mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ asada-2003-2 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-1 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-3 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-5 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-6 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-4 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-7 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-8 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-9 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. mouse-intestinal_epitheliu Mouse intestinal epithelial cells intestinal_epithelium Intestinal epithelial cells May include "microvilli". fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation asada-2003-10 ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Asada M., Irie K., Morimoto K., Yamada A., Ikeda W., Takeuchi M., Takai Y. Interaction detection: Yeast two hybrid screen. The source of Afadin inferred from the amino acid length (1829 aa.) specified by the author and the source of ADIP (mouse and rat) inferred from the libraries (mouse embryo and rat brain) used or specified by the author. The source of alpha-actinin inferred from the library used for screening (rat lung). ytakai@molbio.med.osaka-u.ac.jp rat lung and rat brain cDNA J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation afad_rat Afadin Afdn Af6 Afadin adherens junction formation factor Mllt4 Protein Af-6 protein protein rat Rattus norvegicus (Rat) Rat MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDIHSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQDYRRRESRTQDAAGPELMLPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENTELTQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYTSGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPVQNQWPNYEEKPHMHTESDHASIAIQRVTRSQEELREEKVYQLERHRVESGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGISMDLPLPPPPANQAAPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKGDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREVEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPGLSRPPLPQDYEPPSQSSAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDDEEENYVPAGPNSYSGSAGTTAGTYDAPRDTREKLSRSQDADLPGSSGAPENLTFRERQRLFSQGQDVSDKVKASRKLTELENELNTK adip_rat Afadin- and alpha-actinin-binding protein Ssx2ip Afadin DIL domain-interacting protein protein protein rat Rattus norvegicus (Rat) Rat MGDWMTVTDPVLCTENKNLSQYTSETKMSPSSLYSQQVLCSATPLSKNVHGVFSAFCTGENIEQSISYLDQELTTFGFPSLYEESKSKEAKRELSIVALLNCMNELLVLQRKNLLAQESVETQNLKLGSDMDHLQSCYAKLKEQLEASRREMISLQERDRQLQCKNRNLHQLLKNEKEEVQKLQNIIASRATQYNHDVKRKEREYNKLKERLHQLVMNKKDKNIAMDVLNYVGRVDGKRGSWRTDKTEARNEDEMYKILLNDYEYRQKQILLENAELKKVLQQMKKEMISLLSPQKKKPRERAEDSTGTVVISDVEDDAGELSRDGVWSLSCDTVREQLTNSIRKQWRILKSHVEKLDNQASKVHSEGFHEEDVISRQDHEQETEKLELEIERCKEMIKAQQQLLQQQLATACDDDTTSLLRDCYLLEEKERLKEEWSLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWVKQQFLNMTTFDHQNSENVKLFSAFSGSSDPDNLIVHPRPRQKKPHSVANGVPACTSKLAKSLPTSPSDFCPSRSCVSEHSPVSALTVTPEETKPNEVGRESTDQKWSVVSRPSSREGCYGGCSSAYTSSHVERDDLP adip_mouse Afadin- and alpha-actinin-binding protein Ssx2ip Afadin DIL domain-interacting protein protein protein mouse Mus musculus Mouse MGDWMTVTDPVLCTENKNLSQYTSETKMSPSSLYSQQVLCSSVPLSKNVHGVFGVFCTGENIEQSISYLDQELTTFGFPSLYEESKSKEAKRELNIVAVLNCMNELLVLQRKNLLAQESVETQNLKLGSDMDHLQSCYAKLKEQLETSRREMIGLQERDRQLQCKNRSLHQLLKNEKDEVQKLQNIIASRATQYNHDVKRKEREYNKLKERLHQLVMNKKDKNIAMDVLNYVGRADGKRGSWRTDKTEARNEDEMYKILLNDYEYRQKQILMENAELKKVLQQMKKEMISLLSPQKKKPRERAEDGTGTVAISDIEDDSGELSRDSVWGLSCDTVREQLTNSIRKQWRILKSHVEKLDNQASKVHSEGLNEEDVISRQDHEQETEKLELEIERCKEMIKAQQQLLQQQLATTCDDDTTSLLRDCYLLEEKERLKEEWTLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWVKQQFLNMTNFDHQNSENVKLFSAFSGSSDPDNLIVHSRPRQKKLHSVANGVPACTSKLTKSLPASPSTSDFRQTHSCVSEHSSISVLNITPEESKPSEVARESTDQKWSVQSRPSSREGCYSGCSSAFRSAHGDRDDLP actn1_rat Alpha-actinin-1 Actn1 Alpha-actinin cytoskeletal isoform Non-muscle alpha-actinin-1 F-actin cross-linking protein protein protein rat Rattus norvegicus (Rat) Rat MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHSRQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITGDELRRELPPDQAEYCIARMAPYAGPDSVPGALDYMSFSTALYGESDL myo4_yeast Myosin-4 MYO4 SHE1 YAL029C FUN22 SWI5-dependent HO expression protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFEVGTKCWYPHKEQGWIGGEVTKNDFFEGTFHLELKLEDGETVSIETNSFENDDDHPTLPVLRNPPILESTDDLTTLSYLNEPAVLHAIKKRYMNGQIYTYSGIVLIAANPFDKVDHLYSREMIQNYSSKRKDELEPHLFAIAEEAYRFMVHEKANQTVVVSGESGAGKTVSAKYIMRYFASVQESNNREGEVEMSQIESQILATNPIMEAFGNAKTTRNDNSSRFGKYLQILFDENTTIRGSKIRTYLLEKSRLVYQPETERNYHIFYQILEGLPEPVKQELHLSSPKDYHYTNQGGQPNIAGIDEAREYKITTDALSLVGINHETQLGIFKILAGLLHIGNIEMKMTRNDASLSSEEQNLQIACELLGIDPFNFAKWIVKKQIVTRSEKIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQDHVFSFIGILDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDNQPCIDLIENKLGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTKFIVSHYAVDVEYEVEGFIEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRSDDAPEEQNTEKKIMIPARLSQKKPTLGSMFKKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMVLSQLRACGVLETIRISCAGFPSRWTFDEFVQRYFLLTDYSLWSGILYNPDLPKEAIVNFCQSILDATISDSAKYQIGNTKIFFKAGMLAFLEKLRTNKMNEICIIIQKKIRARYYRLQYLQTMESIKKCQSQIRSLLVRTRVDHELKTRAAILLQTNIRALWKREYYRAAIGQIIKLQCTCKRKLILDSVNRKFMLMAAVIIQSYIRSYGHKTDYRTLKRSSILVQSAMRMQLARRRYIVLQKEVEERNIRASYGIGLLEEAIEFKNSFILNLEMLNDSYTRLTQLLQGDLSNIPSKQRQEYETIVNGYNDKISKLKTLQVEIMNTLNKKNALKERKKKQSSLIQSHMQSLAAIKGNKPSRLSDEVKSMKQELAFIENVIAQDFTTTYSANKNDKVKGLGIAGQQVKPKLVNVIRRESGNPDLLELLMDLNCYTLEVTEGYLKKVNVTEVNGDNVLGPIHVITTVVSSLVRNGLLIQSSKFISKVLLTVESIVMSLPKDETMLGGIFWLSNLSRLPAFAANQKTLYEANGGDEKDKLTLIYLNDLENETLKVFDKIYSTWLVKFMKHASAHIEIFDMVLNEKLFKNSGDEKFAKLFTFLNEFDAVLCKFQVVDSMHTKIFNDTLKYLNVMLFNDLITKCPALNWKYGYEVDRNIERLVSWFEPRIEDVRPNLIQIIQAVKILQLKISNLNEFKLLFDFWYALNPAQIQAILLKYKPANKGEAGVPNEILNYLANVIKRENLSLPGKMEIMLSAQFDSAKNHLRYDTSAITQNSNTEGLATVSKIIKLDRK E79C0FE72B041E95 she3_yeast SWI5-dependent HO expression protein 3 YBR1005 YBR130C SHE3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDQDNTQTSSSKLAPHHNIFMANLESSPTKDRNTSSQNASSSRVIESLHDQIDMLTKTNLQLTTQSQNLLSKLELAQSKESKLLENLNLLKNENENLNSIFERKNKKLKELEKDYSELSNRYNEQKEKMDQLSKLAKNSSAIEQSCSEKLQNMEVNYNSLLESQNLYRDHYSDEISKLNEKIGLLELELSNQNLNYGSDTSSNSDIELNLNKFNDSVKDLKSLETEKDSKLSKIITHSLDELNLQSWLNLYQTNENLISTFAEKMDLKDVLKRNDEKISNKGAVVQTLKKNVQTQVESNNADALSSNNAQDMLPIKMVKLRKTPNTNDSSSNGNSSNNKRRSFYTASPLLSSGSIPKSASPVLPGVKRTASVRKPSSSSSKTNVTHNNDPSTSPTISVPPGVTRTVSSTHKKKGNSMVVHGAQS 590404D2E140BB7A actn1_human Alpha-actinin-1 ACTN1 Alpha-actinin cytoskeletal isoform Non-muscle alpha-actinin-1 F-actin cross-linking protein protein protein human Homo sapiens Human MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL f1psu6_canlf AFDN protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALAPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDIENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEMILDDDECPLQIFREWPSDKGLLVFQLKRRPADYIPKKTKKHVDGKLVKGKERAEGSAYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGSLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDGDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYTEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHMDNNHSSIAIQRVTRSQEELREDKAYQLERHRIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHPSKAGTPASTLTKSGPGRWKTPAAVQPLPVAGSQPVQTDLPPPPPPPPTHYAGDFDGIPTDLPLPPPPASQTGPASAQVVAAERKKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIQELQNKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEDERRRQEEERAKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYKLPSPSPPAAAPPPPPQRTASSLQAQALSPDSLYTARLVAYHEEEEEEHCSLAGPNSYLGSAGTAVGTHDAYRDPRERLSKSHEADLPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK f1paa2_canlf SSX2IP protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MGEWMTVTDPDLSSESKNICQYTSETKMSPSSLYSQQVLCSSIPLSKNVHSFFSAFCTEENIEQSISYLDQELTTFGFPSLYEESKGKDTKRELNIVAVLNCMNELLVLQRKNLLAQENVETQNLKLGSDMDHLQNCYAKLKEQLETSRREMIGLQERDRQLQCKNRNLHQLLKNEKDEVQKLQNIIASRATQYNHDMKRKEREYNKLKERLHQLVMNKKDKKIAMEVLNYVGRADGKRGSWRTGKTEARNEDEMYKILLNDYEYRQKQILMENAELKKVLQQMKKEMISLLSPQKQKPRERADDSTGTVISDAEEDAGELSRESMWDLSCETVREQLTNSIRKQWRILKSHVEKLDNQVSKVHLEGFTDEDVISRQDHEQETEKLELEIQQCKEMIKTQQQLLQQLATACDDDTTSLLRDCYLLEEKERLKEEWSLFKEQKKNFEKERRSFTEAAIRLGLERKAFEEERASWLKQQFLNMTTFDHQNSENMKLFSAFSGSSDRDSLSVHSRPRQKKPHSVSNGTPVCTSKLTKSLPASPSTSDFCQTRSCASEHSSINVLNITPEETKPNQVGREGTNQKWSMASRPGSQEGCYSGCSLTYTNSHVEKDDLP e2qy08_canlf ACTN1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFAAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYSGPDAVPGALDYMSFSTALYGESDL afad_mouse Afadin Afdn Af6 Afadin adherens junction formation factor Mllt4 Protein Af-6 protein protein mouse Mus musculus Mouse MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKSLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDVHSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQEYRRRENRTQDATGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENAELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGPYTSGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQSQWPNYEEKPHVHTESNHSSIAIQRVTRSQEELREEKVYQLERHRVEAGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPVHYTSEFDGIPMDLPLPPPPANQAGPQSAQVAAAEWKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRSQTLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERKDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKVDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARMQDEERRRQQQLEEMRKREAEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPGLSRPPLPRDYEPPSLSSAPCAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDEEEEDYGPAGPNSYSGSAGTAVGAYDAPREAREKLTRSQDADLPGSSGAPENLTFKERQRLFSQGQDVSDKVKASRKLTELENELNTK mllt4-ssx2ip-5 1385158 n/a 1385168 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1385169 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1385183 1385181 physical association physical association false false false Fig 2A, 2B. mllt4-ssx2ip-7 1385158 n/a 1385168 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1385170 unspecified role unspecified role prey prey coiled-coil region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1385188 1385186 physical association physical association false false false Fig 2A, 2B. ssx2ip-actn1-1 1385158 n/a 1385170 unspecified role unspecified role bait bait coiled-coil region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1385171 unspecified role unspecified role prey prey physical association physical association false false false Fig 2A. myo4-she3-1 1385158 n/a 1385172 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false The sequence range for the DIL domain [1164-1419] was inferred from UniProt. n/a 1385173 unspecified role unspecified role prey prey physical association physical association false false false Fig 2B. mllt4-ssx2ip-3 1385159 n/a 1385169 unspecified role unspecified role prey prey n/a 1385168 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Text reference (p4106). ssx2ip-actn1 1385159 n/a 1385170 unspecified role unspecified role bait bait coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1385171 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1385205 1385203 physical association physical association false false false Text reference (p 4108). ssx2ip-mllt4 1385160 n/a 1385168 unspecified role unspecified role prey prey n-terminal region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1385170 unspecified role unspecified role bait bait n-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1385212 1385209 physical association physical association false false false Fig 3A. ssx2ip-actn1-2 1385160 n/a 1385174 unspecified role unspecified role prey prey n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1385170 unspecified role unspecified role bait bait coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 1385218 1385216 physical association physical association false false false Fig 8A. ssx2ip-mllt4-1 1385161 n/a 1385168 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1385170 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1385226 1385223 direct interaction direct interaction false false false Fig 3C. ssx2ip-actn1-4 1385161 n/a 1385174 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1385170 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1385233 1385229 direct interaction direct interaction false false false Fig 8C. ssx2ip-afadin-1 1385162 n/a 1385175 unspecified role unspecified role prey prey n/a 1385176 unspecified role unspecified role bait bait physical association physical association false false false Fig 3D. ssx2ip-actn1-5 1385162 n/a 1385177 unspecified role unspecified role prey prey n/a 1385176 unspecified role unspecified role bait bait physical association physical association false false false Fig 8D. ssx2ip-afadin 1385163 n/a 1385175 unspecified role unspecified role prey prey canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line n/a 1385170 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 3B. afadin-ssx2ip 1385164 n/a 1385175 unspecified role unspecified role neutral component neutral component n/a 1385176 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 5A. ssx2ip-actn1-3 1385165 n/a 1385170 unspecified role unspecified role bait bait coiled-coil domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1385174 unspecified role unspecified role prey prey physical association physical association false false false Fig 8B. mllt4-ssx2ip-8 1385166 n/a 1385178 unspecified role unspecified role neutral component neutral component n/a 1385170 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 5B, 5C. mllt4-ssx2ip-1 1385167 n/a 1385168 unspecified role unspecified role bait bait dil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1385170 unspecified role unspecified role prey prey physical association physical association false false false Text reference (p4106).