mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ mcgregor-1994-2 Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting autoradiography autoradiography McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation mcgregor-1994-1 Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. in vitro In vitro pull down pull down autoradiography autoradiography McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation mcgregor-1994-3 Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation mcgregor-1994-4 Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight identificat confirmation by molecular weight weight identificat McGregor A., Blanchard A.D., Rowe A.J., Critchley D.R. Interaction detection method: GST-pull down. The source of both alpha-actinin and vinculin specified by the author. Contact-email not provided in the publication. Biochem. J. (0264-6021) 1994 Only protein-protein interactions imex curation p05094-2 Alpha-actinin-1 Smooth muscle ACTN1 Alpha-actinin cytoskeletal isoform F-actin cross-linking protein Non-muscle alpha-actinin-1 protein protein chick Gallus gallus (Chicken) Chicken MDHHYDPQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMQAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNAWGGLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHESWTDGKEAMLQQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERQAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWEHVRQLVPRRDQALMEEHARQQQNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQQIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDCEDFRACLISMGYNMGEAEFARIMSIVDPNRMGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYNGRDAVPGALDYMSFSTALYGESDL vinc_chick Vinculin VCL VINC1 Metavinculin protein protein chick Gallus gallus (Chicken) Chicken MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVSAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVRAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNTKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAKGWLRDPNAPPGDAGEQAIRQILDEAGKAGELCAGKERREILGTCKTLGQMTDQLADLRARGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPNGGSEGEEHIRGIMSEARKVAELCEEPKERDDILRSLGEISALTAKLSDLRRHGKGDSPEARALAKQIATSLQNLQSKTNRAVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGRRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARAIAAQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPSDTPNREEVFEERAANFENHAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEHLHLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEAINQPMMMAARQLHDEARKWSSKPVTVINEAAEAGVDIDEEDDADVEFSLPSDIEDDYEPELLLMPTNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ actn1-vcl-8 1280823 n/a 1280827 unspecified role unspecified role prey prey central region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chick Gallus gallus (Chicken) Chicken n/a 1280828 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick Gallus gallus (Chicken) Chicken physical association physical association false false false Fig 3 (a-f) and 4A. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1. actn1-vcl-9 1280823 n/a 1280827 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false central region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1280828 unspecified role unspecified role bait bait region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick Gallus gallus (Chicken) Chicken physical association physical association false false false Fig 3(g-l), 4B, 5b, 5c, 6b, 6d and 6e. actn1-vcl-7 1280824 n/a 1280827 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false central region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1280828 unspecified role unspecified role prey prey region 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chick-gizzard Chicken gizzard gizzard gizzard physical association physical association false false false Table 1. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1. actn1-vcl-10 1280825 n/a 1280827 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken n/a 1280828 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken physical association physical association false false false Fig 2c. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1. actn1-vcl-11 1280826 n/a 1280827 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken n/a 1280828 unspecified role unspecified role neutral component neutral component chick Gallus gallus (Chicken) Chicken physical association physical association false false false 1280826 Fig 1. The chick alpha-actinin has multiple isoforms. The protein included in this entry is alpha-actinin 1.