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    <entry>
        <source releaseDate="2012-12-17Z">
            <names>
                <shortLabel>mpidb</shortLabel>
                <fullName>The Microbial Protein Interaction Database</fullName>
            </names>
            <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0903" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6390654" refType="identity" refTypeAc="MI:0356"/>
            </xref>
        </source>
        <experimentList>
            <experimentDescription id="1339498">
                <names>
                    <shortLabel>hung-2012-1</shortLabel>
                    <fullName>Oligomerization of the response regulator ComE from Streptococcus mutans is affected by phosphorylation.</fullName>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="22210762" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </bibref>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6417940" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-18439" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="562">
                        <names>
                            <shortLabel>ecolx</shortLabel>
                            <fullName>Escherichia coli</fullName>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>crosslink</shortLabel>
                        <fullName>cross-linking study</fullName>
                        <alias typeAc="MI:1041" type="synonym">crosslink</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0030" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1049" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>weight by staining</shortLabel>
                        <fullName>molecular weight estimation by staining</fullName>
                        <alias typeAc="MI:1041" type="synonym">weight by staining</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0816" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218615" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Hung D.C., Downey J.S., Kreth J., Qi F., Shi W., Cvitkovitch D.G., Goodman S.D.</attribute>
                    <attribute name="exp-modification" nameAc="MI:0627">Cross-linking reagent, DMS. proteisn stained with Ni-NTA-Atto conjugate staining, specific for His-tagged proteins.</attribute>
                    <attribute name="journal" nameAc="MI:0885">J. Bacteriol. (0021-9193)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2012</attribute>
                    <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
                    <attribute name="imex curation" nameAc="MI:0959"/>
                    <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="1339499">
                <names>
                    <shortLabel>q8ds93_strmu</shortLabel>
                    <alias typeAc="MI:0301" type="gene name">comE</alias>
                    <alias typeAc="MI:0305" type="locus name">SMU_1917</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q8DS93" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0003677" secondary="DNA binding" version="TrEMBL_115"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001789"/>
                    <secondaryRef db="ensemblbacteria" id="AAN59528" version="TrEMBL_115" refType="transcript"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="WP_002262114.1"/>
                    <secondaryRef db="ensemblbacteria" id="AAN59528" version="TrEMBL_115" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR007492"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0045892" secondary="negative regulation of transcription, DNA-templated" version="TrEMBL_115"/>
                    <secondaryRef db="ensemblbacteria" id="SMU_1917" version="TrEMBL_115" refType="gene"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0000160" secondary="phosphorelay signal transduction system" version="TrEMBL_115"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_722222.1"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR039420"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6417945" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011006"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="210007">
                    <names>
                        <shortLabel>strain atcc 700610 /</shortLabel>
                        <fullName>Streptococcus mutans serotype c</fullName>
                    </names>
                </organism>
                <sequence>MISIFVLEDDFLQQGRLETTIAAIMKEKNWSYKELTIFGKPQQLIDAIPEKGNHQIFFLDIEIKKEEKKGLEVANQIRQHNPSAVIVFVTTHSEFMPLTFQYQVSALDFIDKSLNPEEFSHRIESALYYAMENSQKNGQSEELFIFHSSETQFQVPFAEILYFETSSTAHKLCLYTYDERIEFYGSMTDIVKMDKRLFQCHRSFIVNPANITRIDRKKRLAYFRNNKSCLISRTKLTKLRAVIADQRRAK</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction imexId="IM-18439-1" id="1339500">
                <names>
                    <shortLabel>come-come</shortLabel>
                </names>
                <xref>
                    <primaryRef db="mpidb" dbAc="MI:0903" id="MPIDB-INT-1781" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-18439-1" refType="imex-primary" refTypeAc="MI:0662"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6417950" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <experimentList>
                    <experimentRef>1339498</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="1339501">
                        <names>
                            <shortLabel>not-defined-708055714</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-6417954" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>1339499</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>unspecified role</shortLabel>
                                    <fullName>unspecified role</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <featureList>
                            <feature id="1339502">
                                <names>
                                    <shortLabel>Q8DS93:p.Asp60Glu</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6428971" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>mutation disrupting strength</shortLabel>
                                        <fullName>mutation disrupting interaction strength</fullName>
                                        <alias typeAc="MI:1041" type="synonym">mutation disrupting strength</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1128" refType="identity" refTypeAc="MI:0356"/>
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                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528001" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </startStatus>
                                        <begin position="60"/>
                                        <endStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
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                                            </xref>
                                        </endStatus>
                                        <end position="60"/>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                            <feature id="1339503">
                                <names>
                                    <shortLabel>Q8DS93:p.Asp60Ala</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6428969" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>mutation disrupting strength</shortLabel>
                                        <fullName>mutation disrupting interaction strength</fullName>
                                        <alias typeAc="MI:1041" type="synonym">mutation disrupting strength</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1128" refType="identity" refTypeAc="MI:0356"/>
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                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528001" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
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                                            </xref>
                                        </startStatus>
                                        <begin position="60"/>
                                        <endStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
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                                            </xref>
                                        </endStatus>
                                        <end position="60"/>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                            <feature id="1339504">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-6428967" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>his tag</shortLabel>
                                        <fullName>his tag</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">6-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">6-His-tag</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
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                                        </startStatus>
                                        <endStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
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                                            </xref>
                                        </endStatus>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="figure legend" nameAc="MI:0599">figure 6</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
