mpidb The Microbial Protein Interaction Database luo-2011c-1 Interactions and predicted host membrane topology of the enteropathogenic Escherichia coli translocator protein EspB. eco27 Escherichia coli O127:H6 (strain E2348/69 / EPEC) pull down pull down western blot western blot Immuno blot Immuno blot Luo W., Donnenberg M.S. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation eaeb_eco27 Protein EaeB eaeB espB E2348C_3934 protein protein eco27 Escherichia coli O127:H6 (strain E2348/69 / EPEC) MNTIDNNNAAIAVNSVLSSTTDSTSSTTTSTSSISSSLLTDGRVDISKLLLEVQKLLREMVTTLQDYLQKQLAQSYDIQKAVFESQNKAIDEKKAGATAALIGGAISSVLGILGSFAAINSATKGASDVAQQAASTSAKSIGTVSEASTKALAKASEGIADAADDAAGAMQQTIATAAKAASRTSGITDDVATSAQKASQVAEEAADAAQELAQKAGLLSRFTAAAGRISGSTPFIVVTSLAEGTKTLPTTISESVKSNHDINEQRAKSVENLQASNLDTYKQDVRRAQDDISSRLRDMTTTARDLTDLINRMGQAARLAG b7um93_eco27 espD E2348C_3935 protein protein eco27 Escherichia coli O127:H6 (strain E2348/69 / EPEC) MLNVNNDIQSVRSGASAATATSGINQSEVTSALDLQLVKSTAPSASWTESTALPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEGTEPQRSTQNASSGYPYLSQVNNVDPQAMMMMATLLSLDASAQRVASMKNSNEIYADGQNKALDNKTLEFKKQLEEQQKAEEKAQKSKIVGQVFGWLGVAATAIAAIFNPALWAVVAISATAMALQTAVDVMGDDAPQALKTAAQAFGGLSLAAGILTAGIGGVSSLISKVGDVANKVGSNIVKVVTTLADTFVDNVASKISAVANGLTTSSRSIGTTVLNNDAAYYNVLSQVSAFAVENLTRQSEYLSQSAKAELEKATLELQNQANYIQSASQLMSDSARVNIRIVSGRV b7um94_eco27 espA E2348C_3936 protein protein eco27 Escherichia coli O127:H6 (strain E2348/69 / EPEC) MDTSTTASVASANASTSTSMAYDLGSMSKDDVIDLFNKLGVFQAAILMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNAKAQLPDEVISYINDPRNDITISGIDNINAQLGAGDLQTVKAAISAKANNLTTTVNNSQLEIQQMSNTLNLLTSARSDMQSLQYRTISGISLGK eaeb-espd 1338425 not-defined-298714200 1338426 unspecified role unspecified role bait bait Q05129:p.Ile78_Gln79insXaa Q05129-78-78 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q05129:p.Ser75_Tyr76insXaa Q05129-75-75 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false q05129-186-186 Q05129-186-186 his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag certain certain sequence position certain certain certain sequence position certain false Q05129:p.Ala166_Ala167insXaa Q05129-166-166 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false not-defined-966922355 1338427 unspecified role unspecified role prey prey physical association physical association false false false Figure 2 espd-eaeb-1 1338425 n/a 1338428 unspecified role unspecified role prey prey not-defined-1278194494 1338426 unspecified role unspecified role prey prey not-defined-1181503216 1338427 unspecified role unspecified role bait bait b7um93-32-46 B7UM93-32-46 his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag certain certain sequence position certain certain certain sequence position certain false association association false false false Figure 2A espa-espd 1338425 not-defined-982751381 1338428 unspecified role unspecified role bait bait region B7UM94-123-123 his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag certain certain sequence position certain certain certain sequence position certain false not-defined-1753998903 1338427 unspecified role unspecified role prey prey physical association physical association false false false Figure 2B