mpidb The Microbial Protein Interaction Database takekawa-2012-1 Characterization of PomA mutants defective in the functional assembly of the Na(+)-driven flagellar motor in Vibrio alginolyticus. vibal Vibrio alginolyticus pull down pull down western blot western blot Immuno blot Immuno blot Takekawa N., Li N., Kojima S., Homma M. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation poma_vibal Chemotaxis protein PomA pomA protein protein vibal Vibrio alginolyticus MDLATLLGLIGGFAFVIMAMVLGGSIGMFVDVTSILIVVGGSIFVVLMKFTMGQFFGATKIAGKAFMFKADEPEDLIAKIVEMADAARKGGFLALEEMEINNTFMQKGIDLLVDGHDADVVRAALKKDIALTDERHTQGTGVFRAFGDVAPAMGMIGTLVGLVAMLSNMDDPKAIGPAMAVALLTTLYGAILSNMVFFPIADKLSLRRDQETLNRRLIMDGVLAIQDGQNPRVIDSYLKNYLNEGKRALEIDE o06874_vibal pomB Vag1382_06400 protein protein vibal Vibrio alginolyticus MDDEDNKCDCPPPGLPLWMGTFADLMSLLMCFFVLLLSFSEMDVLKFKQIAGSMKFAFGVQNQLEVKDIPKGTSIIAQEFRPGRPEPTPIDVIMQQTMDITQQTLEFHEGESDRAGGTKRDEGKLTGGQSPATSTQNNESAEADMQQQQSKEMSQEMETLMESIKKALEREIEQGAIEVENLGQQIVIRMREKGAFPEGSAFLQPKFRPLVRQIAELVKDVPGIVRVSGHTDNRPLDSELYRSNWDLSSQRAVSVAQEMEKVRGFSHQRLRVRGMADTEPLLPNDSDDNRALNRRVEISIMQGEPLYSEEVPVIQ pomb-poma 1339623 not-defined-192069750 1339624 unspecified role unspecified role prey prey O06873:p.Lys203Glu O06873-176-176 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false O06873:p.Gly157Asp O06873-157-157 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false not-defined-1865442173 1339625 unspecified role unspecified role bait bait o06874-- O06874-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 4