IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ piccinin-2012-4 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation piccinin-2012-1 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. human-ht1080 Homo sapiens HT1080 fribrosarcoma cell line ht1080 confirmed as human with IEF of AST, LDH, NP established from the biopsy from a fibrosarcoma of a 35-year-old Caucasian man in 1972 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation piccinin-2012-2 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. human-sk-ut-1 SK-UT-1_Human uterus leiomyosarcoma (GIII) cell line sk-ut-1 SK-UT-1 Human uterus leiomyosarcoma (GIII) cell line https://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=HTB-114&Template=cellBiology Has nonsense mutation in p53 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation piccinin-2012-3 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Adenovirus-E1A/Ras transformed MEFS. The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation piccinin-2012-5 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation piccinin-2012-6 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation piccinin-2012-7 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer Adenovirus-E1A/Ras transformed MEFS. The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation piccinin-2012-8 A "Twist box" Code of p53 Inactivation: Twist box:p53 Interaction Promotes p53 Degradation. in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Piccinin S., Tonin E., Sessa S., Demontis S., Rossi S., Pecciarini L., Zanatta L., Pivetta F., Grizzo A., Sonego M., Rosano C., Dei Tos A.P., Doglioni C., Maestro R. Cancer - Interactions investigated in the context of cancer The authors confirm that the interaction between Human p53 (and mutants) and full length Twist1 were confirmed for Human, Rat and Mouse Twist1. However Twist1 mutants are of Murine origin and P300 and Twist2 are of Human origin. maestro@cro.it, spiccinin@cro.it Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation twst1_mouse Twist-related protein 1 Twist1 Twist M-twist protein protein mouse Mus musculus Mouse MMQDVSSSPVSPADDSLSNSEEEPDRQQPASGKRGARKRRSSRRSAGGSAGPGGATGGGIGGGDEPGSPAQGKRGKKSAGGGGGGGAGGGGGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD cite1_human Cbp/p300-interacting transactivator 1 CITED1 MSG1 Melanocyte-specific protein 1 protein protein human Homo sapiens Human MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMHLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSDPVDEEVLMSLVVELGLDRANELPELWLGQNEFDFTADFPSSC twst2_human Twist-related protein 2 Dermis-expressed protein 1 TWIST2 DERMO1 BHLHA39 Class A basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHERLSYAFSVWRMEGAWSMSASH twst1_human Twist-related protein 1 H-twist TWIST1 TWIST BHLHA38 Class A basic helix-loop-helix protein 38 protein protein human Homo sapiens Human MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD csk21_human Casein kinase II subunit alpha CSNK2A1 CK2A1 protein protein human Homo sapiens Human MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ twist1-tp53-4 1317952 n/a 1317960 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false twist_box required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1317961 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c_terminal_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false 4B/E, S4D twist1-cited1 1317952 n/a 1317962 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1317960 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false S3I tp53-twist1-7 1317952 n/a 1317960 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P26687:p.Arg186Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P26687:p.His184Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P26687:p.Ser188Leu mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false twist_box required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1317961 unspecified role unspecified role bait bait c_terminal_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 5C, S4A tp53-twist1-8 1317952 n/a 1317960 unspecified role unspecified role prey prey twist_box required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1317961 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 4C tp53-twist2 1317952 n/a 1317961 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1317963 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false S4C twist1-tp53-1 1317953 n/a 1317964 unspecified role unspecified role bait bait n/a 1317961 unspecified role unspecified role prey prey physical association physical association false false false 4A tp53-mdm2-5 1317953 n/a 1317965 unspecified role unspecified role prey prey n/a 1317961 unspecified role unspecified role bait bait physical association physical association false false false 6E, S5I. The increase in the overall levels of p53 detected after Twist1 knockdown correlated with a slight but consistent decrease (∼30%) in the amount of p53 complexed to MDM2. twist1-tp53-2 1317954 n/a 1317961 unspecified role unspecified role prey prey n/a 1317964 unspecified role unspecified role bait bait physical association physical association false false false 4A twist1-tp53-3 1317955 n/a 1317966 unspecified role unspecified role prey prey n/a 1317960 unspecified role unspecified role bait bait physical association physical association false false false 4A tp53-twist1-9 1317956 n/a 1317961 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1317964 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4B tp53-mdm2 1317957 n/a 1317961 unspecified role unspecified role bait bait P04637:p.Ser392Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P04637:p.Ser392Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false n/a 1317965 unspecified role unspecified role prey prey physical association physical association false false false 6D tp53-twist1-6 1317958 n/a 1317960 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1317961 unspecified role unspecified role bait bait physical association physical association false false false S4B csnk2a1-tp53-1 1317959 n/a 1317960 inhibitor inhibitor neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1317961 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1317967 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false S5C Phosphorylation at Ser392 was attenuated in the presence of recombinant Twist1.