mpidb The Microbial Protein Interaction Database hsieh-2011-1 Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Hsieh F.C., Chen C.T., Weng Y.T., Peng S.S., Chen Y.C., Huang L.Y., Hu H.T., Wu Y.L., Lin N.C., Wu W.F. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation hsieh-2011-2 Stepwise activity of ClpY (HslU) mutants in the processive degradation of Escherichia coli ClpYQ (HslUV) protease substrates. ecolx Escherichia coli pull down pull down western blot western blot Immuno blot Immuno blot Hsieh F.C., Chen C.T., Weng Y.T., Peng S.S., Chen Y.C., Huang L.Y., Hu H.T., Wu Y.L., Lin N.C., Wu W.F. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation sula_ecoli Cell division inhibitor SulA sulA sfiA b0958 JW0941 protein protein ecoli Escherichia coli (strain K12) MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPMMTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVESMVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSGLKIHSNLYH hslu_ecoli ATP-dependent protease ATPase subunit HslU hslU htpI b3931 JW3902 Unfoldase HslU Heat shock protein HslU protein protein ecoli Escherichia coli (strain K12) MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEELRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFRKKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKIKDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQALTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGARRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL hslu-sula-1 1338784 not-defined-1247108388 1338786 unspecified role unspecified role prey prey P0AFZ5:p.Met89Ile P0AFZ5-89-89 mutation mutation certain certain sequence position certain certain certain sequence position certain false not-defined-1582685463 1338787 unspecified role unspecified role bait bait P0A6H5:p.Gly90Asp P0A6H5-90-90 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 2, 3, 5 and 6 hslu-sula-2 1338785 not-defined-1288677317 1338786 unspecified role unspecified role prey prey not-defined-325047647 1338787 unspecified role unspecified role bait bait P0A6H5:p.Gly90Asp P0A6H5-90-90 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false P0A6H5:p.Tyr91Phe P0A6H5-91-91 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false P0A6H5:p.Glu266Gln P0A6H5-266-266 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false P0A6H5:p.Tyr408Ala P0A6H5-408-408 mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false p0a6h5-- P0A6H5-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false figure 7