mpidb The Microbial Protein Interaction Database paul-2011a-1 Adjusting the spokes of the flagellar motor with the DNA-binding protein H-NS. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Paul K., Carlquist W.C., Blair D.F. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation flig_ecoli Flagellar motor switch protein FliG fliG fla AII.2 fla BII b1939 JW1923 protein protein ecoli Escherichia coli (strain K12) MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANVTQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRDTASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLRIATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAVREFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLRNMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV stpa_ecoli DNA-binding protein StpA stpA hnsB b2669 H-NS homolog StpA JW2644 protein protein ecoli Escherichia coli (strain K12) MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEEEQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDVNGETKTWTGQGRTPKPIAQALAEGKSLDDFLI hns_ecoli DNA-binding protein H-NS hns bglY cur drdX hnsA msyA osmZ pilG topS b1237 Histone-like protein HLP-II Protein H1 Protein B1 JW1225 Heat-stable nucleoid-structuring protein protein protein ecoli Escherichia coli (strain K12) MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ stpa-flig 1339032 not-defined-1735565179 1339033 unspecified role unspecified role prey prey P0ABZ1:p.Arg160Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0ABZ1:p.Glu125Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0ABZ1:p.His126Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli not-defined-1115898776 1339034 unspecified role unspecified role bait bait region P0ACG1-- gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli physical association physical association false false false figure 3C-E hns-flig 1339032 not-defined-807839352 1339035 unspecified role unspecified role bait bait P0ACF8:p.Gln112Asp P0ACF8-112-112 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P0ACF8:p.Arg114Trp P0ACF8-1-71 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region P0ACF8-64-137 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false P0ACF8:p.Gly113Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli not-defined-679002968 1339033 unspecified role unspecified role prey prey P0ABZ1:p.Arg160Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0ABZ1:p.His126Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0ABZ1:p.Glu125Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0ABZ1:p.Gln128Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false figure 2A, 2C, 3C