component neutral component P0AFY8:p.Ile21Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P0AFY8:p.Thr18Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 2e SeqA forms filaments which are disrupted by any of these mutations and forms, amongst other homotetramers. seqa-dimer 1387995 n/a 1387997 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1b All = 0.22; Rwork = 0.22; Rfree = 0.24 2.15 A seqa-tetramer 1387995 n/a 1387997 unspecified role unspecified role neutral component neutral component region se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 1c, 2a This crystal assembly is only described in the paper but not in PDB. seqa-octomer 1387995 n/a 1387997 unspecified role unspecified role neutral component neutral component region se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 8.0 direct interaction direct interaction false false false Fig. 1d All = 0.22; Rwork = 0.22; Rfree = 0.24 2.15 A In PDB only a dimer and an octomer are assembled, however, the authors mention that the dimers form essentially filaments which could be recorded as multimers. seqa-wt 1387996 n/a 1387997 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 2d SeqA form filaments which are disrupted by certain mutations (see EBI-6671939). seqa-mut 1387996 n/a 1387997 unspecified role unspecified role neutral component neutral component P0AFY8:p.Ala25Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false P0AFY8:p.Thr18Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false P0AFY8:p.Ile21Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2d SeqA form filaments which are disrupted by any of these mutations.