MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint brand-2012-3 A stomatin dimer modulates the activity of acid-sensing ion channels. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Brand J., Smith ES., Schwefel D., Lapatsina L., Poole K., Omerbašić D., Kozlenkov A., Behlke J., Lewin GR., Daumke O. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation brand-2012-1 A stomatin dimer modulates the activity of acid-sensing ion channels. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Brand J., Smith ES., Schwefel D., Lapatsina L., Poole K., Omerbašić D., Kozlenkov A., Behlke J., Lewin GR., Daumke O. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation brand-2012-2 A stomatin dimer modulates the activity of acid-sensing ion channels. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Brand J., Smith ES., Schwefel D., Lapatsina L., Poole K., Omerbašić D., Kozlenkov A., Behlke J., Lewin GR., Daumke O. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation brand-2012-4 A stomatin dimer modulates the activity of acid-sensing ion channels. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Brand J., Smith ES., Schwefel D., Lapatsina L., Poole K., Omerbašić D., Kozlenkov A., Behlke J., Lewin GR., Daumke O. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation brand-2012-5 A stomatin dimer modulates the activity of acid-sensing ion channels. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Brand J., Smith ES., Schwefel D., Lapatsina L., Poole K., Omerbašić D., Kozlenkov A., Behlke J., Lewin GR., Daumke O. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation brand-2012-6 A stomatin dimer modulates the activity of acid-sensing ion channels. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Brand J., Smith ES., Schwefel D., Lapatsina L., Poole K., Omerbašić D., Kozlenkov A., Behlke J., Lewin GR., Daumke O. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation brand-2012-7 A stomatin dimer modulates the activity of acid-sensing ion channels. human-hek-10 Homo sapiens hek-10 hek-10 bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Brand J., Smith ES., Schwefel D., Lapatsina L., Poole K., Omerbašić D., Kozlenkov A., Behlke J., Lewin GR., Daumke O. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation stom_mouse Stomatin Stom Epb7.2 Epb72 Protein 7.2b Erythrocyte band 7 integral membrane protein Erythrocyte membrane protein band 7.2 protein protein mouse Mus musculus Mouse MSDKRQSSHVQSQRIPESFRENSKTELGACGWILVAASFFFVIITFPISIWICIKIVKEYERVIIFRLGRILQGGAKGPGLFFILPCTDSLIKVDMRTISFDIPPQEVLTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNALGTKNLSQILSDREEIAHHMQSTLDDATDDWGIKVERVEIKDVKLPVQLQRAMAAEAEAAREARAKVIAAEGEMNASRALKEASMVITESPAALQLRYLQTLTTIAAEKNSTIVFPLPVDMLQGIMGSNH mint Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating homomint asic3_rat Acid-sensing ion channel 3 Asic3 Accn3 Amiloride-sensitive cation channel 3 Dorsal root ASIC Drasic protein protein rat Rattus norvegicus (Rat) Rat MKPRSGLEEAQRRQASDIRVFASSCTMHGLGHIFGPGGLTLRRGLWATAVLLSLAAFLYQVAERVRYYGEFHHKTTLDERESHQLTFPAVTLCNINPLRRSRLTPNDLHWAGTALLGLDPAEHAAYLRALGQPPAPPGFMPSPTFDMAQLYARAGHSLEDMLLDCRYRGQPCGPENFTVIFTRMGQCYTFNSGAHGAELLTTPKGGAGNGLEIMLDVQQEEYLPIWKDMEETPFEVGIRVQIHSQDEPPAIDQLGFGAAPGHQTFVSCQQQQLSFLPPPWGDCNTASLDPDDFDPEPSDPLGSPRPRPSPPYSLIGCRLACESRYVARKCGCRMMHMPGNSPVCSPQQYKDCASPALDAMLRKDTCVCPNPCATTRYAKELSMVRIPSRASARYLARKYNRSESYITENVLVLDIFFEALNYEAVEQKAAYEVSELLGDIGGQMGLFIGASLLTILEILDYLCEVFQDRVLGYFWNRRSAQKRSGNTLLQEELNGHRTHVPHLSLGPRPPTTPCAVTKTLSASHRTCYLVTRL stom-1 1319015 stom_mouse Stomatin 1319022 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P54116:p.Val197Pro mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false f2d mint stom-1 1319015 stom_mouse Stomatin 1319022 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P54116:p.Val197Pro mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2d mint stom-1 1319016 stom_mouse Stomatin 1319022 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false mint f2a t1 f6a f6b stom-1 1319017 stom_mouse Stomatin 1319022 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P54116:p.Val197Pro mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false 1319017 f2c mint stom-asic3 1319018 stom_mouse Stomatin 1319022 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tandem tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false accn3_rat ASIC3 1319023 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f3b homomint mint asic3-stom 1319019 stom_mouse Stomatin 1319022 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false accn3_rat ASIC3 1319023 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false homomint mint sf5 asic3-stom-1 1319020 stom_mouse Stomatin 1319022 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false accn3_rat ASIC3 1319023 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false sf4 homomint mint stom-stom 1319021 stom_mouse Stomatin 1319022 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P54116:p.Val197Pro mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false stom_mouse Stomatin 1319022 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P54116:p.Val197Pro mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false sf2 homomint mint