IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ russo-2012-1 Natural intracellular peptides can modulate the interactions of mouse brain proteins and thimet oligopeptidase with 14-3-3ε and calmodulin. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Russo L.C., Asega A.F., Castro L.M., Negraes P.D., Cruz L., Gozzo F.C., Ulrich H., Camargo A.C., Rioli V., Ferro E.S. eferro@usp.br Proteomics (1615-9853) 2012 Only protein-protein interactions imex curation thop1_rat Thimet oligopeptidase Thop1 Endo-oligopeptidase A Endopeptidase 24.15 PZ-peptidase Soluble metallo-endopeptidase protein protein rat Rattus norvegicus (Rat) Rat MKPPAACAGDVVDTVSPCSTVNHLRWDLSAQQIRALTTQLIEQTKCVYDRVGAQDFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPNKDIRAASTEADKKLSEFDVEMSMRQDVYQRVVWLQEKIPKDSLKPEAARYLERLIKLGRRNGLHLPQDTQEKIKNIKKRLSLLCIDFNKNLNEDTTFLPFTREELGGLPEDFLNSLEKTEDGKLKVTLKYPHYFPLLKKCHVPETRRLLEEAFNCRCKEENCAILKELVSLRAQKSNLLGFRTHADYVLEMNMAKTSQTVATFLDELARKLKPLGEQERAVILELKEAECAKRGLPFDGRIHAWDMRYYMNQVEETRYRVDQNLLKEYFPMQVVTRGLLAIYQELLGLTFTLEEGAAAWHEDVRLYSVRDAASGEEIGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQLAIAAMVANFTKPTPDVPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEKEPLMRMSQHYRTGGEAPEDLLEKLIKSRQANAGLFNLRQIVLAKVDQVLHTQTDVDPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLSPKVGMDYRTSILRPGGSEDASTMLKQFLGRDPKQDAFLLSKGLQVEGCEPPAC 1433e_rat 14-3-3 protein epsilon Ywhae Mitochondrial import stimulation factor L subunit protein protein rat Rattus norvegicus (Rat) Rat MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK ywhae-thop1 1322333 n/a 1322334 unspecified role unspecified role prey prey purified purified P24155:p.His473Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1322335 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false table 2 Peptide VFD-7 (VFDVELL) disrupts the interactions in a concentration-dependent manner. Peptides AVA-9 (AVAIKAMAK) and SAM-12 (SAMTEEAAVAIK) disrupt the interaction at concentrations of 10 and 1 microM, respectively. calm-thop1 1322333 n/a 1322336 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) n/a 1322334 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false fig 4a Peptide VFD-7 (VFDVELL) disrupts the interactions in a concentration-dependent manner. calm1-thop1 1322333 n/a 1322334 unspecified role unspecified role prey prey purified purified P24155:p.His473Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1322336 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false table 2 Peptide VFD-7 (VFDVELL) disrupts the interactions in a concentration-dependent manner. Peptide AVA-9 (AVAIKAMAK) disrupts the interaction at concentration of 10 microM.