IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ soares-2012-4 The Mitosis and Neurodevelopment Proteins NDE1 and NDEL1 Form Dimers, Tetramers, and Polymers with a Folded Back Structure in Solution. ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Soares D.C., Bradshaw N.J., Zou J., Kennaway C.K., Hamilton R.S., Chen Z.A., Wear M.A., Blackburn E.A., Bramham J., Bottcher B., Millar J.K., Barlow P.N., Walkinshaw M.D., Rappsilber J., Porteous D.J. Cross-linking agent - bis(sulfosuccinimidyl)suberate (BS3). Only cross-links described in the main text have been included as features on the interacting molecules. Dinesh.Soares@ed.ac.uk, Nicholas.Bradshaw@uni-duesseldorf.de J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation soares-2012-3 The Mitosis and Neurodevelopment Proteins NDE1 and NDEL1 Form Dimers, Tetramers, and Polymers with a Folded Back Structure in Solution. ecoli Escherichia coli (strain K12) comigration in sds comigration in sds page comigration in sds sequence tag sequence tag identification sequence tag Soares D.C., Bradshaw N.J., Zou J., Kennaway C.K., Hamilton R.S., Chen Z.A., Wear M.A., Blackburn E.A., Bramham J., Bottcher B., Millar J.K., Barlow P.N., Walkinshaw M.D., Rappsilber J., Porteous D.J. Dinesh.Soares@ed.ac.uk, Nicholas.Bradshaw@uni-duesseldorf.de J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation soares-2012-1 The Mitosis and Neurodevelopment Proteins NDE1 and NDEL1 Form Dimers, Tetramers, and Polymers with a Folded Back Structure in Solution. ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Soares D.C., Bradshaw N.J., Zou J., Kennaway C.K., Hamilton R.S., Chen Z.A., Wear M.A., Blackburn E.A., Bramham J., Bottcher B., Millar J.K., Barlow P.N., Walkinshaw M.D., Rappsilber J., Porteous D.J. Dinesh.Soares@ed.ac.uk, Nicholas.Bradshaw@uni-duesseldorf.de J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation soares-2012-2 The Mitosis and Neurodevelopment Proteins NDE1 and NDEL1 Form Dimers, Tetramers, and Polymers with a Folded Back Structure in Solution. ecoli Escherichia coli (strain K12) light scattering light scattering predetermined predetermined participant predetermined Soares D.C., Bradshaw N.J., Zou J., Kennaway C.K., Hamilton R.S., Chen Z.A., Wear M.A., Blackburn E.A., Bramham J., Bottcher B., Millar J.K., Barlow P.N., Walkinshaw M.D., Rappsilber J., Porteous D.J. Dinesh.Soares@ed.ac.uk, Nicholas.Bradshaw@uni-duesseldorf.de J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation ndel1_human Nuclear distribution protein nudE-like 1 NDEL1 Mitosin-associated protein 1 EOPA MITAP1 NUDEL protein protein human Homo sapiens Human MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGAVNGFDPAPPPPGLGSSRPSSAPGMLPLSV nde1_human Nuclear distribution protein nudE homolog 1 NDE1 NUDE protein protein human Homo sapiens Human MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC ndel1-1 1328072 n/a 1328076 unspecified role unspecified role neutral component neutral component lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false ser-95 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false tyr-114 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-82 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328076 unspecified role unspecified role neutral component neutral component lys-20 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-25 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false ser-90 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-80 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 1328089 1328087 1328090 1328081 1328091 1328082 1328093 1328083 1328094 1328086 1328095 1328085 1328096 1328080 physical association physical association false false false Fig. 2B ndel1-ndel1-3 1328072 n/a 1328076 unspecified role unspecified role neutral component neutral component lys-29 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-80 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ser-90 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-113 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-29 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328076 unspecified role unspecified role neutral component neutral component tyr-114 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-82 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false ser-95 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328076 unspecified role unspecified role neutral component neutral component lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false ser-90 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-80 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-20 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328076 unspecified role unspecified role neutral component neutral component tyr-114 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false ser-95 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-27 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-82 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 1328108 1328104 1328110 1328101 1328111 1328100 1328112 1328103 1328113 1328106 1328115 1328099 1328122 1328105 1328124 1328117 1328125 1328116 1328126 1328118 1328128 1328121 1328129 1328119 physical association physical association false false false Fig. 2D, Supp. Table 2. nde1-1 1328072 n/a 1328077 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-170 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-315 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328077 unspecified role unspecified role neutral component neutral component lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-270 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-315 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-270 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 1328138 1328136 1328139 1328133 1328141 1328135 1328142 1328134 physical association physical association false false false Fig. 2D, Supplemental Table 2 nde1-nde1-3 1328072 n/a 1328077 unspecified role unspecified role neutral component neutral component lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-315 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-170 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328077 unspecified role unspecified role neutral component neutral component lys-270 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-315 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-270 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-112 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-26 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-81 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328077 unspecified role unspecified role neutral component neutral component lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-315 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-270 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-170 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-81 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-79 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328077 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lys-270 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-315 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-270 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 1328151 1328148 1328154 1328149 1328156 1328146 1328157 1328145 1328162 1328152 1328166 1328159 1328169 1328158 1328170 1328153 1328171 1328160 1328174 1328163 1328175 1328164 1328176 1328168 1328177 1328167 physical association physical association false false false Fig. 2D, Supplemental Table 2 nde1-ndel1-2 1328072 n/a 1328076 unspecified role unspecified role neutral component neutral component lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-17 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 n/a 1328077 unspecified role unspecified role neutral component neutral component lys-107 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false lys-170 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 1328183 1328180 1328184 1328181 physical association physical association false false false Fig. 6, Supp. Table 2 nde1-ndel1-1 1328072 n/a 1328076 unspecified role unspecified role neutral component neutral component lys-108 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 n/a 1328077 unspecified role unspecified role neutral component neutral component ser-196 spdp cross linker spdp cross linker N -Succinimidyl 3-(2-pyridyldithio)-propionate certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 1328189 1328187 physical association physical association false false false Fig. 6 and Supp Table 2 ndel1-ndel1-2 1328073 N/A 1328076 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1328076 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 2D nde1-nde1-2 1328073 n/a 1328077 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2D ndel1-8 1328073 N/A 1328076 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2D ndel1-ndel1 1328074 n/a 1328076 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1328076 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 2A nde1-nde1 1328074 n/a 1328077 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1328077 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 2A ndel1-ndel1-1 1328075 n/a 1328076 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1328076 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 2B nde1-nde1-1 1328075 n/a 1328077 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1328077 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 2B