mpidb The Microbial Protein Interaction Database masi-2010-1 Multiple signals direct the assembly and function of a type 1 secretion system. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay western blot western blot Immuno blot Immuno blot Masi M., Wandersman C. cwander@pasteur.fr J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation hasa_serma Hemophore HasA Heme acquisition system protein A hasA protein protein serma Serratia marcescens MAFSVNYDSSFGGYSIHDYLGQWASTFGDVNHTNGNVTDANSGGFYGGSLSGSQYAISSTANQVTAFVAGGNLTYTLFNEPAHTLYGQLDSLSFGDGLSGGDTSPYSIQVPDVSFGGLNLSSLQAQGHDGVVHQVVYGLMSGDTGALETALNGILDDYGLSVNSTFDQVAAATAVGVQHADSPELLAA q53368_serma hasD prtD protein protein serma Serratia marcescens MSVLAAYRRGFWGIALFTAVINLLMLAPALYMLQVYDRVLPSGNRMTLAMLTLMVVGLYLFMGLLEWVRSQVVIRLGAQMDMRLNQRVYDAAFETNLKTGNPLAGQALNDLTNLRQFATGNALFAFFDAPWFPVYLLVVFLLHPWLGALASAGCDRAGAAGLAQSAGCRKRLSAEARPGSRCAHTAGQPANLRNAEAIAAMGMLTDLRLRWLRQHQQFLLLQNRASEKIAAVTAWSKTVRLALQSLMLGCGALLAVSGDITPGMMIAGSILIGRVLGPIDQLIGAWKQWSSARQSLQRLEVMLAANPPRIPSLPLPAPGGALTVSQLTASAPGGTAPVLHGVSFRLEAGEVLGVIGASGSGKTLLMRQLVGALTPISGDGGAEQLGPHIGYLPQDIQLFAGTLTDNIARFGQVDAEKVVAAAALAGVHQLILHLPKGYETELGEGGSGLSGGQRQRVALARALYGSPALVVLDEPNANLDREGEEALQRAIEALKARGNTIVLVTHKPAILATTDKLLVLTAGQVQHFGPSDAILKKLPGFAPAAAVAPANTGRSNGGFNVNYANFAKTASGERKV prtd-hasa-1 1337410 not-defined-1622236172 1337411 unspecified role unspecified role prey prey Q54450:p.[Tyr46_Gly47insXaaXaaXaaXaaXaa;Ser107_Ile108insXaaXaaXaaXaaXaa] Q54450-46-46 mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength insertion analysis insertion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region Q54450-46-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q54450:p.[Glu148Ala;Asp167Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli not-defined-453978273 1337412 unspecified role unspecified role bait bait n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false Figure 4