UniProt UniProt http://www.uniprot.org zhang-2003d-4 AIP1 mediates TNF-alpha-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3. bovin-pulmonary_artery Bos taurus pulmonary artery cells pulmonary_artery anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang R., He X., Liu W., Lu M., Hsieh J.T., Min W. Interactions describing ASK1 with 14-3-3 are not added because antibodies used for 14-3-3 are not specific. Origin of ASK1 inferred by mutant mapping. Origin of TRAF2 taken from the work of the contributing scientist, (Goeddel PMID:8710854) referenced in other work by this group. wang.min@rochester.edu. wang.min@rochester.edu J. Clin. Invest. (0021-9738) 2003 mimix curation zhang-2003d-2 AIP1 mediates TNF-alpha-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3. bovin-pulmonary_artery Bos taurus pulmonary artery cells pulmonary_artery anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhang R., He X., Liu W., Lu M., Hsieh J.T., Min W. Interactions describing ASK1 with 14-3-3 are not added because antibodies used for 14-3-3 are not specific. Origin of ASK1 inferred by mutant mapping. Origin of TRAF2 taken from the work of the contributing scientist, (Goeddel PMID:8710854) referenced in other work by this group. wang.min@rochester.edu. wang.min@rochester.edu J. Clin. Invest. (0021-9738) 2003 mimix curation zhang-2003d-1 AIP1 mediates TNF-alpha-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zhang R., He X., Liu W., Lu M., Hsieh J.T., Min W. Interactions describing ASK1 with 14-3-3 are not added because antibodies used for 14-3-3 are not specific. Origin of ASK1 inferred by mutant mapping. Origin of TRAF2 taken from the work of the contributing scientist, (Goeddel PMID:8710854) referenced in other work by this group. wang.min@rochester.edu. wang.min@rochester.edu J. Clin. Invest. (0021-9738) 2003 mimix curation zhang-2003d-3 AIP1 mediates TNF-alpha-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3. human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang R., He X., Liu W., Lu M., Hsieh J.T., Min W. Interactions describing ASK1 with 14-3-3 are not added because antibodies used for 14-3-3 are not specific. Origin of ASK1 inferred by mutant mapping. Origin of TRAF2 taken from the work of the contributing scientist, (Goeddel PMID:8710854) referenced in other work by this group. wang.min@rochester.edu. wang.min@rochester.edu J. Clin. Invest. (0021-9738) 2003 mimix curation f1mxh6_bovin Mitogen-activated protein kinase kinase kinase MAP3K5 protein protein bovin Bos taurus (Bovine) Bovine MSAEAGEGITFSVPPFAEGGSYRRGGAATAAEGEENQLPPPPPGSFWNVESAAAPGAGCPSATSGSSATRCRGNSGGGGGRRTTVAYVINEASQGQLVVAESEALQCLREACETVGAALETLHFGKLDFGETAVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFIPYMITPHSKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPLVQNEEQVASDMYCLVGRIYKDMFLDSNFMDTESRDHGASWFKKAFESEPTLQSGINYAVLLLASGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHLRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTSEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGKNTEEGDCEGDSLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRDCANDLLMDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGEKDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKVVRNLMESLAQGAEEPKLKWEHITTLVASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDAEGDHDERPSDQTVRRPHAVLEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVQKEKELQALLHQAIEEKDQEIKHLKLKSQPIDVPGLPGCHSSSPGTNTEDSELTDWLRENGADEDTISRFLAEDYSLMDVLYYVTRDDLKCLRLRGGMLCTLWKAITDFRDKQA e1bb77_bovin DAB2IP protein protein bovin Bos taurus (Bovine) Bovine MRSPRLTSSPIFLFQHWPQAVRDEEQRRAESPQERPGSRRSLPGSLSEKSPSMEPSATTPFRVTGFLSRRLKGSIKRTKSQPKLDRNHSFRHILPGFRSAAAAAAASAADNERSHLMPRLKESRSHESLLSPSSAVEALDLSMEEEVVIKPVHSSILGQDYCFEVTTSSGSKCFSCRSAAERDKWMENLRRAVHPNKDNSRRVEHILKLWVIEAKDLPAKKKYLCELCLDDVLYARTTGKLKTDNVFWGEHFEFHNLPPLRMVTVHLYRETDKKKKKERSSYLGLVSLPAASVAGRQFVEKWYPVVTPNPKGGKGPGPMIRIKARYQTITILPMEMYKEFAEHITNHYLGLCAALEPILSAKTKEEMASALVHILQSTGKVKDFLTDLMMSEVDRCGDNEHLIFRENTLATKAIEEYLKLVGQKYLQDALGEFIKALYESDENCEVDPSKCSAADLPEHQGNLKMCCELAFCKIINSYCVFPRELKEVFASWRQECSSRGRPDISERLISASLFLRFLCPAIMSPSLFNLLQEYPDDRTARTLTLIAKVTQNLANFAKFGSKEEYMSFMNQFLEHEWTNMQRFLLEISNPETVSNTAGFEGYIDLGRELSSLHSLLWEAVSQLEQSIVSKLGPLPRILRDVHTALSTPGSGQLAGTNDLASTPGSGSSSVSAGLQKMVIENDLSGLIDFTRLPSPTPENKDLFFVTRSSGVQPSPARSSSYSEANEPDLQMANGGKSLSMVDLQDARVLDGEAGSPAGPDALTADGQAPATQLVAGWPARAAPVSLAGLATVRRAGQTPTTPGTSEGAPGRPQLLAPLSFQNPVYQMAAGLPLSPRGLGDSGSEGHSSLSSHSNSEELAAATKLGSFSSSTAAAAPEDLGRRPGELARRQVSLTEKGGQPTVPRQNSAGPQRRIDQPPPPPPPPPPAPRGRTPPTLLSTLQYPRPSSGTLASASPDWAGPGARLRQQSSSSKGDSPELKPRAVHKQGPSPVSPNALDRTAAWLLTMNAQLLEDEGLGPDPPHRDRLRSKEELSQAEKDLAVLQDKLRISTKKLEEYETLFKCQEETTQKLVLEYQARLEEGEERLRRQQEDKDIQMKGIISRLMSVEEELKKDHAEMQAAVDSKQKIIDAQEKRIASLDAANARLMSALTQLKESMH traf2_human TNF receptor-associated factor 2 TRAF2 TRAP3 Tumor necrosis factor type 2 receptor-associated protein 3 E3 ubiquitin-protein ligase TRAF2 RING-type E3 ubiquitin transferase TRAF2 protein protein human Homo sapiens Human MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTYDGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL e1b9d9_bovin TNF receptor-associated factor TRAF2 protein protein bovin Bos taurus (Bovine) Bovine MAAASVTPPGSLDLLQPGFSKTLLGTKLEDKYLCSACKNVLRRPFQAQCGHRYCSFCLSSILSSGPQSCAACVHEGIYEEGVSILESSSAFPDNAARREVESLPAVCPSEGCSWKGTLKDYESCHEGQCPFMLTECPACKGLVRLGEKEHHLEHQCPERSLSCRHCRAPCSPADMKAHHQICPKFPLTCDGCGKKKIAREKFQDHVRACGRCRVPCRFHAVGCPEMVESEQQPEHEAQRLQEHLALLLGVLLEAGRPPGDSRLLLRPGEGALEAGTPSRVAELLQRCEALERKTATFENIVCVLNREVERVAVTAEACGRQHRLDQDRIEALSNKVQQLERSIGLKDLAMADLEQKVLEMEASTFDGVFIWKISDFSRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRAYLNGDGTGRGTHLSLFFVLMRGPHDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVGDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL dab2p_human Disabled homolog 2-interacting protein DAB2IP AF9Q34 AIP1 KIAA1743 ASK-interacting protein 1 DOC-2/DAB-2 interactive protein protein protein human Homo sapiens Human MSAGGSARKSTGRSSYYYRLLRRPRLQRQRSRSRSRTRPARESPQERPGSRRSLPGSLSEKSPSMEPSAATPFRVTGFLSRRLKGSIKRTKSQPKLDRNHSFRHILPGFRSAAAAAADNERSHLMPRLKESRSHESLLSPSSAVEALDLSMEEEVVIKPVHSSILGQDYCFEVTTSSGSKCFSCRSAAERDKWMENLRRAVHPNKDNSRRVEHILKLWVIEAKDLPAKKKYLCELCLDDVLYARTTGKLKTDNVFWGEHFEFHNLPPLRTVTVHLYRETDKKKKKERNSYLGLVSLPAASVAGRQFVEKWYPVVTPNPKGGKGPGPMIRIKARYQTITILPMEMYKEFAEHITNHYLGLCAALEPILSAKTKEEMASALVHILQSTGKVKDFLTDLMMSEVDRCGDNEHLIFRENTLATKAIEEYLKLVGQKYLQDALGEFIKALYESDENCEVDPSKCSAADLPEHQGNLKMCCELAFCKIINSYCVFPRELKEVFASWRQECSSRGRPDISERLISASLFLRFLCPAIMSPSLFNLLQEYPDDRTARTLTLIAKVTQNLANFAKFGSKEEYMSFMNQFLEHEWTNMQRFLLEISNPETLSNTAGFEGYIDLGRELSSLHSLLWEAVSQLEQSIVSKLGPLPRILRDVHTALSTPGSGQLPGTNDLASTPGSGSSSISAGLQKMVIENDLSGLIDFTRLPSPTPENKDLFFVTRSSGVQPSPARSSSYSEANEPDLQMANGGKSLSMVDLQDARTLDGEAGSPAGPDVLPTDGQAAAAQLVAGWPARATPVNLAGLATVRRAGQTPTTPGTSEGAPGRPQLLAPLSFQNPVYQMAAGLPLSPRGLGDSGSEGHSSLSSHSNSEELAAAAKLGSFSTAAEELARRPGELARRQMSLTEKGGQPTVPRQNSAGPQRRIDQPPPPPPPPPPAPRGRTPPNLLSTLQYPRPSSGTLASASPDWVGPSTRLRQQSSSSKGDSPELKPRAVHKQGPSPVSPNALDRTAAWLLTMNAQLLEDEGLGPDPPHRDRLRSKDELSQAEKDLAVLQDKLRISTKKLEEYETLFKCQEETTQKLVLEYQARLEEGEERLRRQQEDKDIQMKGIISRLMSVEEELKKDHAEMQAAVDSKQKIIDAQEKRIASLDAANARLMSALTQLKERYSMQARNGISPTNPTKLQITENGEFRNSSNC m3k5_human Mitogen-activated protein kinase kinase kinase 5 MAPK/ERK kinase kinase 5 Apoptosis signal-regulating kinase 1 MAP3K5 ASK1 MAPKKK5 MEKK5 protein protein human Homo sapiens Human MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT map3k5-dab2ip-1 1391399 n/a 1391403 unspecified role unspecified role bait bait n/a 1391404 unspecified role unspecified role prey prey n/a 1391405 unspecified role unspecified role prey prey association association false false false 3e traf2-dab2ip 1391399 n/a 1391404 unspecified role unspecified role prey prey n/a 1391406 unspecified role unspecified role bait bait physical association physical association false false false 3f dab2ip-dab2ip-3 1391399 n/a 1391407 unspecified role unspecified role prey prey n/a 1391407 unspecified role unspecified role bait bait self interaction self interaction false false false 4b map3k5-map3k5-3 1391400 n/a 1391403 unspecified role unspecified role bait bait n/a 1391407 unspecified role unspecified role prey prey physical association physical association false false false 1b; 2b; 3a; 6a; 6b and 6d dab2ip-dab2ip-2 1391400 n/a 1391407 unspecified role unspecified role prey prey n/a 1391407 unspecified role unspecified role bait bait self interaction self interaction false false false 4a map3k5-dab2ip 1391401 n/a 1391408 unspecified role unspecified role bait bait n/a 1391407 unspecified role unspecified role prey prey physical association physical association false false false Not shown traf2-map3k5-1 1391402 n/a 1391408 unspecified role unspecified role bait bait n/a 1391407 unspecified role unspecified role prey prey physical association physical association false false false 3d