mpidb The Microbial Protein Interaction Database thakor-2011-1 Identification of an anchor residue for CheA-CheY interactions in the chemotaxis system of Escherichia coli. in vitro In vitro fluorescence fluorescence technology fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence Thakor H., Nicholas S., Porter I.M., Hand N., Stewart R.C. The rapid decrease in fluorescence following mixing of P-CheA with CheY reflects decreased emission signal from CheY residue trp-58 as the protein becomes phosphorylated on Asp-57. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation thakor-2011-2 Identification of an anchor residue for CheA-CheY interactions in the chemotaxis system of Escherichia coli. in vitro In vitro phosphotransferase phosphotransferase assay phosphotransferase predetermined predetermined participant predetermined Thakor H., Nicholas S., Porter I.M., Hand N., Stewart R.C. The rapid decrease in fluorescence following mixing of P-CheA with CheY reflects decreased emission signal from CheY residue trp-58 as the protein becomes phosphorylated on Asp-57. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation chey_ecoli Chemotaxis protein CheY cheY b1882 JW1871 protein protein ecoli Escherichia coli (strain K12) MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM chea_ecoli Chemotaxis protein CheA cheA b1888 JW1877 protein protein ecoli Escherichia coli (strain K12) MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIFRAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQLDAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPRRIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEADQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLINLVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFSRYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKRLAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEVAMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQKMGGHVEIQSKQGTGTTIRILLPLTLAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWKVFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAATILGDGSVALIVDVSALQAINREQRMANTAA chey-chea-4 1338602 not-defined-1080777322 1338604 unspecified role unspecified role bait bait p0ae67-- P0AE67-- fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-995640537 1338605 unspecified role unspecified role prey prey P07363:p.Glu178Ala P07363-178-178 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region P07363-159-227 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P07363:p.Phe214Ala P07363-214-214 mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P07363:p.Ile216Ala P07363-216-216 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P07363:p.Glu171Ala P07363-171-171 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli 1338602 direct interaction direct interaction false false false figure 2 chea-chey-3 1338603 not-defined-1487412840 1338604 phosphate acceptor phosphate acceptor unspecified role unspecified role not-defined-1069021099 1338605 phosphate donor phosphate donor unspecified role unspecified role region P07363-- phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P07363:p.Phe214Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false phosphotransfer phosphotransfer reaction phosphotransfer false false false figure 4