mpidb The Microbial Protein Interaction Database li-2012f-2 Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li F., Alvarez-Martinez C., Chen Y., Choi K.J., Yeo H.J., Christie P.J. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation li-2012f-1 Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding. ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Li F., Alvarez-Martinez C., Chen Y., Choi K.J., Yeo H.J., Christie P.J. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation q5g3n6_entfl pcfF DAI13_16755 protein protein entfl Enterococcus faecalis Streptococcus faecalis MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGININQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS q5g3p5_entfl prgJ EU507_13880 protein protein entfl Enterococcus faecalis Streptococcus faecalis MHIVEDEFSKCYKLGEVDYEIAVEEDQLNTVMGYAEGLNTLDKNSRYQLLVVNKREEESLMEDVLLAYQGDHLDNYRQEINEIITKQYEQDERNFKIEKYAIFTTKSSSMKQANKELEGIMQNFANRFEANQIKLEVTPMTGLERLSLFSYFLRHGQYFGTSYQDIAVSGLTSKAFIVPSKIKFPNNKAYFRLGENYAAVLTIQQYPKYLEDKLIKELCATGRELAISIHARPYDMMEARKAIQGTKTLNDVAIQKQQKQNFRSGVSEDAISGEAKEIQETTNALMDEIKNNGQKLFNGLFSVFLIAETEKELLEAVESVKNVGATWQVVFDTVDNYKEEALNTILPIGKPYLDVEMSYMRDLTTTNIATQVPFTNVELQSPTGQFYGRNQLTKNMITIDRKKDLITPSGLIFGTSGSGKGMATKWEILSTRLKFPKDRFLIVDPESEYLPIGKELDAEILDISTGTKNHLNILGMVDKQLLDEEDQELDLVKEKSNLLSTMFESLLKSYTDVDAGLVDRVTLLTYERFENKETEPTLVDWFRILKEQTDEGAKELANKVETYCVGSQDIFAHQTNIDLTAPFIVFNTKKLDDRLKKFAMKVILDQLWKQVVSGQGKVTSRLYFDELQVNFTTEEEAEWFLNLWARIRKYGAVTTGITQNPTTLLDSKAGQKMIANSEFLILLRQKPIDSQRLVEILSLTPALKKYINDRAPQGTGLISAGGVVVPFENPIPKETHLFEIMNTDA q7x5p3_entfl pcfG protein protein entfl Enterococcus faecalis Streptococcus faecalis MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPYEEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVMELTGGKYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQTHTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSLSKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYGYGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVMQTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPELIQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPKL q5g3n9_entfl pcfC protein protein entfl Enterococcus faecalis Streptococcus faecalis MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFSVGVLGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKLQKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTFNVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADMLRHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIRTDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLIDEFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSARAGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRADELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV prgj-pcff 1339772 not-defined-269153206 1339774 unspecified role unspecified role prey prey not-defined-1832963351 1339775 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3 pcff-prgj 1339772 not-defined-1946318476 1339775 unspecified role unspecified role prey prey region Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-2025652454 1339774 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig. 3 pcfg-prgj 1339772 not-defined-1871552973 1339775 unspecified role unspecified role prey prey q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-343993462 1339776 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 3 pcfc-prgj 1339772 not-defined-196467529 1339775 unspecified role unspecified role prey prey q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-299815031 1339777 unspecified role unspecified role bait bait q5g3n9-- Q5G3N9-- gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3 prgj-pcfg-1 1339772 not-defined-1526979645 1339776 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) not-defined-1350282498 1339775 unspecified role unspecified role bait bait q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3 prgj-pcfc-1 1339772 not-defined-1925378275 1339775 unspecified role unspecified role bait bait q5g3p5-- Q5G3P5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1190946819 1339777 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false table 3 prgj-prgj-1 1339773 not-defined-2027190568 1339775 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 2A