IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cupp_vickery-2004-1 Crystal structure of IscA, an iron-sulfur cluster assembly protein from Escherichia coli. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Cupp-Vickery J.R., Silberg J.J., Ta D.T., Vickery L.E. jvickery@uci.edu,lvickery@uci.edu J. Mol. Biol. (0022-2836) 2004 Only protein-protein interactions imex curation isca_ecoli Iron-binding protein IscA yfhF Iron-sulfur cluster assembly protein iscA b2528 JW2512 protein protein ecoli Escherichia coli (strain K12) MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCGESFHV isca-tetramer 1457662 n/a 1457663 unspecified role unspecified role neutral component neutral component P0AAC8:p.Leu69Met mutation mutation certain certain sequence position certain certain certain sequence position certain false met38_leu69met se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false R = 0.2, Rwork = 0.2, Rfree = 0.25 2.3 A Fig.s 4 - 7