IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rajagopala-2012-1 Studying protein complexes by the yeast two-hybrid system. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast two hybrid array two hybrid array predetermined predetermined participant predetermined Rajagopala S.V., Sikorski P., Harry Caufield J., Tovchigrechko A., Uetz P. rajgsv@gmail.com, peter@uetz.us Methods (1046-2023) 2012 Only protein-protein interactions imex curation dpo3a_ecoli DNA polymerase III subunit alpha dnaE polC b0184 JW0179 protein protein ecoli Escherichia coli (strain K12) MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDFTNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLISKAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEEHFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVAIHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEYFLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQMGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLNPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLGHPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNAGKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIINWALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQPDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYGIILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAELAMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDLRGLIGSEQVELEFD dpo3x_ecoli DNA polymerase III subunit tau dnaX dnaZ dnaZX b0470 JW0459 DNA polymerase III subunit gamma protein protein ecoli Escherichia coli (strain K12) MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI hold_ecoli DNA polymerase III subunit psi holD b4372 JW4334 protein protein ecoli Escherichia coli (strain K12) MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVANDLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSHCNSWRLGTDEPLSLEGAQVASPALTDLRANPTARAALWQQICTYEHDFFPRND holc_ecoli DNA polymerase III subunit chi holC b4259 JW4216 protein protein ecoli Escherichia coli (strain K12) MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK 7FEFFC359B28AA88 asnb_ecoli Asparagine synthetase B [glutamine-hydrolyzing] asnB b0674 JW0660 protein protein ecoli Escherichia coli (strain K12) MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNAILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVASEMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALEDSVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK trmd_ecoli tRNA (guanine-N(1)-)-methyltransferase trmD b2607 JW2588 M1G-methyltransferase tRNA [GM37] methyltransferase protein protein ecoli Escherichia coli (strain K12) MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSELATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVLGHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA perr_ecoli HTH-type transcriptional regulator PerR perR Peroxide resistance protein PerR JW0244 b0254 protein protein ecoli Escherichia coli (strain K12) MKLLAKAPLNLLRAFEAAGRTGAFALAASELELSPSAISHAIRKLENLLDVRLFQRSTREITLTKEGEILLEHIQRGFNELQQGLALVTADESRPLRLHTAPSFAHQWLLPRLGKFIRENPSIDLRLSASTEYARFEQDDFDLDIVYGEPRPSPYEKIPLAVEELTPLCSPQLAERLKKPEDLYALTLIQCDVQLYQWKGWFEANKMTPPNNYGLRFDRSFMAIAAAVDGLGVVLESKLLAEREIASGKLVCPLVNSTSEIHYIGHYLVFPQHQHMHSALDVFKTWLLNELNLGKIR mntr_ecoli Transcriptional regulator MntR ybiQ Manganese transport regulator mntR b0817 JW0801 protein protein ecoli Escherichia coli (strain K12) MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVELISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLAQESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK yhjb_ecoli Putative HTH-type transcriptional regulator YhjB yhjB b3520 JW3488 protein protein ecoli Escherichia coli (strain K12) MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLESYPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVPRDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNKEIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS yjhc_ecoli Uncharacterized oxidoreductase YjhC yjhC b4280 JW5769 protein protein ecoli Escherichia coli (strain K12) MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIARELQCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVDMVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSWKKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLEFPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQEEDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDLLNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM hycg_ecoli Formate hydrogenlyase subunit 7 Hydrogenase-3 component G hycG hevG b2719 JW2689 protein protein ecoli Escherichia coli (strain K12) MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQLGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR metn_ecoli Methionine import ATP-binding protein MetN metN abc b0199 JW0195 protein protein ecoli Escherichia coli (strain K12) MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGKSTLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGNVALPLELDNTPKDEVKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPKVLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGELIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQSVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHHVKVEVLGYV dnae-dnax 1328734 n/a 1328735 unspecified role unspecified role bait bait n/a 1328736 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1b, c hold-dnax 1328734 n/a 1328737 unspecified role unspecified role bait bait n/a 1328736 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1b, c hold-holc 1328734 n/a 1328737 unspecified role unspecified role bait bait n/a 1328738 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1b, c holc-hold 1328734 n/a 1328737 unspecified role unspecified role prey prey n/a 1328738 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1b, c hold-hold 1328734 n/a 1328737 unspecified role unspecified role prey prey n/a 1328737 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1b, c asnb-asnb 1328734 n/a 1328739 unspecified role unspecified role bait bait n/a 1328739 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1b, c trmd-trmd 1328734 n/a 1328740 unspecified role unspecified role prey prey n/a 1328740 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1b, c perr-perr 1328734 n/a 1328741 unspecified role unspecified role prey prey n/a 1328741 unspecified role unspecified role bait bait physical association physical association false false false Fig.1d mntr-yhjb 1328734 n/a 1328742 unspecified role unspecified role unspecified role unspecified role n/a 1328743 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig.1d mntr-yjhc 1328734 n/a 1328744 unspecified role unspecified role unspecified role unspecified role n/a 1328742 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig.1d hycg-hycg 1328734 n/a 1328745 unspecified role unspecified role prey prey n/a 1328745 unspecified role unspecified role bait bait physical association physical association false false false Fig.1d mntr-hycg 1328734 n/a 1328745 unspecified role unspecified role unspecified role unspecified role n/a 1328742 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig.1d yjhc-yjhc 1328734 n/a 1328744 unspecified role unspecified role prey prey n/a 1328744 unspecified role unspecified role bait bait physical association physical association false false false Fig.1d perr-mntr 1328734 n/a 1328741 unspecified role unspecified role unspecified role unspecified role n/a 1328742 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig.1d mntr-mntr 1328734 n/a 1328742 unspecified role unspecified role prey prey n/a 1328742 unspecified role unspecified role bait bait physical association physical association false false false Fig.1d hycg-metn 1328734 n/a 1328746 unspecified role unspecified role unspecified role unspecified role n/a 1328745 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig.1d metn-mntr 1328734 n/a 1328742 unspecified role unspecified role unspecified role unspecified role n/a 1328746 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig. 1D metn-yjhc 1328734 n/a 1328746 unspecified role unspecified role unspecified role unspecified role n/a 1328744 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig. 1D