I2D I2D carr-2001-1 Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S. carrd@ohsu.edu. pB42AD-fused human testis cDNA J. Biol. Chem. (0021-9258) 2001 imex curation Only protein-protein interactions carr-2001-2 Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S. carrd@ohsu.edu. J. Biol. Chem. (0021-9258) 2001 imex curation Only protein-protein interactions carr-2001-3 Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. mouse-spermatocyte Mus musculus spermatocyte Spermatocyte fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S. carrd@ohsu.edu. J. Biol. Chem. (0021-9258) 2001 imex curation Only protein-protein interactions akap3_human A-kinase anchor protein 3 AKAP3 AKAP110 SOB1 A-kinase anchor protein 110 kDa Cancer/testis antigen 82 Fibrous sheath protein of 95 kDa Fibrousheathin I Fibrousheathin-1 Protein kinase A-anchoring protein 3 Sperm oocyte-binding protein protein protein human Homo sapiens Human MSEKVDWLQSQNGVCKVDVYSPGDNQAQDWKMDTSTDPVRVLSWLRRDLEKSTAEFQDVRFKPGESFGGETSNSGDPHKGFSVDYYNTTTKGTPERLHFEMTHKEIPCQGPRAQLGNGSSVDEVSFYANRLTNLVIAMARKEINEKIDGSENKCVYQSLYMGNEPTPTKSLSKIASELVNETVSACSRNAAPDKAPGSGDRVSGSSQSPPNLKYKSTLKIKESTKERQGPDDKPPSKKSFFYKEVFESRNGDYAREGGRFFPRERKRFRGQERPDDFTASVSEGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLLKHAKEVVSDLIDSFLRNLHSVTGTLMTDTQFVSAVKRTVFSHGSQKATDIMDAMLRKLYNVMFAKKVPEHVRKAQDKAESYSLISMKGMGDPKNRNVNFAMKSETKLREKMYSEPKSEEETCAKTLGEHIIKEGLTLWHKTQQKECKSLGFQHAAFEAPNTQRKPASDISFEYPEDIGNLSLPPYPPEKPENFMYDSDSWAEDLIVSALLLIQYHLAQGGRRDARSFVEAAGTTNFPANEPPVAPDESCLKSAPIVGDQEQAEKKDLRSVFFNFIRNLLSETIFKRDQSPEPKVPEQPVKEDRKLCERPLASSPPRLYEDDETPGALSGLTKMAVSQIDGHMSGQMVEHLMNSVMKLCVIIAKSCDASLAELGDDKSGDASRLTSAFPDSLYECLPAKGTGSAEAVLQNAYQAIHNEMRGTSGQPPEGCAAPTVIVSNHNLTDTVQNKQLQAVLQWVAASELNVPILYFAGDDEGIQEKLLQLSAAAVDKGCSVGEVLQSVLRYEKERQLNEAVGNVTPLQLLDWLMVNL rop1b_human Ropporin-1B ROPN1B Rhophilin-associated protein 1B protein protein human Homo sapiens Human MAQTDKPTCIPPELPKMLKEFAKAAIRAQPQDLIQWGADYFEALSRGETPPVRERSERVALCNWAELTPELLKILHSQVAGRLIIRAEELAQMWKVVNLPTDLFNSVMNVGRFTEEIEWLKFLALACSALGVTITKTLKIVCEVLSCDHNGGLPRIPFSTFQFLYTYIAEVDGEICASHVSRMLNYIEQEVIGPDGLITVNDFTQNPRVWLE rop1l_human Ropporin-1-like protein RSPH11 ROPN1L ASP AKAP-associated sperm protein protein protein human Homo sapiens Human MPLPDTMFCAQQIHIPPELPDILKQFTKAAIRTQPADVLRWSAGYFSALSRGDPLPVKDRMEMPTATQKTDTGLTQGLLKVLHKQCHHKRYVELTDLEQKWKNLCLPKEKFKALLQLDPCENKIKWINFLALGCSMLGGSLNTALKHLCEILTDDPEGGPARIPFKTFSYVYRYLARLDSDVSPLETESYLASLKENIDARKNGMIGLSDFFFPKRKLLESIENSEDVGH kap2_human cAMP-dependent protein kinase type II-alpha regulatory subunit PKR2 PRKAR2 PRKAR2A protein protein human Homo sapiens Human MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ rop1_mouse Ropporin-1 Ropn1 Rhophilin-associated protein 1 Rhophilin-associated 'oppo' protein protein protein mouse Mus musculus Mouse MPQTDKQVCIPPELPELLKQFTKDAIRTQPPDLIQWAAEYFGAMSRGEIPPVRERSEQIPLSNWAELTPELLKVLHSRVAGRLIIHADELAQMWKVLNLPTDLFNSVMNVGRFTEEIEWLKFLALACSSLGVTIAKTLKIVCEVLSSDHDGGPPRIPFSTFQFLYTYIAEVDGEISSSHVSRMLNYIEQEVIGPDGLIKVNDFTQNPRVRLE mint akap3_mouse A-kinase anchor protein 3 Akap3 Akap110 A-kinase anchor protein 110 kDa Protein kinase A-anchoring protein 3 protein protein mouse Mus musculus Mouse MADRVDWLQSQSGVCKVGVYSPGDNQHQDWKMDTSTDPVRVLSWLRKDLEKSTAGFQDSRFKPGESSFVEEVAYPVDQRKGFCVDYYNTTNKGSPGRLHFEMSHKENPSQGLISHVGNGGSIDEVSFYANRLTNLVIAMARKEINEKIHGAENKCVHQSLYMGDEPTPHKSLSTVASELVNETVTACSKNISSDKAPGSGDRASGSSQAPGLRYTSTLKIKESTKEGKCPDDKPGTKKSFFYKEVFESRNAGDAKEGGRSLPGDQKLFRTSPDNRPDDFSNSISQGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLMKHAKEVVSDLIDSFMKNLHGVTGSLMTDTDFVSAVKRSFFSHGSQKATDIMDAMLGKLYNVMFAKKFPENIRRARDKSESYSLISTKSRAGDPKLSNLNFAMKSESKLKENLFSTCKLEKEKTCAETLGEHIIKEGLHMWHKSQQKSPGLERAAKLGNAPQEVSFECPDPCEANPPHQPQPPENFANFMCDSDSWAKDLIVSALLLIQYHLAQGGKMDAQSFLEAAASTNFPTNKPPPPSPVVQDECKLKSPPHKICDQEQTEKKDLMSVIFNFIRNLLSETIFKSSRNCESNVHEQNTQEEEIHPCERPKTPCERPITPPAPKFCEDEEATGGALSGLTKMVANQLDNCMNGQMVEHLMDSVMKLCLIIAKSCDSPLSELGEEKCGDASRPNSAFPDNLYECLPVKGTGTAEALLQNAYLTIHNELRGLSGQPPEGCEIPKVIVSNHNLADTVQNKQLQAVLQWVAASELNVPILYFAGDDEGIQEKLLQLSATAVEKGRSVGEVLQSVLRYEKERQLDEAVGNVTRLQLLDWLMANL akap3-1 1469227 n/a 1469230 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast n/a 1469231 unspecified role unspecified role prey prey yeast Saccharomyces cerevisiae Baker's yeast physical association physical association false false false fig 1 akap3-ropn1l-1 1469227 n/a 1469232 unspecified role unspecified role prey prey n/a 1469230 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false physical association physical association false false false fig 1 akap3-prkar2a-1 1469227 n/a 1469233 unspecified role unspecified role prey prey yeast Saccharomyces cerevisiae Baker's yeast n/a 1469230 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain false yeast Saccharomyces cerevisiae Baker's yeast physical association physical association false false false Fig 1 akap3-ropn1b 1469228 n/a 1469231 unspecified role unspecified role prey prey histag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag western blot western blot Immuno blot Immuno blot n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9BZX4:p.Leu18Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1469230 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag western blot western blot Immuno blot Immuno blot n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O75969:p.Leu131Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 5 akap3-kap2-1 1469228 n/a 1469230 unspecified role unspecified role bait bait O75969:p.Leu131Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false gsttag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1469233 unspecified role unspecified role prey prey P13861:p.Leu14Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 5 akap3-ropn1-2 1469229 n/a 1469234 unspecified role unspecified role unspecified role unspecified role mouse-spermatocyte Mus musculus spermatocyte Spermatocyte n/a 1469235 unspecified role unspecified role unspecified role unspecified role mouse-spermatocyte Mus musculus spermatocyte Spermatocyte colocalization colocalization false false false